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Genetic analysis of the mouse brain proteome

Nature Genetics volume 30, pages 385–393 (2002)Cite this article

Abstract

Proteome analysis is a fundamental step in systematic functional genomics. Here we have resolved 8,767 proteins from the mouse brain proteome by large-gel two-dimensional electrophoresis. We detected 1,324 polymorphic proteins from the European collaborative interspecific backcross. Of these, we mapped 665 proteins genetically and identified 466 proteins by mass spectrometry. Qualitatively polymorphic proteins, to 96%, reflect changes in conformation and/or mass. Quantitatively polymorphic proteins show a high frequency (73%) of allele-specific transmission in codominant heterozygotes. Variations in protein isoforms and protein quantity often mapped to chromosomal positions different from that of the structural gene, indicating that single proteins may act as polygenic traits. Genetic analysis of proteomes may detect the types of polymorphism that are most relevant in disease-association studies.

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Figure 1: Protein phenotypes determined by 2-DE.
Figure 2: Genetic map of mouse chromosome 3 (MMU3).
Figure 3: 2-DE brain protein pattern from a B6-SPR hybrid.

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Author information

Authors and Affiliations

  1. Institut für Humangenetik, Humboldt-Universität zu Berlin, Charité, Campus Virchow-Klinikum, Augustenburger Platz 1, Berlin, D-13353, Germany

    Joachim Klose, Christina Nock & Marion Herrmann

  2. Fachbereich Biologie, Chemie, Pharmazie, Freie Universität Berlin, Berlin, D-14195, Germany

    Christina Nock

  3. Max-Planck-Institut für Molekulare Genetik, Ihnestrasse 73, Berlin, D-14195, Germany

    Christina Nock, Konrad Büssow, Heinz Himmelbauer & Hans Lehrach

  4. Institut für Physiologische Chemie, Ruhr-Universität Bochum, Bochum, D-44780, Germany

    Kai Stühler & Katrin Marcus

  5. Protagen AG, Bochum, D-44801, Germany

    Martin Blüggel

  6. Institut für Molekulare Pharmakologie, Berlin, D-13125, Germany

    Eberhard Krause

  7. SGDP Research Centre, Institute of Psychiatry, London, SE5 8AF, UK

    Leonard C. Schalkwyk

  8. MWB Business Exchange Suite 112/113 Wellington House, Cambridge, CB1 1BH, UK

    Sohaila Rastan

  9. MRC Mammalian Genetics Unit and UK Mouse Genome Centre, Harwell, OX11 ORD, Oxford, UK

    Steve D.M. Brown

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  1. Joachim Klose
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Correspondence to Joachim Klose or Heinz Himmelbauer.

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Klose, J., Nock, C., Herrmann, M. et al. Genetic analysis of the mouse brain proteome. Nat Genet 30, 385–393 (2002). https://doi.org/10.1038/ng861

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