Glacial episodes have been linked to Ordovician–Silurian extinction events, but cooling itself may not be solely responsible for these extinctions. Teratological (malformed) assemblages of fossil plankton that correlate precisely with the extinction events can help identify alternate drivers of extinction. Here we show that metal poisoning may have caused these aberrant morphologies during a late Silurian (Pridoli) event. Malformations coincide with a dramatic increase of metals (Fe, Mo, Pb, Mn and As) in the fossils and their host rocks. Metallic toxins are known to cause a teratological response in modern organisms, which is now routinely used as a proxy to assess oceanic metal contamination. Similarly, our study identifies metal-induced teratology as a deep-time, palaeobiological monitor of palaeo-ocean chemistry. The redox-sensitive character of enriched metals supports emerging ‘oceanic anoxic event’ models. Our data suggest that spreading anoxia and redox cycling of harmful metals was a contributing kill mechanism during these devastating Ordovician–Silurian palaeobiological events.
Ordovician–Silurian rocks record several dramatic, positive stable isotope excursions indicative of major disturbances of the oceanic carbon, oxygen and sulfur cycles1,2. The environmental changes linked to these disturbances had a devastating effect on marine life. One of these events (the end-Ordovician Hirnantian event) features among the ‘Big Five’ Phanerozoic mass-extinction episodes3. Several other Ordovician–Silurian events are also marked by substantially elevated extinction rates among certain groups of marine taxa4,5,6, including the decimation of up to 95% of graptolite species during the Mulde event5 and 80% of conodonts during the Ireviken event4, along with losses in trilobites, brachiopods, corals, acritarchs, graptolites and chitinozoans. The Pridoli event that is studied here mainly affected corals, chitinozoans, graptolites and conodonts. Current causative models often revolve around the direct effects of changing palaeoclimate, such as cooling temperatures and habitat reduction, but do not fully integrate observed palaeontological, geological and geochemical shifts during these events7,8. Rooted in earlier models9, emerging geochemical and sedimentological evidence suggests that some of these disturbances are more directly linked to global oceanic anoxic events (OAEs) instead10,11,12. With the exception of some obvious links such as the drawdown of marine oxygen levels, however, the full set of interactions between OAEs originating in deep oceanic waters and mechanisms leading to extinctions across groups and palaeo-ecological niches on the continental shelves is still poorly understood. Elevated abundances of malformed (teratological) fossil microplankton (acritarchs and chitinozoans) mark the onset of the global, positive δ13Ccarb excursions13, and coincide with the initiation of extinction14.
Whereas modern instances of teratology in aquatic micro- and macroorganisms have been linked to a variety of environmental stressors, the teratological effects of heavy metal pollution have been well established in the natural environment and laboratory experiments. Malformation in diatoms15,16, foraminifera17,18, flagellates19, echinoderms20, arthropods21 and fish22,23 is now routinely used as a powerful forensic tool to identify contamination of fresh, brackish and marine waters. Biomonitoring of ecotoxicity using eggs (for example, of copepods or cladocerans) typically focuses on hatching success and viability of embryos and neonates24. Nevertheless, malformation has also been observed in copepod egg25 and chrysophyte algae cyst morphologies26, respectively, triggered by diatom-enriched diets of the parent copepods, and Mn–Cd metal pollution of the environment of the algae that originally produced the cysts. In terms of affected functional morphology, these modern case studies involving cysts and eggs are strikingly similar to those of chitinozoans and acritarchs in the early Palaeozoic record. In the modern, such studies of resting stages are uncommon because the producing organism can be studied directly. As soft-bodied parent organisms are usually not preserved in the fossil record, we are obliged to use readily preserved egg cases or cysts to reconstruct the original oceanic conditions.
Here, in analogy with the modern, we test whether palaeo pollution of the marine environment by toxic metals might have also caused the Silurian teratologies. We explore the geochemical signal of chitinozoan assemblages at one of the events with elevated abundances of malformed specimens near the end of the Silurian. Chitinozoans are organic-walled microfossils (palynomorphs), 100–500 μm long, which probably represent fossil egg cases of marine zooplankton that lived in the shallow mixed layer of the Ordovician–Silurian oceans27. We show that their teratology coincides with metal enrichment in host rocks and fossils.
