Abstract
Toll-like receptor (TLR) signaling activates the inhibitor of transcription factor NF-κB (IκB) kinase (IKK) complex, which governs NF-κB-mediated transcription during inflammation. The RNase regnase-1 serves a critical role in preventing autoimmunity by controlling the stability of mRNAs that encode cytokines. Here we show that the IKK complex controlled the stability of mRNA for interleukin 6 (IL-6) by phosphorylating regnase-1 in response to stimulation via the IL-1 receptor (IL-1R) or TLR. Phosphorylated regnase-1 underwent ubiquitination and degradation. Regnase-1 was reexpressed in IL-1R- or TLR-activated cells after a period of lower expression. Regnase-1 mRNA was negatively regulated by regnase-1 itself via a stem-loop region present in the regnase-1 3′ untranslated region. Our data demonstrate that the IKK complex phosphorylates not only IκBα, thereby activating transcription, but also regnase-1, thereby releasing a 'brake' on IL-6 mRNA expression.
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Acknowledgements
We thank laboratory colleagues; E. Kamada and M. Kageyama for secretarial assistance; Y. Fujiwara, M. Kumagai and N. Umano for technical assistance; M. Takahama and T. Misawa for help with experiments; A. Matsuo, T. Hata, M. Tanaka and T. Kurimoto for discussions; K. Iwai (Osaka University) for expression plasmids for Flag-tagged β-TrCP and β-TrCPΔF; and D.V. Goeddel (Tularik; present affiliation, NGM Biopharmaceuticals) for the expression plasmid for IKKβ; I. Verma and M. Schmitt (Salk Institute), M. Schmidt-Supprian (Max Planck Institute) for MEFs deficient in IKKβ or both IKKα and IKKβ; S. Yamaoka (Tokyo Medical and Dental University) for Rat-1 and 5R cells; and F. Inagaki (Hokkaido University) for recombinant regnase-1 (amino acids 1–330). Supported by the Special Coordination Funds of the Japanese Ministry of Education, Culture, Sports, Science and Technology, and the Ministry of Health, Labour and Welfare in Japan, the Japan Society for the Promotion of Science through Funding Program for World-Leading Innovative R&D on Science and Technology (FIRST Program).
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H.I. and O.T. designed and did most of the experiments and analyzed the data; K.M., T.U., K.K., T. Satoh and T. Saitoh helped with experiments; S.T. and D.M.S. did mathematical modeling; M.M. provided advice for experiments; H.I., S.T. and O.T. wrote the manuscript; and S.A. supervised the project.
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Iwasaki, H., Takeuchi, O., Teraguchi, S. et al. The IκB kinase complex regulates the stability of cytokine-encoding mRNA induced by TLR–IL-1R by controlling degradation of regnase-1. Nat Immunol 12, 1167–1175 (2011). https://doi.org/10.1038/ni.2137
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DOI: https://doi.org/10.1038/ni.2137
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