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Targeted breakage of a human chromosome mediated by cloned human telomeric DNA

Nature Genetics volume 2, pages 283–287 (1992)Cite this article

Abstract

Novel approaches to the structural and functional analysis of mammalian chromosomes would be possible if the gross structure of the chromosomes in living cells could be engineered. Controlled modifications can be engineered by conventional targeting techniques based on homologous recombination. Large but uncontrolled modifications can be made by the integration of cloned human telomeric DNA. We describe here the combined use of gene targeting and telomere–mediated chromosome breakage to generate a defined truncation of a human chromosome. Telomeric DNA was targeted to the 6–16 gene on the short arm of chromosome 1 in a human cell line. Molecular and cytogenetic analyses showed that, of eight targeted clones that were isolated, one clone had the predicted truncation of chromosome 1.

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Authors and Affiliations

  1. Department of Biochemistry, Oxford University, South Parks Road, Oxford, OX1 3QU, UK

    Jane E. Itzhaki, Michael A. Barnett, William R.A. Brown & Andrew C.G. Porter

  2. Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, OX3 9DU, UK

    Angela B. MacCarthy & Veronica J. Buckle

Authors
  1. Jane E. Itzhaki
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  2. Michael A. Barnett
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  3. Angela B. MacCarthy
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  4. Veronica J. Buckle
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  5. William R.A. Brown
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  6. Andrew C.G. Porter
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Itzhaki, J., Barnett, M., MacCarthy, A. et al. Targeted breakage of a human chromosome mediated by cloned human telomeric DNA. Nat Genet 2, 283–287 (1992). https://doi.org/10.1038/ng1292-283

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