Malformed acritarchs (cysts of marine phytoplankton28) can be abundant during these ancient events. For instance, there are up to 20% of teratological specimens in the affected assemblages from the Hirnantian of Anticosti Island13,14. The documented proportions of malformed chitinozoans are much lower: assemblages with c. 0.1% of affected specimens are considered anomalous, markedly above estimated background values of 0.01–0.001% (ref. 29). Nevertheless, we focused on the analysis of chitinozoans (Fig. 1) because they are an order of magnitude larger in size than acritarchs and thus easier to manipulate and analyse. We apply a new combination of techniques to measure the metal content of individual palynomorph specimens from teratological assemblages using time-of-flight secondary ion mass spectrometry (ToF-SIMS) and the bulk rock that hosts them, using laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS; see Methods). We selected a mid-Pridoli (late Silurian) event for which a teratology anomaly in the palynomorphs has been well established29,30. The samples are from a well in the Libyan desert that cuts through the Mesozoic and Palaeozoic sequence of the Gondwanan platform (Fig. 2). The studied interval consists of a sequence of regularly alternating siltstones and shales, with thin oolithic-ferruginous beds near the top of the section. Samples were collected from a series of cored intervals (Fig. 2) that yield abundant, exquisitely preserved palynomorphs. In core 14, teratological assemblages of chitinozoans29 and acritarchs30 of mid-Pridoli age (Margachitina elegans biozone31) have been previously described, respectively, between 2,127.5 and 2,125 m, and 2,129.6 and 2,126.4 m. This narrow cored interval yielded multiple samples and assemblages where the frequency of teratological chitinozoan specimens was one or two orders of magnitude above background estimates29. Published counts exceed 0.1% of malformed specimens and even up to 1.3% of affected ancyrochitinids29. Many additional specimens have been recovered from the same levels in the current study (Supplementary Dataset 1). Any taphonomic causes for the teratological morphologies have been excluded beyond reasonable doubt29,30. The morphological deformities consist of significant defects, including wild growth of tissue, and the uncontrolled twinning of specimens (Fig. 1a), which are clearly outside of the range of intraspecific variability. These cannot be confused with regularly occurring ecophenotypes of the same species, which display a continuous spectrum of much more subtle morphological variability linked to the changes in ecology, as for instance documented in the length of dinocyst spines that varies with salinity32.
The metal content, particularly Fe, Cu, As, Al, Pb, Ba, Mo and Mn, of individual chitinozoans and/or bulk-rock samples increases markedly in the teratological interval (Fig. 3a). Fe, As and Mn concentrations in bulk-rock samples increase by an order of magnitude, for example, from c. 50,000 to c. 400,000 p.p.m. Fe, on cue with the appearance of oolitic-ferruginous beds in the stratigraphic section (Fig. 3b). Principal component analysis on the ToF-SIMS data clearly separates the lower samples from those of the teratological interval; this is due to elevated metal content of the chitinozoan specimens in assemblages with malformation, notably of Fe, Al, Mo and Ba (Fig. 4a). While the metal enrichment in the teratology horizon is evident both in individual chitinozoans and in bulk-rock chemistry, abundances of certain elements in bulk-rock samples are decoupled from those in the palynomorphs: for example, bulk-rock Ba contents are relatively constant, yet the chitinozoan Ba shows a significant increase in the teratological zone (Fig. 3a). In addition, at decimetre scale and notably within the horizon with malformations, specimen and bulk-rock chemistry is statistically decoupled for all elements as evidenced by time-series cross-correlation tests (Figs 3b and 4b). This decoupling demonstrates that the chemistry of chitinozoans is not a mere imprint of rock composition but may preserve the original chemistry of the organisms and thus reflect metal abundances in their aquatic environment. Relying exclusively on the chemical signature of the chitinozoans to represent original ocean chemistry may be subject to debate. However, the coincidence between malformations, a primary biological feature developed in vivo, and the increased metal contents in the organisms and sediments, in combination, is best explained by an increase in dissolved metals in the water column during the life of the organism and deposition of the sediments. We conclude that metal pollution, that is, the uptake of those metals during life and/or harmful conditions that developed in tandem with a rising metal content of the seawater, is the most likely explanation for the increase of teratology observed in these fossils.
