Abstract
The relatively rapid loss of human chromosomes from human-rodent somatic cell hybrids has allowed the determination of linkage relationships between several human genes1–4. Cells that have segregated out most of the human chromosomes are analysed for the presence or absence of particular human gene products; when two gene products are always found to be retained together, they are assumed to be linked. Little has been done to extend these genetic techniques to cell hybrids formed between two different mutants of the same cell line. A linkage analysis would provide a valuable means of interpreting the gene function altered in such mutants. The principal obstacle to such an approach has been the fact that homospecific cell hybrids are rather stable, losing chromosomes at only a low rate5–7. Nevertheless, by using suitably marked strains, it is possible to select rare segregants from a homospecific hybrid population7,8. I have applied such a system to test for linkage between several chemically induced mutations in a Chinese hamster cell line.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
We are sorry, but there is no personal subscription option available for your country.
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Weiss, M. C., and Green, H., Proc. US Nat. Acad. Sci., 58, 1104 (1967).
Ruddle, F. H., Chapman, V. H., Ricciuti, F., Murname, M., Klebe, R., and Meera Khan, P., Nature New Biology, 232, 69 (1971).
Puck, T. T., Wuthier, P., Jones, C., and Kao, F. T., Proc. US Nat. Acad. Sci., 68, 3102 (1971).
Miller, O. J., Cook, P. R., Khan, P. M., Sin, S., and Siniscalco, M., Proc. US Nat. Acad. Sci., 68, 116 (1971).
Engel, E., McGee, B. J., and Harris, H., Nature, 223, 152 (1969).
Kao, F. T., Johnson, R. T., and Puck, T. T., Science, 164, 312 (1969).
Marin, G., Exp. Cell Res., 57, 29 (1969).
Westerveld, A., Visser, R. P. L. S., and Freeke, M. A., Biochem. Genet., 5, 541 (1971).
Murray, A. W., Elliott, D. C., and Atkinson, M. R., Prog. Nucleic Acid Res. Mol. Biol., 10, 87 (1970).
Kao, F. T., and Puck, T. T., Proc. US Nat. Acad. Sci., 60, 1275 (1968).
Ham, R. G., Proc. US Nat. Acad. Sci., 53, 288 (1965).
Kao, F. T., Chasin, L. A., and Puck, T. T., Proc. US Nat. Acad. Sci., 64, 1284 (1969).
Luria, S. E., and Delbrück, M., Genetics, 28, 491 (1943).
Kusano, T., Long, C., and Green, H., Proc. US Nat. Acad. Sci., 68, 82 (1971).
Pontecorvo, G., Nature, 230, 367 (1971).
Littlefield, J. W., Proc. US Nat. Acad. Sci., 50, 568 (1963).
Lowry, O. H., Roseborough, N. J., Farr, A. L., and Randall, R. J., J. Biol. Chem., 193, 265 (1951).
Lea, D. E., and Coulson, C. A., J. Genetics, 49, 264 (1949).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
CHASIN, L. Non-linkage of Induced Mutations in Chinese Hamster Cells. Nature New Biology 240, 50–52 (1972). https://doi.org/10.1038/newbio240050a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/newbio240050a0
This article is cited by
-
Diphtheria toxin resistance in chinese hamster cells: Genetic and biochemical characteristics of the mutants affected in protein synthesis
Somatic Cell Genetics (1980)
-
Random segregation of multiple genetic markers from CHO-CHO hybrids: Evidence for random distribution of functional hemizygosity in the genome
Somatic Cell Genetics (1980)
-
Analysis of steroid resistance in lymphoid cell hybrids
Somatic Cell Genetics (1980)
-
[3H]Amino acid selection of aminoacyl-tRNA synthetase mutants of CHO cells: Evidence of homo- vs. hemizygosity at specific loci
Somatic Cell Genetics (1979)
-
Evidence obtained by induced mutation frequency analysis for functional hemizygosity at theemt locus in CHO cells
Somatic Cell Genetics (1979)