Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

A genetic linkage map of the Syrian hamster and localization of cardiomyopathy locus on chromosome 9qa2.1–b1 using RLGS spot–mapping

Nature Genetics volume 13, pages 87–90 (1996)Cite this article

Abstract

The Syrian cardiomyopathic hamster1 (BIO14.6) has an inherited form of progressive myocardial necrosis and congestive heart failure. Although widely studied2–4 as an animal model for human hypertrophic cardiomyopathy, further genetic analysis has been limited by a scarcity of DMA markers. Until now, only six autosomal linkage groups have been described5 and the number of polymorphic loci was extremely limited. In this study, we applied the restriction landmark genome scanning (RLGS) spot-mapping method6–9 to construct a genetic map of the Syrian hamster (Mesocricetus auratus) using 72 back-cross progeny. Although the polymorphic rate is very low (3–7%) between the strains, 531 polymorphic spots/loci were mapped, showing the power of this approach and reasonable applicability to other organisms lacking a well-defined genetic map. Further, the spot markers which flank the cardiomyopathy (cm) locus were cloned to determine the chromosomal location of cm by fluorescent in situ hybridization (FISH) analysis, resulting in the assignment of the locus to the centromeric region of hamster chromosome 9qa2.1–b1. Several candidate genes responsible for hypertrophic cardiomyopathy in humans have been excluded.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Homburger, F. Baker, J.R., Nixon, C.W. & Whitney, R. Primary,generalized polymyopathy and cardiac necrosis in an inbred line of Syrian hamsters. Med. Exp. 6, 339–345 (1962).

    Google Scholar 

  2. Bajusz, E. & Homburger, F. Myopathies: a meeting report. Science 152, 1112–1113 (1966).

    Article  CAS  Google Scholar 

  3. Homburger, F. & Bajusz, E. New models of human disease in Syrian hamsters. J. Am. Med. Assoc. 212, 604–610 (1970).

    Article  CAS  Google Scholar 

  4. Wagner, J.A. et al. Calcium antagonist receptors in cardiomyopathic hamster: selective increases in heart, muscle, brain. Science 232, 515–518 (1986).

    Article  CAS  Google Scholar 

  5. Robinson, R. Mesocricetus auratus (Syrian Hamster). in Genetic maps: Locus maps of complex genomes 6th edn. (ed. O'Brien, S. J.) (Cold Spring Harbor Laboratory Press, New York, 1993).

    Google Scholar 

  6. Hatada, I. Hayashizaki, Y., Hirotsune, S., Komatsubara, K. & Mukai, T. A genomic scanning method for higher organisms using restriction sites as landmarks on the genome. Proc. Natl. Acad. Sci. USA 88, 9523–9527 (1991).

    Article  CAS  Google Scholar 

  7. Hayashizaki, Y. et al. A genetic linkage map of the mouse using restriction landmark genomic scanning (RLGS). Genetics 138, 1207–1238 (1994).

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Okuizumi, H. et al. Genetic mapping of restriction landmark genomic scanning (RLGS) loci in the mouse. Electrophoresis 16, 233–240 (1995).

    Article  CAS  Google Scholar 

  9. Okazaki, Y. et al. A genetic linkage map of the mouse using an expanded production system of restriction landmark genomic scanning (RLGS Ver.1.8). Biochem. Biophys. Res. Commun. 205, 1922–1929 (1994).

    Article  CAS  Google Scholar 

  10. Manly, K.F. A Macintosh program for storage and analysis of experimental genetic mapping data. Mamm. Genome 4, 303–313 (1993).

    Article  CAS  Google Scholar 

  11. Li, S., Pathak, S. & Hsu, T.C. High resolution G-banding patterns of Syrian hamster chromosomes. Cytogenet. Cell Genet. 33, 295–302 (1982).