The mid-Pridoli teratology event correlates with faunal turnover33 and a positive δ13C excursion34 (Fig. 5). Early Palaeozoic bioevents were generally accompanied by large, positive δ13Ccarb excursions. Consensus is converging that such signatures may reflect the burial and sequestration of isotopically light carbon on the deep seafloor during developing OAEs35. It is also well established that transitions to anoxic conditions in modern marine environments increase markedly the solubility of some metals in seawater36. Most significantly, suboxic conditions lead to the reductive dissolution of Fe–Mn oxyhydroxides, increasing the concentration of Fe and Mn in modern seawater by orders of magnitude. The concentration of some trace elements such as As, Mo and rare earth elements are directly controlled by absorptive scavenging and co-deposition with these oxyhydroxides; and as a consequence of reductive dissolution of oxyhydroxides, increase significantly in concentration in the seawater37,38,39. Moreover, transitions from oxic to anoxic oceanic conditions can trigger massive shifts in the cycling of Fe–Mn oxyhydroxides sequestered in sub-seafloor sediments, thereby triggering a benthic flux of Fe, Mn and associated trace elements from the sediments into the anoxic seawater37,38,40. Other elements, such as Ba, are governed by linked processes such as the reduction of sulfate in anoxic sediments41. We posit that the observed metal enrichments and teratology during this Pridoli event reflects the encroachment of metal-enriched, oxygen-depleted oceanic waters into the oxic environments of the continental shelves. As this redox front moved into shallow water, the toxic mix of elements was introduced into the shelf ecosystems teeming with Silurian life. Consequently, the metals were re-oxidized and precipitated, each at their own pace (Supplementary Dataset 1), at the redox interface between the anoxic water mass and local oxic surface waters. This scenario accounts for the accumulation of redox-sensitive metals in both the sediments and fossils and explains the teratology of organisms during the Pridoli event. This interpretation is supported by similar enrichments of redox-sensitive metals through the early Wenlock Ireviken event12, by the enrichment of rare earth elements in widespread phosphorites across Laurentia42, and by the concurrence of ironstones with Silurian C-isotope excursions in the Appalachian Basin (USA). The US sections display consistent patterns of red, green and black marine sedimentary rocks, indicative of increasingly anoxic conditions10. In this model, the harmful effects of spreading dead zones43, decreasing oxygen and increasing metal concentrations are intricately entangled. For instance, a study44 of the seasonally anoxic Chesapeake Bay links the sudden occurrence of malformed foraminifer Ammonia to the onset of anoxic conditions in the 1970s. However, it is also known that these seasonal anoxic conditions triggered the prompt release of redox-sensitive metals from the sediments into the water column40. In our Silurian data, metal-enriched malformed assemblages occur at the onset of the carbon isotope excursions, while hypoxia was starting to spread (and before it peaks later, indicated by the deposition of black shales10). This suggests that a direct toxic effect of metal enrichment contributed to the most plausible mechanism for the deformities in the organisms. Other environmental stressors that are known to cause malformation in the modern include changes in light intensity, ultraviolet radiation, predation, salinity and pH, and could have coincided with spreading anoxic waters, but there is no empirical evidence for such changes in the event interval.
The metal composition of marine sedimentary rocks is a fundamental parameter used to interpret the chemical evolution of the Earth’s atmosphere and hydrosphere. Yet, generally, uncertainty remains regarding the primary metal abundances in seawater, pathways of metal accumulation and post-depositional modification of metal abundances in sedimentary rocks. As demonstrated in modern marine environments, our results suggest that metal-induced teratology of fossil plankton may serve as an independent proxy for monitoring changes in the metal concentrations of the shallow palaeo-ocean. This new proxy enables us here to reduce these uncertainties and supports the interpretation that the sedimentary chemistry reflects changing oceanic metal compositions during OAEs. As such, this proxy has the potential to help unravel the complexities inherent to sedimentary geochemistry, and may be a tool to evaluate other instances of marine metal variation through the geological record.
