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Correcting CRISPR for copy number

Nature Genetics volume 49, pages 1674–1675 (2017)Cite this article

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The CRISPR–Cas9 system enables global screens of gene function with high sensitivity and specificity, but off-target effects have been reported for CRISPR guide RNAs targeting genes that are amplified at high copy number. A new study describes a computational approach to correct for this copy number effect, increasing the specificity of CRIPSR screens to identify essential genes.

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Figure 1: Gene-independent copy number effects in CRISPR–Cas9 essentiality screens.

Katie Vicari/Springer Nature

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Authors and Affiliations

  1. the Department of Medicine, University of California, San Diego, La Jolla, California, USA

    John Paul Shen & Trey Ideker

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  1. John Paul Shen
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  2. Trey Ideker
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Correspondence to Trey Ideker.

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Competing interests

T.I. is a cofounder of Data4Cure, Inc., and has equity interest. T.I. has an equity interest in Ideaya Biosciences, Inc. The terms of these arrangements have been reviewed and approved by the University of California, San Diego, in accordance with its conflict-of-interest policies.

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Shen, J., Ideker, T. Correcting CRISPR for copy number. Nat Genet 49, 1674–1675 (2017). https://doi.org/10.1038/ng.3994

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