Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Regulation of lac Transcription in Antibiotic-treated E. coli

Nature New Biology volume 230, pages 41–44 (1971)Cite this article

Abstract

Chloramphenicol stabilizes pre-existing lac mRNA, but inhibits further accumulation by allowing rapid degradation of nascent message. Puromycin accelerates decay of pre-existing and new lac message by discharging protective ribo-somes. Both effects are partially reversed by the suA mutation.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Perlman, R. L., and Pastan, I., J. Biol. Chem., 243, 5420 (1968).

    CAS  PubMed  Google Scholar 

  2. Goldenbaum, P. E., and Dobrogosz, W. J., Biochem. Biophys. Res. Commun., 33, 828 (1968).

    Article  CAS  Google Scholar 

  3. Ullmann, A., and Monod, J., FEBS Lett., 2, 57 (1968).

    Article  CAS  Google Scholar 

  4. de Crombrugghe, B., Perlman, R. L., Varmus, H. E., and Pastan, I., J. Biol. Chem., 244, 5828 (1969).

    CAS  PubMed  Google Scholar 

  5. Pastan, I., and Perlman, R. L., Science, 169, 339 (1970).

    Article  CAS  Google Scholar 

  6. Varmus, H. E., Perlman, R. L., and Pastan, I., J. Biol. Chem., 245, 2259 (1970).

    CAS  Google Scholar 

  7. Varmus, H. E., Perlman, R. L., and Pastan, I., J. Biol. Chem. (in the press).

  8. de Crombrugghe, B., Varmus, H. E., Perlman, R. L., and Pastan, I., Biochem. Biophys. Res. Commun., 38, 894 (1970).

    Article  CAS  Google Scholar 

  9. Pastan, I., and Perlman, R. L., Proc. US Nat. Acad. Sci., 61, 1336 (1968).

    Article  CAS  Google Scholar 

  10. Perlman, R. L., de Crombrugghe, B., and Pastan, I., Nature, 223, 810 (1969).

    Article  CAS  Google Scholar 

  11. Silverstone, A. E., Magasanik, B., Reznikoff, W. S., Miller, J. H., and Beckwith, J. R., Nature, 221, 1012 (1969).

    Article  CAS  Google Scholar 

  12. Silverstone, A. E., Arditti, R., and Magasanik, B., Proc. US Nat. Acad. Sci., 66, 773 (1970).

    Article  CAS  Google Scholar 

  13. Pastan, I., and Perlman, R. L., J. Biol. Chem., 244, 2226 (1969).

    CAS  Google Scholar 

  14. Yudkin, M., and Moses, V., Biochem. J., 113, 423 (1969).

    Article  CAS  Google Scholar 

  15. Aboud, M., and Berger, M., Biochem. Biophys. Res. Commun., 38, 1023 (1970).

    Article  CAS  Google Scholar 

  16. Perlman, R. L., Chen, B., de Crombrugghe, B., Emmer, M., Gottesman, M., Varmus, H. E., and Pastan, I., Cold Spring Harbor Symp. Quant. Biol., 35 (in the press).

  17. Arditti, R. R., Eron, L., Zubay, G., Tocchini-Valentini, G., Connaway, S., and Beckwith, J. R., Cold Spring Harbor Symp. Quant. Biol., 35 (in the press).

  18. Beckwith, J., Biochim. Biophys. Acta, 76, 165 (1963).

    Article  Google Scholar 

  19. Morse, D. E., and Primakoff, P., Nature, 226, 28 (1970).

    Article  CAS  Google Scholar 

  20. Mehdi, Q., and Yudkin, M., Biochim. Biophys. Acta, 149, 288 (1967).

    Article  CAS  Google Scholar 

  21. Nakada, D., and Magasanik, B., J. Mol. Biol., 8, 105 (1964).

    Article  CAS  Google Scholar 

  22. Sypherd, P. S., and de Moss, J. A., Biochim. Biophys. Acta, 76, 589 (1963).

    Article  CAS  Google Scholar 

  23. Sypherd, P. S., and Strauss, N., Proc. US Nat. Acad. Sci., 49, 400 (1963).

    Article  CAS  Google Scholar 

  24. Morse, D. E., and Yanofsky, C., Proc. Intern. Lepetit Colloq., 1, 204 (1970).

    Google Scholar 

  25. Morse, D. E., J. Mol. Biol. (in the press).

  26. Morse, D. E., and Yanofsky, C., Nature, 224, 329 (1969).

    Article  CAS  Google Scholar 

  27. Yarmolinsky, M. B., and de la Haba, G. L., Proc. US Nat. Acad. Sci., 45, 1721 (1959).

    Article  CAS  Google Scholar 

  28. Allen, D. W., and Zamecnik, P. C., Biochem. Biophys. Acta, 55, 865 (1962).

    Article  CAS  Google Scholar 

  29. Nathans, D., Proc. US Nat. Acad. Sci., 51, 585 (1964).

    Article  CAS  Google Scholar 

  30. Smith, J. D., Traut, R. R., Blackburn, G. M., and Monro, R. E., J. Mol. Biol., 13, 617 (1965).

    Article  CAS  Google Scholar 

  31. Stubbs, J. D., and Hall, B. D., J. Mol. Biol., 73, 303 (1968).

    Article  Google Scholar 

  32. Cashel, M., J. Biol. Chem., 244, 3133 (1969).

    CAS  PubMed  Google Scholar 

  33. Levinthal, C., Fan, D. P., Higa, A., and Zimmerman, R. A., Cold Spring Harbor Symp. Quant. Biol., 28, 183 (1963).

    Article  CAS  Google Scholar 

  34. Morse, D. E., Mosteller, R. D., and Yanofsky, C., Cold Spring Harbor Symp. Quant. Biol., 34, 725 (1969).

    Article  CAS  Google Scholar 

  35. Fan, D. P., Higa, A., and Levinthal, C., J. Mol. Biol., 8, 210 (1964).

    Article  CAS  Google Scholar 

  36. Nakada, D., and Fan, D. P., J. Mol. Biol., 8, 223 (1964).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Clinical Endocrinology Branch, National Institute of Arthritis and Metabolic Diseases,

    H. E. VARMUS & R. L. PERLMAN

  2. Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20014

    I. PASTAN

Authors
  1. H. E. VARMUS
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. L. PERLMAN
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. I. PASTAN
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

VARMUS, H., PERLMAN, R. & PASTAN, I. Regulation of lac Transcription in Antibiotic-treated E. coli. Nature New Biology 230, 41–44 (1971). https://doi.org/10.1038/newbio230041a0

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1038/newbio230041a0

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing