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Comparison of the Redox Assay for ACTH with Previous Assays

Nature New Biology volume 241, pages 84–85 (1973)Cite this article

Abstract

NO methods are at present available for the detection of subnormal levels of pituitary hormones in serum or plasma. Even the most sensitive bioassays, radioreceptor assays and radioimmunoassays can detect only picogram per ml. concentrations. A hew biological assay has been described recently capable of determining femtogram (10−15 g) amounts of ACTH in diluent1,2. This report concerns the validation of this assay in a comparative study with the Lipscomb–Nelson bioassay and two radioimmunoassays (directed against different parts of the ACTH molecule). Subnormal levels of circulating ACTH following the i.v. administration of Cortisol to a normal subject have been measured for the first time, and the half-time for disappearance of endogenous hormone calculated.

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Authors and Affiliations

  1. Department of Chemical Pathology and the Medical Professorial Unit, St Bartholomew's Hospital, London, ECI

    LESLEY H. REES, J. G. RATCLIFFE, G. M. BESSER, ROSANNE KRAMER & J. LANDON

  2. Division of Cellular Biology, The Mathilda and Terence Kennedy Institute of Rheumatology, London

    J. CHAYEN

Authors
  1. LESLEY H. REES
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  2. J. G. RATCLIFFE
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  3. G. M. BESSER
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  4. ROSANNE KRAMER
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  5. J. LANDON
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  6. J. CHAYEN
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REES, L., RATCLIFFE, J., BESSER, G. et al. Comparison of the Redox Assay for ACTH with Previous Assays. Nature New Biology 241, 84–85 (1973). https://doi.org/10.1038/newbio241084a0

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  • DOI: https://doi.org/10.1038/newbio241084a0

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