Abstract
STUDY of effector-induced changes in enzyme activity has allowed identification of several mechanisms for control of gene expression in microorganisms1–3, and provided considerable evidence that the rate of transcription of specific genes may be governed by environmental conditions. By contrast, regulation by metabolites of enzyme activity in cultured mammalian diploid cells has been studied only rarely. We report here enhancement of galactokinase activity in cultured human fibroblasts by addition of substrate to the culture medium; lack of such induction in cells derived from a boy with galactokinase deficiency4, and the occurrence of a partial enzyme defect in the fibroblasts from his parents.
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ZACCHELLO, F., BENSON, P., BROWN, S. et al. Induction of Galactokinase in Fibroblasts from Heterozygous and Homozygous Subjects. Nature New Biology 239, 95–96 (1972). https://doi.org/10.1038/newbio239095a0
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DOI: https://doi.org/10.1038/newbio239095a0
