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Preservation of Functional Mammalian Polysomes by Lyophilization

Nature New Biology volume 230, pages 272–274 (1971)Cite this article

Abstract

LYOPHILIZATION has been used to preserve viable microorganisms for extended periods of time1. Recently, it has been reported that ribosomes functional in in vitro protein synthesis can be isolated from lyophilized fungi2 and that lyophilized Escherichia coliribosomes retain fully their capacity for poly U-directed phenylalanine incorporation after 5 months storage at room temperature over P2O5 (ref. 3). We have now compared the sedimentation profiles and poly U-directed phenylalanine incorporating activity of three types of rabbit reticulocyte ribosome preparations immediately after isolation and after freezing or lyophilization and storage for various times.

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References

  1. Martin, S. M., Ann. Rev. Microbiol., 12, 1 (1964).

    Article  Google Scholar 

  2. Leary, J. V., Morris, A. J., and Elingbore, A. H., Biochim. Biophys. Acta, 182, 113 (1969).

    Article  CAS  Google Scholar 

  3. Kato, T., and Tamaoki, T., Biochim. Biophys. Acta, 182, 250 (1969).

    Article  CAS  Google Scholar 

  4. Christman, J. K., and Goldstein, J., Biochim. Biophys. Acta (in the press).

  5. Lawford, G. R., Langford, P., and Schacter, H., J. Biol. Chem., 241, 1835 (1966).

    CAS  PubMed  Google Scholar 

  6. Lin, S., Mosteller, R. D., and Hardesty, B., J. Mol. Biol., 21, 51 (1966).

    Article  CAS  Google Scholar 

  7. Christman, J., and Goldstein, J., Biochim. Biophys. Acta, 179, 280 (1969).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. New York Blood Center, 310 East 67th Street, New York, NY 10021

    J. K. CHRISTMAN

  2. Department of Biochemistry, Cornell University Medical College, 1300 York Avenue, New York, NY 10021

    J. GOLDSTEIN

Authors
  1. J. K. CHRISTMAN
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  2. J. GOLDSTEIN
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CHRISTMAN, J., GOLDSTEIN, J. Preservation of Functional Mammalian Polysomes by Lyophilization. Nature New Biology 230, 272–274 (1971). https://doi.org/10.1038/newbio230272a0

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