    Article  CAS  Google Scholar 

  12. Hirotsune, S. et al. Molecular cloning of polymorphic markers on RLGS gel using the spot target cloning method. Biochem. Biophys. Res. Commun. 194, 1406–1412 (1993).

    Article  CAS  Google Scholar 

  13. Ohsumi, T. et al. RLGS spot cloning method. Electrophoresis 16, 203–209 (1995).

    Article  CAS  Google Scholar 

  14. Matsuda, Y. et al. Location of the mouse complement factor H gene (chf) by FISH analysis and replication R-banding. Cytogenet. Cell Genet. 61, 282–285 (1992).

    Article  CAS  Google Scholar 

  15. Roberds, S.L. et al. Disruption of the dystrophin-glycoprotein complex in the cardiomyopathic hamster. J. Biol. Chem. 268, 11496–11499 (1993).

    CAS  PubMed  Google Scholar 

  16. Geisterfer-Lowrance, A.A.T. et al. A molecular basis for familial hypertrophic cardiomyopathy: a β cardiac myosin heavy chain gene missense mutation. Cell 62, 999–1006 (1990).

    Article  CAS  Google Scholar 

  17. Thierfelder, L. et al. α-Tropomyosin and cardiacv troponin T mutations cause familial hypertrophic cardiomyopathy: a disease of the sarcomere. Cell 77, 701–712 (1994).

    Article  Google Scholar 

  18. Okazaki, Y. et.al. Direct detection and isolation of RLGS spot DNA marker tightly linked to a specific trait using RLGS spot-bombing method. Proc. Natl. Acad. Sci. USA 92, 5610–5614 (1995).

    Article  CAS  Google Scholar 

  19. Hirotsune, S. et al. The reeler gene encodes a protein with an EGF-like motif expressed by pioneer neurons. Nature Genet. 10, 77–83 (1995).

    Article  CAS  Google Scholar 

  20. Sambrook, J., Fritsch, E.F. & Maniatis, T. Molecular cloning: A laboratory manual. 2nd edn. (Cold Spring Harbor Laboratory Press, New York, 1989).

    Google Scholar 

Download references

Author information

Author notes

  1. Yoichi Matsuda

    Present address: Laboratory of Animal Genetics, School of Agricultural Sciences, Nagoya University, Chikusa, Nagoya, 464-01, Japan

  2. Yasushi Okazaki and Hisato Okuizumi: Y.O. and H.O. contributed equally to this work.

Authors and Affiliations

  1. Genome Science Laboratory, Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), 3-1-1 Koyadai, Tsukuba, Ibaraki, 305, Japan

    Yasushi Okazaki, Hisato Okuizumi, Tomoya Ohsumi, Osamu Nomura, Shuji Takada, Mamoru Kamiya, Nobuya Sasaki, Masami Muramatsu & Yoshihide Hayashizaki

  2. Genome Research Group, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba, 263, Japan

    Yoichi Matsuda

  3. Institutefor Experimental Animals, Hamamatsu University School Medicine, 3600 Handa-cho, Hamamatsu, 431-31, Japan

    Masahiko Nishimura

  4. Department of Planning & Resources, Reseach Division, Eisai Company, 6-10, Koishikawa 4-chome, Bunkyo-ku, Tokyo, 112-88, Japan

    Osamu Tagaya

Authors
  1. Yasushi Okazaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hisato Okuizumi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tomoya Ohsumi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Osamu Nomura
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shuji Takada
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Mamoru Kamiya
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Nobuya Sasaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Yoichi Matsuda
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Masahiko Nishimura
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Osamu Tagaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Masami Muramatsu
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Yoshihide Hayashizaki
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Okazaki, Y., Okuizumi, H., Ohsumi, T. et al. A genetic linkage map of the Syrian hamster and localization of cardiomyopathy locus on chromosome 9qa2.1–b1 using RLGS spot–mapping. Nat Genet 13, 87–90 (1996). https://doi.org/10.1038/ng0596-87

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/ng0596-87

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing