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Correcting CRISPR for copy number

The CRISPR–Cas9 system enables global screens of gene function with high sensitivity and specificity, but off-target effects have been reported for CRISPR guide RNAs targeting genes that are amplified at high copy number. A new study describes a computational approach to correct for this copy number effect, increasing the specificity of CRIPSR screens to identify essential genes.

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Author information

Affiliations

  1. John Paul Shen and Trey Ideker are in the Department of Medicine, University of California, San Diego, La Jolla, California, USA.

    • John Paul Shen
    •  & Trey Ideker

Authors

  1. Search for John Paul Shen in:

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Competing interests

T.I. is a cofounder of Data4Cure, Inc., and has equity interest. T.I. has an equity interest in Ideaya Biosciences, Inc. The terms of these arrangements have been reviewed and approved by the University of California, San Diego, in accordance with its conflict-of-interest policies.

Corresponding author

Correspondence to Trey Ideker.