Abstract
IN contrast to adult frog haemoglobin of Rana catesbeiana, the tadpole haemoglobin has long been known to be one of the unusual haemoglobins with no Bohr effect. They are tetramers composed of two kinds of unlike chains1–5. We have demonstrated a substantial decrease in the oxygen affinity of the tadpole and the frog haemoglobins6, as influenced by organic phosphates, for example, 2,3-diphosphoglycerate (DPG), adenosine triphosphate (ATP) and inositol hexaphosphate (IHP), at pH 7.0. It has been reported that DPG additionally enhances the Bohr effect of mammalian haemoglobins7,8 and so we examined the effect of organic phosphates at various pH on tadpole and frog haemoglobins.
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ARAKI, T., KAJITA, A. & SHUKUYA, R. Latent Bohr Effect in the Tadpole Haemoglobin of Rana catesbeiana. Nature New Biology 242, 254–256 (1973). https://doi.org/10.1038/newbio242254a0
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DOI: https://doi.org/10.1038/newbio242254a0