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Reply to: "CD8+ T cell diversification by asymmetric cell division"

Nature Immunology volume 16, pages 893–894 (2015)Cite this article

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Arsenio et al. respond:

The goal of our recent work1 was to trace the transcriptional 'roadmap' of individual CD8+ T lymphocytes as they differentiated in vivo from the naive state into one of three 'fates': terminally differentiated, short-lived effector (TSLE) cells (KLRG1hiIL-7Rlo); central memory (TCM) cells (CD44hiCD62Lhi); and effector memory (TEM) cells (CD44hiCD62Llo). We performed single-cell gene-expression analyses on individual CD8+ T lymphocytes isolated at multiple time points (naive, cell division 1, day 3, day 5, day 7 TSLE, day 45 TEM, day 45 TCM) after microbial infection in vivo. This work suggested that an early divergence in lymphocyte fates may occur during an immune response to microbial infection.

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References

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Acknowledgements

Supported by the US National Institutes of Health (OD008469 and AI095277 to J.T.C. and HG004659 and NS076449 to G.W.Y.).

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Authors and Affiliations

  1. Department of Medicine, University of California San Diego, La Jolla, CA, USA

    Janilyn Arsenio, Patrick J Metz & John T Chang

  2. Department of Cellular and Molecular Medicine, UCSD Stem Cell and Bioinformatics Programs, and Institute for Genomic Medicine, University of California San Diego, La Jolla, CA, USA

    Boyko Kakaradov & Gene W Yeo

  3. Department of Physiology, National University of Singapore and Genome Institute of Singapore and Molecular Engineering Laboratory, A*STAR, Singapore

    Gene W Yeo

Authors
  1. Janilyn Arsenio
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  2. Boyko Kakaradov
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  3. Patrick J Metz
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  4. Gene W Yeo
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  5. John T Chang
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Corresponding authors

Correspondence to Gene W Yeo or John T Chang.

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Arsenio, J., Kakaradov, B., Metz, P. et al. Reply to: "CD8+ T cell diversification by asymmetric cell division". Nat Immunol 16, 893–894 (2015). https://doi.org/10.1038/ni.3234

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