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Characterization of Murine Leukaemia Virus-specific DNA present in Normal Mouse Cells

Nature New Biology volume 244, pages 76–79 (1973)Cite this article

Abstract

RNA type C viruses have been isolated from many animal species. In the mouse and chicken, they exist in an unexpressed form in every cell1–3. Whether endogenous type C viral genetic information is integrated within the cell genome has been studied genetically and biochemically. Loci for inducibility of biologically distinguishable viruses have been detected in mouse cells, suggesting that the loci may represent virus structural information4,5. Numerous biochemical studies have characterized and quantified type C virus-specific DNA in avian and murine cells6–9. In some reports, the number of viral DNA equivalents per cell was quantified by measuring the effect of unlabelled cellular DNA on the reassociation kinetics of a labelled DNA probe10. Using labelled double stranded (ds) DNA prepared in vitro with the reverse transcriptases of RNA tumour viruses, earlier reports indicated that the major portion of the type C virus ds DNA product was complementary to a relatively small fraction of the viral genome7,11; this in vitro DNA probe did not effectively discriminate between the DNAs of different species with respect to the presence of viral genetic information7,8. A portion of the ds DNA product was also shown to have a genetic complexity more representative of the 70S genome7,11.

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Authors and Affiliations

  1. Laboratory of Biology of Viruses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, 20014

    LAWRENCE D. GELB, JULIE B. MILSTIEN & MALCOLM A. MARTIN

  2. Viral Carcinogenesis Branch, National Cancer Institute, Bethesda, Maryland, 20014

    STUART A. AARONSON

Authors
  1. LAWRENCE D. GELB
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  2. JULIE B. MILSTIEN
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  3. MALCOLM A. MARTIN
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  4. STUART A. AARONSON
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GELB, L., MILSTIEN, J., MARTIN, M. et al. Characterization of Murine Leukaemia Virus-specific DNA present in Normal Mouse Cells. Nature New Biology 244, 76–79 (1973). https://doi.org/10.1038/newbio244076a0

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