The co-occurrence between mid-Pridoli teratology and the onset of extinction suggests that metal contamination may have played a direct role in the biological crisis at large. More generally, the recurring temporal match between Ordovician–Silurian teratology events and extinction events13 raises the prospect that toxic metal contamination may be a previously unrecognized contributing agent to many, if not all, of these bioevents (Fig. 5). In sections where such data exist, teratological phytoplankton precede or co-occur with the earliest phases of these major extinction events. Taken at face value, for example, Hirnantian acritarch malformation in the Lousy Cove Member (member 6) of the Ellis Bay Formation coincides with the onset of the ‘phytoplankton crisis’ on Anticosti Island (Canada) but precedes the major macrofauna extinctions in the overlying Laframboise Member (member 7) (refs 14, 45). On Gotland (Sweden), increased acritarch malformation occurs around a series of marker beds46, identifying an interval that starts below the strongest extinctions of conodonts during the early Ireviken event4. Although the patterns and exact relative timing of teratology and extinction require confirmation, the available data suggest that malformed palynomorphs could be the proverbial ‘canaries in the coal mines’, that is, the first indicators of Palaeozoic extinctions.
Metal toxicity, and its fossilized in vivo expressions, could provide the ‘missing link’ between extinction of faunas on the shelf, and widespread ocean anoxia. As part of a series of complex systemic interactions accompanying these shifts in ocean conditions, the redox cycling of metals may identify the early phase of the kill mechanisms that culminated in these catastrophic events. Although OAEs typically entail rapid climatic change, our data suggest that the proposed mechanisms of these early Palaeozoic mass extinctions were previously too simply linked to global cooling, invoking thermal stress and habitat reduction. Ultimately, metal-induced teratology might become a new forensic tool to identify original oceanic geochemical signatures and may help unravel the biologic and geochemical systematics that define these extraordinary periods of Earth history.
Samples were studied from the 3,176 m A1-61 borehole in Ral-el-Dorah in Libya that cuts through the Mesozoic, Devonian, Silurian and into the Upper Ordovician sequence. The samples were collected from infrequently cored Silurian intervals. The original biostratigraphic purpose of the well explains why not every iron oolite bed in core 14 was sampled systematically, as oolites do not normally yield age-diagnostic microfossils (Fig. 2). Our samples 2,127 and 2,125.1 are direct subsamples from those of Jaglin and Paris29. Note that the teratological interval for chitinozoans in core 14 has been extended downwards by 0.5 m (Fig. 2) compared with published range29 as we found additional malformed specimens at 2,127.5 m (Supplementary Dataset 1).
Thirteen samples from A1-61 were dissolved for palynology. The core chips were broken into 0.5-cm pieces and decarbonated using 34% HCl. They were then agitated with c. 200 ml 48% HF over c. 24 h. Any newly formed fluor silicates were removed using a second 17% HCl treatment, over 32 h and at 60 °C. Samples were neutralized and filtered at 51 μm. Individual palynomorphs were handpicked from the organic residue (>51 μm) using a stereomicroscope at × 50–150 magnification, and transferred onto glass slides with Cu-tape and microscope grids, which were then used in ToF-SIMS analyses. Scanning electron microscope positioning of specimens versus reference grid was used to identify the specimens in the ToF-SIMS.
Time-of-flight secondary ion mass spectrometry
Individual microfossils from 13 samples from A1-61 were analysed using ToF-SIMS. ToF-SIMS spectra measurements were carried out using a TOF.SIMS 5 instrument (ION-TOF GmbH, Germany). This instrument is equipped with a Bi liquid metal ion gun. Pulsed Bi3+ primary ions have been predominantly used for analysis (25 keV, 0.3 pA). Analyses were performed semi-quantitatively using two settings: ‘mapping mode’ (500 × 500-μm2 grids, and the acquired images were 512 × 512 pixels, which has the advantage of simultaneously analysing multiple specimens) and ‘focused beam mode’ (focussing the Bi-pulsed beam on a 5 × 5-μm2 grid and in doing so removing surface layers from specimens). Both ‘mapping’ and ‘focused beam’ acquisitions were performed in high-current bunched mode. The primary Bi3+ ion current measured in the Faraday cup was 0.4 pA. In the mapping-mode experimental conditions, we obtained a lateral resolution of c. 1 μm. Mass resolution calculated at m/z=56 for Fe+ was >4,000. The primary ion dose density (PIDD, calculated after 10 shots per pixel) was 3 × 1011 ions per cm2, indicative of static analytical conditions. In the focused beam mode, the mass resolution calculated at m/z=56 for Fe+ remained >4,000. The primary ion dose density was 2 × 1015 ions per cm2 (non-static). The focused beam mode allows for a substantial reduction of modern contaminants (for example, PDMS, see Supplementary Fig. 1). The data from the 170 focused beam analyses (including 141 on chitinozoans) are those presented in the main paper (Supplementary Datasets 1 and 2). Measurements were accumulated over 133–200 s. The mapping-mode data are deposited as Supplementary Datasets 1 and 2, and mirror the trends of the focused beam-mode data. These ToF-SIMS maps also illustrate a uniform distribution of the key elements through the palynomorphs, excluding mineral origin (Supplementary Fig. 2).
