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Production of a Nervous-System-Specific Protein (14-3-2) by Human Neuroblastoma Cells in Culture

Nature New Biology volume 241, pages 242–244 (1973)Cite this article

Abstract

CULTURED cell lines which continue to produce organ-specific products or exhibit differentiated physiological characteristics have been established from various neoplasms. Clonal cell lines which produce the nervous-system-specific protein S-1001,2 have been isolated from glial tumours3,4, and have provided information about the regulation of accumulation5 of S-100 and its synthesis and degradation6, as well as the regulation of several enzymes by hormones and neurotransmitters7. Clonal cell lines have also been derived from a spontaneous mouse neuroblastoma, C13008–10, many of them with the electrophysiological11–13, morphological14,15 and biochemical16–18 properties of neurones. Cell hybridization with C1300 clones has produced a tentative model for some aspects of neuronal differentiation19,20. Cell strains from human neuroblastomas21–23, however, have not been extensively exploited for physiological and biochemical studies. We report here the production of a nervous-system-specific protein thought to be localized primarily in neurones, 14-3-224, by the human neuroblastoma line IMR-3222.

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References

  1. Moore, B., and McGregor, D., J. Biol. Chem., 240, 1647 (1965).

    CAS  PubMed  Google Scholar 

  2. Moore, B., Biochem. Biophys. Res. Commun., 19, 739 (1965).

    Article  CAS  Google Scholar 

  3. Benda, P., Lightbody, L., Sato, G., Levine, L., and Sweet, W., Science, 161, 370 (1968).

    Article  CAS  Google Scholar 

  4. Lightbody, J., Pfeiffer, S., Kornblith, P., and Herschman, H., J. Neurobiol., 1, 411 (1970).

    Article  CAS  Google Scholar 

  5. Pfeiffer, S., Herschman, H., Lightbody, J., and Sato, G., J. Cell Physiol., 75, 329 (1970).

    Article  CAS  Google Scholar 

  6. Herschman, H., J. Biol. Chem., 246, 7569 (1971).

    CAS  PubMed  Google Scholar 

  7. de Vellis, J., Inglish, D., and Galey, F., in Cellular Aspects of Neural Growth and Differentiation (edit. by Pease, D.), 23 (University of California Press, Berkeley, 1971).

    Google Scholar 

  8. Augusti-Tocco, G., and Sato, G., Proc. US Nat. Acad. Sci., 64, 311 (1969).

    Article  CAS  Google Scholar 

  9. Klebe, R., and Ruddle, F., J. Cell Biol., 43, 69a (1969).

    Google Scholar 

  10. Schubert, D., Humphreys, S., Baroni, C., and Cohen, M., Proc. US Nat. Sci., 64, 316 (1969).

    Article  CAS  Google Scholar 

  11. Nelson, P., Ruffner, W., and Nirenberg, M., Proc. US Nat. Acad. Sci., 64, 1004 (1969).

    Article  CAS  Google Scholar 

  12. Harris, A., and Dennis, M., Science, 167, 1253 (1970).

    Article  CAS  Google Scholar 

  13. Harris, A., Heinemann, S., Schubert, D., and Tarikas, H., Nature, 231, 296 (1971).

    Article  CAS  Google Scholar 

  14. Seeds, N., Gilman, A., Amano, T., and Nirenberg, M., Proc. US Nat. Acad. Sci., 66, 160 (1970).

    Article  CAS  Google Scholar 

  15. Schubert, D., Humphreys, S., DeVitry, J., and Jacob, F., Develop. Biol., 25, 514 (1971).

    Article  CAS  Google Scholar 

  16. Blume, A., Gilbert, F., Wilson, S., Farber, J., Rosenberg, R., and Nirenberg, M., Proc. US Nat. Acad. Sci., 67, 786 (1970).

    Article  CAS  Google Scholar 

  17. Kates, J., Winterton, R., and Schlessinger, K., Nature, 229, 345 (1971).

    Article  CAS  Google Scholar 

  18. Dawson, G., Kemp, S., Stoolmiller, A., and Dorfman, A., Biochem. Biophys. Res. Commun., 44, 687 (1972).

    Article  Google Scholar 

  19. Minna, J., Nelson, P., Peacock, J., Glazer, D., and Nirenberg, M., Proc. US Nat. Acad. Sci., 68, 234 (1971).

    Article  CAS  Google Scholar 

  20. Minna, J., Glazer, D., and Nirenberg, M., Nature New Biology, 235, 225 (1972).

    Article  CAS  Google Scholar 

  21. Goldstein, M., Burdman, J., and Journey, L., J. Nat. Cancer Inst., 32, 165 (1964).

    CAS  PubMed  Google Scholar 

  22. Tumilowicz, J., Nichols, W., Cholon, J., and Green, A., Cancer Res., 30, 2110 (1970).

    CAS  PubMed  Google Scholar 

  23. Lyon, G., Cancer Res., 30, 2521 (1970).

    CAS  PubMed  Google Scholar 

  24. Perez, V. J., Olney, J. W., Cicero, T. S., Moore, B. W., and Bahn, B. A., J. Neurochem., 17, 511 (1970).

    Article  CAS  Google Scholar 

  25. Levine, L., in Handbook of Experimental Immunochemistry (edit. by Weir, D. M,), 707 (Blackwell Scientific Publications, Oxford, 1967).

    Google Scholar 

  26. Ham, R. G., Exp. Cell Res., 29, 515 (1963).

    Article  CAS  Google Scholar 

  27. Puck, T. T., Cieciura, S., and Fisher, H., J. Exp. Med., 106, 145 (1951)

    Article  Google Scholar 

  28. Lowry, O., Rosebrough, N., Farr, A., and Randall, R., J. Biol. Chem., 193, 265 (1961).

    Google Scholar 

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  1. Department of Biological Chemistry and Laboratory of Nuclear Medicine, School of Medicine, University of Californa, Los Angeles, California, 90024

    HARVEY R. HERSCHMAN & MICHAEL P. LERNER

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  1. HARVEY R. HERSCHMAN
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HERSCHMAN, H., LERNER, M. Production of a Nervous-System-Specific Protein (14-3-2) by Human Neuroblastoma Cells in Culture. Nature New Biology 241, 242–244 (1973). https://doi.org/10.1038/newbio241242a0

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