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Mitochondrial DNA heteroplasmy in cloned cattle produced by fetal and adult cell cloning

Nature Genetics volume 25, pages 255–257 (2000)Cite this article

Abstract

Mammals have been cloned from adult donor cells1,2,3. Here we report the first cases of mitochondrial DNA (mtDNA) heteroplasmy in adult mammalian clones generated from fetal and adult donor cells. The heteroplasmic clones included a healthy cattle equivalent of the sheep Dolly, for which a lack of heteroplasmy was reported4.

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Figure 1: Quantitation of heteroplasmy.

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Acknowledgements

We thank A. Pollack for use of the ABI PRISM 7700 Sequence Detection System; M.A. Nowshari, G. Mößlacher, H. Füllgrabe, A. Range, A. Bergthaler and P. Stojkovic for technical assistance; and S.J. Weiss for comments on the manuscript. This work was supported in part by Agrobiogen and the Deutsche Forschungsgemeinschaft (WO685/2-1 and WO685/3-1 to E.W.).

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Authors and Affiliations

  1. Institute of Animal Breeding Genetics, University of Veterinary Medicine, Vienna, Austria

    Ralf Steinborn, Pamela Schinogl, Mathias Müller & Gottfried Brem

  2. Ludwig-Boltzmann Institute for Immuno- Cyto- and Molecular Genetic Research, Vienna, Austria

    Ralf Steinborn, Roland Achmann & Gottfried Brem

  3. Department of Biotechnology in Animal Production, Institute for Agrobiotechnology, Tulln, Austria

    Gottfried Brem

  4. Department of Molecular Animal Breeding and Genetics, Gene Center, Ludwig-Maximilian University, Munich, Germany

    Valeri Zakhartchenko, Wolfgang Schernthaner, Miodrag Stojkovic & Eckhard Wolf

Authors
  1. Ralf Steinborn
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  2. Pamela Schinogl
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  3. Valeri Zakhartchenko
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  4. Roland Achmann
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  6. Miodrag Stojkovic
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  7. Eckhard Wolf
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  8. Mathias Müller
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  9. Gottfried Brem
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Correspondence to Ralf Steinborn.

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Steinborn, R., Schinogl, P., Zakhartchenko, V. et al. Mitochondrial DNA heteroplasmy in cloned cattle produced by fetal and adult cell cloning. Nat Genet 25, 255–257 (2000). https://doi.org/10.1038/77000

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