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Construction by gene targeting in human cells of a ‘conditional’ CDC2 mutant that rereplicates its DNA

Nature Genetics volume 15, pages 258–265 (1997)Cite this article

Abstract

We describe a novel gene targeting strategy for the genetic analysis of essential genes in mammalian cells and its use to study the role of the cell cycle control gene CDC2 in human cells. A cell line (HT2-19) was generated in which endogenous CDC2 gene expression and cell viability depend on the presence of an inducer in the growth medium. In the absence of inducer, HT2-19 cells undergo extensive DNA rereplication and apoptosis. Rereplication is indicative of a role for human CDC2 in a control mechanism, previously undetected in mammalian cells, that prevents premature entry into S-phase.

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Authors and Affiliations

  1. MRC Clinical Sciences Centre, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London, W12 ONN, UK

    Jane E. Itzhaki & Andrew C.G. Porter

  2. Imperial Cancer Research, Fund Laboratories, 44 Lincoln's Inn Fields, London, WC2A 3PX, UK

    Christopher S. Gilbert

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Itzhaki, J., Gilbert, C. & Porter, A. Construction by gene targeting in human cells of a ‘conditional’ CDC2 mutant that rereplicates its DNA. Nat Genet 15, 258–265 (1997). https://doi.org/10.1038/ng0397-258

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