Abstract
A new approach for the isolation of chromosome–specific subsets from a human genomic yeast artificial chromosome (YAC) library is described. It is based on the hybridization with an Alu polymerase chain reaction (PCR) probe. We screened a 1.5 genome equivalent YAC library of megabase insert size with Alu PCR products amplified from hybrid cell lines containing human chromosome 21, and identified a subset of 63 clones representative of this chromosome. The majority of clones were assigned to chromosome 21 by the presence of specific STSs and in situ hybridization. Twenty–nine of 36 STSs that we tested were detected in the subset, and a contig spanning 20 centimorgans in the genetic map and containing 8 STSs in 4 YACs was identified. The proposed approach can greatly speed efforts to construct physical maps of the human genome.
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Chumakov, I., Le Gall, I., Billault, A. et al. Isolation of chromosome 21–specific yeast artificial chromosomes from a total human genome library. Nat Genet 1, 222–225 (1992). https://doi.org/10.1038/ng0692-222
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DOI: https://doi.org/10.1038/ng0692-222
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