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Reverse Transcriptase Activity of Human Acute Leukaemic Cells: Purification of the Enzyme, Response to AMV 70S RNA, and Characterization of the DNA Product

Nature New Biology volume 240, pages 67–72 (1972)Cite this article

Abstract

A particulate fraction from human leukaemic blood cells contains an RNAase-sensitive endogenous DNA polymerase system. This DNA polymerase was purified, and it transcribes heteropolymeric regions of AMV 70S RNA. These and other template characteristics are similar to those of RNA tumour virus reverse transcriptase, and appear to distinguish this leukaemic DNA polymerase from other normal cellular DNA polymerases.

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Author notes

  1. ROBERT C. GALLO

    Present address: †Laboratory of Tumor Cell Biology f, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20014

  2. PREM S. SARIN

    Present address: Laboratory of Tumor Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20014

Authors and Affiliations

  1. Department of Molecular Biology, Bionetics Research Laboratories, Bethesda, Maryland, 20014

    M. G. SARNGADHARAN, PREM S. SARIN & MARVIN S. REITZ

  2. Laboratory of Tumor Cell Biology†, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20014

    ROBERT C. GALLO

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  1. M. G. SARNGADHARAN
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  3. MARVIN S. REITZ
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  4. ROBERT C. GALLO
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SARNGADHARAN, M., SARIN, P., REITZ, M. et al. Reverse Transcriptase Activity of Human Acute Leukaemic Cells: Purification of the Enzyme, Response to AMV 70S RNA, and Characterization of the DNA Product. Nature New Biology 240, 67–72 (1972). https://doi.org/10.1038/newbio240067a0

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  • DOI: https://doi.org/10.1038/newbio240067a0

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