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Complete coverage of the Schizosaccharomyces pombe genome in yeast artificial chromosomes

Nature Genetics volume 1, pages 273–277 (1992)Cite this article

Abstract

The genome of the fission yeast, Schizosaccharomyces pombe, consists of some 14 million base pairs of DNA contained in three chromosomes. On account of its excellent genetics we used it as a test system for a strategy designed to map mammalian chromosomes and genomes. Data obtained from hybridization fingerprinting established an ordered library of 1,248 yeast artificial chromosome clones with an average size of 535 kilobases. The clones fall into three contigs completely representing the three chromosomes of the organism. This work provides a high resolution physical and clone map of the genome, which has been related to available genetic and physical map information.

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References

  1. Kaufer, N.F., Simanis, V. & Nurse, P. Nature 318, 78–80 (1985).

    Article  CAS  PubMed  Google Scholar 

  2. Russell, P. & Nurse, P. Cell 45, 781–782 (1986).

    Article  CAS  PubMed  Google Scholar 

  3. Allshire, R.C. et al. Cell 50, 391–403 (1987).

    Article  CAS  PubMed  Google Scholar 

  4. Lee, M.G. & Nurse, P. Nature 327, 31–35 (1987).

    Article  CAS  PubMed  Google Scholar 

  5. Uemura, T. et al. Cell 50, 917–925, (1987).

    Article  CAS  PubMed  Google Scholar 

  6. Booher, B. & Beach, D. Cell 57, 1009–1016 (1989).

    Article  CAS  PubMed  Google Scholar 

  7. Moreno, S., Hayles, J. & Nurse, P. Cell 58, 361–372 (1989).

    Article  CAS  PubMed  Google Scholar 

  8. Kinoshita, N., Ohkura, H. & Yanagida, M. Cell 63, 405–415 (1990).

    Article  CAS  PubMed  Google Scholar 

  9. Kohli, J. Current Genetics 11, 575–589 (1987).

    Article  CAS  PubMed  Google Scholar 

  10. Munz, P., Wolf, K., Kohli, J. & Leupold, U. Molecular Biology of the Fission Yeast (eds Nasim, A. et al.) 1–30 (Academic Press, San Diego, 1989).

    Book  Google Scholar 

  11. Fan, J. et al. Nuc. Acid. Res. 17, 2801–2818 (1989).

    Article  CAS  Google Scholar 

  12. Burke, D.T., Carle, G.F. & Olson, M.V. Science 236, 806–812 (1987).

    Article  CAS  PubMed  Google Scholar 

  13. Lehrach, H. et al. In Genome Analysis: Genetic and Physical Mapping (eds Davies, K. E. & Tilghman, S.) 39–81 (Cold Spring Harbor Laboratory Press, New York, 1990).

    Google Scholar 

  14. Olson, M.V., Hood, L., Cantor, C.R. & Botstein, D. Science 245, 1434–1435 (1989).

    Article  CAS  PubMed  Google Scholar 

  15. Chikashige, Y. et al. Cell 57, 739–751 (1989).

    Article  CAS  PubMed  Google Scholar 

  16. Schaak, J., Mao, J. & Söll, D. Nuc.Acid. Res. 10, 2851–2864 (1982).

    Article  CAS  Google Scholar 

  17. Levin, H.L., Weaver, D.C. & Boeke, J.D. Molec. cell. Biol. 10, 6791–6798 (1990).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Toda, T., Nakaseko, Y., Niwa, O. & Yanagida, M. Curr. Genet. 8, 93–97 (1984).

    Article  CAS  PubMed  Google Scholar 

  19. Fan, J., Grothues, D. & Smith, C.L. Nuc. Acid. Res. 19, 6289–6294 (1991).

    Article  CAS  Google Scholar 

  20. Kohli, J., Hottinger, H., Munz, P., Strauss, A. & Thuriaux, P. Genetics 87, 471–489 (1977).

    CAS  PubMed  PubMed Central  Google Scholar 

  21. Hirano, T., Funahashi, S., Uemura, T., Yanagida, M. EMBO J. 5, 2973–2979 (1986).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Broughton, B.C. et al. Molec. gen. Genet. 228, 470–472 (1991).

    Article  CAS  PubMed  Google Scholar 

  23. Riley, J. et al. Nuc. Acid. Res. 18, 2887–2890 (1990).

    Article  CAS  Google Scholar 

  24. Little, R.D., Pilia, G., Johnson, S., D'Urso, M.D. & Schlessinger, D. Proc. natn. Acad. Sci. U.S.A. 89, 177–181 (1992).

    Article  CAS  Google Scholar 

  25. Sternberg, N.L. Proc. natn. Acad. Sci. U.S.A. 87, 103–107 (1990).

    Article  CAS  Google Scholar 

  26. Oliver, S.G. et al. Nature 357, 38–46 (1992).

    Article  CAS  PubMed  Google Scholar 

  27. Larin, Z., Monaco, A.P. & Lehrach, H. Proc. natn. Acad. Sci. U.S.A. 88, 4123–4127 w(1991).

    Article  CAS  Google Scholar 

  28. Larin, Z. & Lehrach, H. Genet. Res. 56, 203–208 (1990).

    Article  CAS  PubMed  Google Scholar 

  29. Birnboim, H.C. & Doly, J. Nuc. Acid. Res. 7, 1513–1523 (1979).

    Article  CAS  Google Scholar 

  30. Feinberg, A.P. & Vogelstein, B. Analyt. Biochem. 132, 6–13 (1983).

    Article  CAS  PubMed  Google Scholar 

  31. Press, W.H., Flannery, B.P., Teukolsky, S.A. & Vetterling, W.T. Numerical Recipes in C 343–351 (Cambridge University Press, Cambridge, 1988).

    Google Scholar 

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Authors and Affiliations

  1. Genome Analysis Laboratory, Imperial Cancer Research Fund, P.O. Box 123, Lincolns Inn Fields, London, WC2A 3PX, UK

    Elmar Maier, Jörg D. Hoheisel, Linda McCarthy, Richard Mott, Andrei V. Grigoriev, Zora Larin & Hans Lehrach

  2. Human Genetics Laboratory, Imperial Cancer Research Fund, Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, OX3 9DU, UK

    Anthony P. Monaco

Authors
  1. Elmar Maier
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  2. Jörg D. Hoheisel
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  3. Linda McCarthy
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  4. Richard Mott
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  5. Andrei V. Grigoriev
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  6. Anthony P. Monaco
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  7. Zora Larin
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  8. Hans Lehrach
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Maier, E., Hoheisel, J., McCarthy, L. et al. Complete coverage of the Schizosaccharomyces pombe genome in yeast artificial chromosomes. Nat Genet 1, 273–277 (1992). https://doi.org/10.1038/ng0792-273

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