Abstract
A system has been developed to study the regulation of the lactose operon in vitro. Transcription requires cyclic AMP and cyclic AMP binding protein and is repressed by lactose repressor protein.
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References
Jacob, F., and Monod, J., J. Mol. Biol., 3, 318 (1961).
Gilbert, W., and Muller-Hill, B., Proc. US Nat. Acad. Sci., 58, 2415 (1967).
Riggs, A. D., and Bourgeois, S., J. Mol. Biol., 34, 361 (1968).
Emmer, M., de Crombrugghe, B., Pastan, I., and Perlman, R. L., Proc. US Nat. Acad. Sci., 66, 480 (1970).
Zubay, G., Schwartz, D., and Beckwith, J. R., Proc. US Nat. Acad. Sci., 66, 104 (1970).
de Crombrugghe, B., Perlman, R. L., Varmus, H. E., and Pastan, I., J. Biol. Chem., 244, 5829 (1969).
Varmus, H. E., Perlman, R. L., and Pastan, I., J. Biol. Chem., 245, 2259 (1970).
de Crombrugghe, B., Varmus, H. E., Perlman, R. L., and Pastan, I., Biochem. Biophys. Res. Commun., 38, 894 (1970).
Perlman, R. L., Chen, B., de Crombrugghe, B., Emmer, M., Gottesman, M., Varmus, H. E., and Pastan, I., Cold Spring Harbor Symp. Quant. Biol., 35 (in the press).
Zubay, G., and Lederman, M., Proc. US Nat. Acad. Sci., 62, 550 (1969).
Chambers, D. A., and Zubay, G., Proc. US Nat. Acad. Sci, 63, 118 (1969).
Gillespie, D., and Spiegelman, S., J. Mol. Biol., 12, 829 (1965).
Varmus, H. E., Perlman, R. L., and Pastan, I., J. Biol. Chem. (in the press).
Ohshima, Y., Horiuchi, T., Iida, Y., and Kameyama, T., Mol. Gen., 106, 307 (1970).
Arditti, R. R., Eron, L., Zubay, G., Tocchini-Valentini, G., Connaway, S., and Beckwith, J. R., Cold Spring Harbor. Symp. Quant. Biol., 35 (in the press).
Zubay, G., cited in reference 17.
Cashel, M., and Gallant, J., Nature, 221, 838 (1969).
Stent, G. S., and Brenner, S., Proc. US Nat. Acad. Sci., 47, 2005 (1961).
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de CROMBRUGGHE, B., CHEN, B., GOTTESMAN, M. et al. Regulation of lac mRNA Synthesis in a Soluble Cell-free System. Nature New Biology 230, 37–40 (1971). https://doi.org/10.1038/newbio230037a0
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DOI: https://doi.org/10.1038/newbio230037a0
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