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Heterologous Antiserum to Thymus-derived Cells in the Guinea-pig

Nature New Biology volume 235, pages 19–21 (1972)Cite this article

Abstract

USEFUL antisera specific for thymocytes and peripheral T lymphocytes have only been widely available in the form of antisera to thymic isoantigens of the mouse1. We describe here the preparation and properties of a heterologous antiserum to guinea-pig thymocytes which is rendered specific for T lymphocytes after absorption with a pure population of B lymphocytes. We have already described2,3 the properties of the transplantable acute lymphatic leukaemia L2C of inbred strain two guinea-pigs. The L2C leukaemia cell is characterized as a B cell by the presence of surface immunoglobulin of the λ2 class, the secretion of a small amount of λ2 immunoglobulin, and the presence of a receptor for antigen-antibody-complement (C3) complexes characteristic of the B cell population4. Because, as will be shown, the antiserum is specific both for thymocytes and thymus derived lymphocytes, it will be referred to as anti-thymus derived cell (anti-TDC) serum. The availability of such an antiserum for a species in which the in vivo and in vitro manifestations of delayed hyper-sensitivity are so easily demonstrated may prove to be highly advantageous.

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Authors and Affiliations

  1. Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, 20014

    ETHAN SHEVACH, IRA GREEN & LEONARD ELLMAN

  2. Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York, 10461

    JEAN MAILLARD

Authors
  1. ETHAN SHEVACH
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  2. IRA GREEN
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  3. LEONARD ELLMAN
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  4. JEAN MAILLARD
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SHEVACH, E., GREEN, I., ELLMAN, L. et al. Heterologous Antiserum to Thymus-derived Cells in the Guinea-pig. Nature New Biology 235, 19–21 (1972). https://doi.org/10.1038/newbio235019a0

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