Owing to the exploratory nature of study, the mapping-mode analyses used two different source modes, Bi+ and Bi3+. These are clearly separated in the Supplementary Dataset 1. For the ‘focused beam-mode’ analyses, only the Bi3+ source was used (this is the data in Figs 3 and 4). The data from the ‘Bi3+ mapping mode’ clearly displays the same trend as for the ‘focused beam’ mode, that is, a clear increase in metals in the samples with teratological assemblages, compared with the other samples lower in the section (this is the bulk of the samples in Supplementary Dataset 1). For the ‘Bi+ mapping mode’, this is somewhat less obvious (only three samples were analysed due to a markedly weaker signal), however, increases are observed for elements such as Fe, Al and Mo, in the chitinozoans of the two teratological samples analysed in Bi+ mode.
Sixty-six ion peaks were originally selected in the spectra. Twenty-one remained after eliminating those with potential overlap (for example, between peaks of Mn and 54FeH+, Ga+ and CHFe+) and those below the background noise threshold. We retained data for 19 ions (excluding H+ and C+) from a total number of 534 specimens of chitinozoans, acritarchs, scolecodonts (jaws of polychaete annelids), spores and graptolite fragments (organic-walled macro-zooplankton) that were analysed in mapping mode (Supplementary Dataset 2); a further 170 analyses, focussing on chitinozoans, were conducted in focused beam mode.
Laser ablation-inductively coupled plasma-mass spectrometry
Major and trace element abundances for 32 bulk-sediment samples were determined by LA-ICP-MS at the USGS (Supplementary Dataset 3). Analyses are within 15% of accepted values of USGS reference materials, duplicate analyses of selected samples by inductively coupled plasma-atomic emission spectrometry and inductively coupled plasma-mass spectrometry (ICP-AES-MS) sodium peroxide sinter (SGS Mineral Services, Toronto) and in-house ICP-AES-MS sinter methods. Replicate analyses were <3% RSD.
Chemical measurements of whole rocks and fossils were partitioned into 20-cm regular-spaced intervals between 2,125.1 and 2,459 m. Chemical measurements of each element were averaged within each interval and empty intervals (no measurements) were omitted from each time series. Averaged values were standardized to unit variance and a mean of zero before performing cross-correlation tests. Reported correlation coefficients are without temporal lag and non-parametric (Spearman’s Rho).
How to cite this article: Vandenbroucke, T. R. A. et al. Metal-induced malformations in early Palaeozoic plankton are harbingers of mass extinction. Nat. Commun. 6:7966 doi: 10.1038/ncomms8966 (2015).
T.R.A.V., M.Q. and K.L. acknowledge the financial support from the CNRS (INSU, action INTERRVIE), and the Regional Council of Nord-Pas de Calais (emergent projects action) in France. T.R.A.V. and T.S. acknowledge the financial support from the ANR through grant 12-BS06-0014 ‘SeqStrat-Ice’. N.N. acknowledges funding from the Chevreul institute (FR 2638), the European Regional Development Fund (FEDER), CNRS, the Regional Council of Nord-Pas de Calais and Ministère de l’Education Nationale de l’Enseignement Supérieur et de la Recherche for the ToF-SIMS spectrometer at the Pôle Régional d’Analyses de Surface. Laurence Debeauvais and Philippe Recourt (both CNRS, Univ. Lille) are thanked for the assistance with palynology lab protocols and scanning electron microscope imaging, respectively. Marco Vecoli (Saudi Aramco) is acknowledged for providing access to samples. This is a contribution to IGCP 591 ‘The early to middle Palaeozoic revolution’. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government.
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/