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A manually curated functional annotation of the human X chromosome

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Figure 1: Identification of a new protein-coding gene, a transcribed pseudogene and a misannotated noncoding RNA.

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Correspondence to Akhilesh Pandey.

Supplementary information

Supplementary Fig. 1

Schematic outlining the strategy adopted for analysis of the X chromosome. (PDF 185 kb)

Supplementary Fig. 2

Density of protein-coding genes, pseudogenes and repeats on the X chromosome. (PDF 276 kb)

Supplementary Fig. 3

Agarose gel showing successful PCR amplification of a subset of novel genes. (PDF 609 kb)

Supplementary Fig. 4

Protein level alignment between human and mouse showing two conserved homology blocks that represent two coding exons of a novel gene. (PDF 176 kb)

Supplementary Fig. 5

Schematic representing novel coding exons identified upstream of a known gene. (PDF 281 kb)

Supplementary Fig. 6

Pie chart distribution of the pseudogenes. (PDF 149 kb)

Supplementary Fig. 7

Alignment of FUNDC2 encoded protein and conceptual translation of its pseudogene showing mutations and in frame stop codon. (PDF 257 kb)

Supplementary Fig. 8

Mapping of previously reported non-coding RNA transcripts to protein coding genes. (PDF 234 kb)

Supplementary Fig. 9

Pie charts depicting distribution of the X proteome according to molecular function, biological process and cellular component. (PDF 160 kb)

Supplementary Table 1

A comparison of features of the X chromosome with other human chromosomes analyzed to date. (PDF 29 kb)

Supplementary Table 2

A list of putative novel protein coding regions based on comparative genomics analysis. (PDF 19 kb)

Supplementary Table 3

A list of putative novel coding exons for known genes on X. (PDF 10 kb)

Supplementary Table 4

Statistics of the pseudogenes identified on the human X chromosome. (PDF 11 kb)

Supplementary Table 5

A list of all pseudogenes identified on the X chromosome. (PDF 58 kb)

Supplementary Table 6

Statistics pertaining to pseudogenes derived from known genes on X chromosome. (PDF 12 kb)

Supplementary Table 7

A list of all the pseudogenes derived from protein coding genes on human X chromosome. (PDF 40 kb)

Supplementary Table 8

A list of novel alternative splicing events discovered for the protein coding genes on X. (PDF 47 kb)

Supplementary Table 9

Analysis and mapping of the previously reported non-coding RNAs transcribed from X. (PDF 23 kb)

Supplementary Methods (PDF 15 kb)

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Harsha, H., Suresh, S., Amanchy, R. et al. A manually curated functional annotation of the human X chromosome. Nat Genet 37, 331–332 (2005). https://doi.org/10.1038/ng0405-331

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  • DOI: https://doi.org/10.1038/ng0405-331

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