Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Initiation of Reovirus mRNA Synthesis in vitro

Abstract

The ten mRNA species synthesized in vitro by reovirus-associated RNA polymerase contain the diphosphate, ppG, at the 5′-termini. The enzyme re-initiates continuously and digestion of the products with pancreatic RNAase releases predominantly ppGpUp from the 5′-ends.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Shatkin, A. J., Sipe, J. D., and Loh, P., J. Virology, 2, 986 (1968).

    CAS  PubMed  Google Scholar 

  2. Shatkin, A. J., and Sipe, J. D., Proc. US Nat. Acad. Sci., 61, 1462 (1968).

    Article  CAS  Google Scholar 

  3. Borsa, J., and Graham, A. F., Biochem. Biophys. Res. Commun., 33, 895 (1968).

    Article  CAS  Google Scholar 

  4. Skehel, J. J., and Joklik, W. K., Virology, 39, 822 (1969).

    Article  CAS  Google Scholar 

  5. Banerjee, A. K., and Shatkin, A. J., J. Virology, 6, 1 (1970).

    CAS  PubMed  Google Scholar 

  6. Levin, D. H., Mendelsohn, N., Schonberg, M., Klett, H., Silverstein, S., Kapuler, A. M., and Acs, G., Proc. US Nat. Acad. Sci., 66, 890 (1970).

    Article  CAS  Google Scholar 

  7. Shatkin, A. J., and Banerjee, A. K., Cold Spring Harbor Symp. Quant. Biol., 35 (1970).

  8. Levin, D. H., Acs, G., and Silverstein, S., Nature, 227, 603 (1970).

    Article  CAS  Google Scholar 

  9. Banerjee, A. K., Rensing, U., and August, J. T., J. Mol. Biol., 45, 181 (1969).

    Article  CAS  Google Scholar 

  10. Kapuler, A. M., Mendelsohn, N., Klett, H., and Acs, G., Nature, 225, 1209 (1970).

    Article  CAS  Google Scholar 

  11. Borsa, J., Grover, J., and Chapman, J. D., J. Virol., 6, 295 (1970).

    CAS  PubMed  PubMed Central  Google Scholar 

  12. Maitra, U., and Dubnoff, J., Fed. Proc., 26 (2), 349 (1967).

    Google Scholar 

  13. Roblin, R., J. Mol. Biol., 36, 125 (1968).

    Article  CAS  Google Scholar 

  14. Chamberlain, M., Cold Spring Harbor Symp. Quant. Biol., 35 (1970).

  15. Stavis, R. L., and August, J. T., Ann. Rev. Biochem., 39, 527 (1970).

    Article  CAS  Google Scholar 

  16. Millward, S., and Nonoyama, M., Cold Spring Harbor Symp. Quant. Biol., 35 (1970).

  17. Billeter, M. A., Dahlberg, J. E., Goodman, H. M., Hindley, J., and Weissmann, C., Nature, 224, 1083 (1969).

    Article  CAS  Google Scholar 

  18. Cory, S., and Adams, J. M., Cold Spring Harbor Symp. Quant. Biol., 35 (1970).

  19. Jeppesen, P. G. N., Nichols, J. L., Sanger, F., and Barrell, B. G., Cold Spring Harbor Symp. Quant. Biol., 35 (1970).

  20. Conway, T. W., and Lipmann, F., Proc. US Nat. Acad. Sci., 52, 1462 (1964).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

BANERJEE, A., WARD, R. & SHATKIN, A. Initiation of Reovirus mRNA Synthesis in vitro. Nature New Biology 230, 169–172 (1971). https://doi.org/10.1038/newbio230169a0

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1038/newbio230169a0

This article is cited by

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing