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Reply to "Failure to detect production of IL-10 by activated human neutrophils"

Nature Immunology volume 12, pages 1018–1020 (2011)Cite this article

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Cerundolo et al. respond:

In our article, we reported that human neutrophils can secrete IL-10 and that their interaction with iNKT cells can result in less IL-10 secretion1, thereby providing therapeutic opportunities that should be explored in future clinical trials. Davey et al. have described a series of experiments in which they did not detect the secretion of IL-10 by purified neutrophils, and on the basis of such negative results they conclude that human neutrophils are unable to produce IL-10. This is in contrast with results we have been generating routinely for more than 3 years and with other published data demonstrating the ability of human neutrophils2,3, mouse neutrophils4,5 and equine neutrophils6 to secrete IL-10, which indicates a common cross-species strategy for down-modulation of the proinflammatory activity of neutrophils. Indeed, a published paper has demonstrated that infiltrating neutrophils in mice infected with Yersinia enterocolitica represent most IL-10-producing cells at the site of infection7. As our results were highly reproducible and were not due to the presence of contaminating monocytes (Supplementary Note and Supplementary Figs. 1–3), we can only conclude that the negative results obtained by Davey et al. were caused by their purification protocols and the experimental conditions they used that diminished the ability of their preparations of human neutrophils to secrete IL-10.

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Figure 1: Importance of timing in the purification and activation of human neutrophils for the detection of IL-10.

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Authors and Affiliations

  1. Nuffield Department of Medicine, Medical Research Council Human Immunology Unit, John Radcliffe Hospital, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK

    Carmela De Santo, Mariolina Salio, Tao Dong & Vincenzo Cerundolo

  2. Faculty of Biology, Technion-Israel Institute of Technology, Haifa, Israel

    Yoram Reiter

Authors
  1. Carmela De Santo
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  2. Mariolina Salio
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  3. Tao Dong
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  4. Yoram Reiter
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  5. Vincenzo Cerundolo
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Corresponding author

Correspondence to Vincenzo Cerundolo.

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The authors declare no competing financial interests.

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Supplementary Figures 1–9, Note and Methods (PDF 5049 kb)

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De Santo, C., Salio, M., Dong, T. et al. Reply to "Failure to detect production of IL-10 by activated human neutrophils". Nat Immunol 12, 1018–1020 (2011). https://doi.org/10.1038/ni.2132

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