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Use of Antibody-coupled Cellulose as Immunosorbent in the Estimation of Human Immunoglobulin E (γE)

Nature New Biology volume 232, pages 245–247 (1971)Cite this article

Abstract

THE recent discoveries1, 2 of myeloma forms of IgE have made possible the development of a sensitive radio-immunosorbent method of estimating antibody IgE (reagin) in normal and pathological sera and other body fluids. The RIST (radioimmunosorbent technique3), as it has come to be known, is based on a method4 developed originally for the estimation of protein hormones. It offers a highly sensitive alternative to methods of assaying antibody (reaginic) IgE, being capable of detecting about 1,000 times less than the lower limit of immunoglobulin (1 µg) detectable by the single radial immunodiffusion technique5.

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Authors and Affiliations

  1. Department of Experimental Pathology, Department of Chemistry, University of Birmingham, Birmingham, 15

    P. MCLAUGHLAN & D. R. STANWORTH

  2. Department of Chemistry, University of Birmingham, Birmingham, 15

    J. F. KENNEDY & H. CHO TUN

Authors
  1. P. MCLAUGHLAN
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  2. D. R. STANWORTH
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  3. J. F. KENNEDY
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  4. H. CHO TUN
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MCLAUGHLAN, P., STANWORTH, D., KENNEDY, J. et al. Use of Antibody-coupled Cellulose as Immunosorbent in the Estimation of Human Immunoglobulin E (γE). Nature New Biology 232, 245–247 (1971). https://doi.org/10.1038/newbio232245a0

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