Abstract
Phosphonates, compounds with a carbon–phosphorus bond, are a key component of the marine dissolved organic phosphorus pool1. These compounds serve as a phosphorus source for primary producers, including the nitrogen-fixing cyanobacteria Trichodesmium2. Phosphonates can therefore support marine primary production, as well as climate-driven increases in marine nitrogen fixation3, carbon sequestration4 and possibly methane production, through the breakdown of methylphosphonate5. Despite their importance, the source of phosphonates to the open ocean has remained uncertain. Here, we use solid-state nuclear magnetic resonance spectroscopy to screen for the presence of phosphonates in cultured strains of Trichodesmium erythraeum. We show that phosphonates comprise an average of 10% of the cellular particulate phosphorus pool in this species. We therefore suggest that these cyanobacteria produce phosphonates, and might be a significant source of these compounds in the ocean, particularly in nutrient-poor regions, where Trichodesmium blooms occur. Given that Trichodesmium also thrives in a warm, carbon-dioxide-rich environment3, phosphonate production may increase in the future. This, in turn, might select for a microbial community that can use phosphonate, and could have implications for nitrogen fixation, carbon sequestration and greenhouse-gas production.
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Acknowledgements
This work was supported by the National Science Foundation Biological and Chemical Oceanography Programs, the Center for Microbial Oceanography: Research and Education, and the Woods Hole Oceanographic Institution. The authors thank B. A. S. Van Mooy and E. Ingall for their helpful discussions, J. Waterbury for access to cultures and the participants of the ATP-3 project for assistance at sea.
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S.T.D. and C.R.B.-N. conceived of the study, processed samples and wrote the manuscript. E.D.O. and S.T.H. carried out the culture studies and P.J.P. did the 31P NMR spectroscopy.
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Dyhrman, S., Benitez-Nelson, C., Orchard, E. et al. A microbial source of phosphonates in oligotrophic marine systems. Nature Geosci 2, 696–699 (2009). https://doi.org/10.1038/ngeo639
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DOI: https://doi.org/10.1038/ngeo639
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