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Quantitative shotgun proteomics: considerations for a high-quality workflow in immunology

Proteomics based on high-resolution mass spectrometry has become a powerful tool for the analysis of protein abundance, modifications and interactions. Here we describe technical aspects of proteomics workflows, instrumentation as well as computational considerations to obtain high-quality proteomics data.

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Figure 1: Cellular and immunological processes that can be studied using proteomics tools.
Figure 2: Shotgun proteomics workflow.
Figure 3: Strategies for quantification of peptides from MS1 spectra.
Figure 4: Computational data analysis.

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Correspondence to Felix Meissner or Matthias Mann.

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Meissner, F., Mann, M. Quantitative shotgun proteomics: considerations for a high-quality workflow in immunology. Nat Immunol 15, 112–117 (2014). https://doi.org/10.1038/ni.2781

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