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# Visualizing active membrane protein complexes by electron cryotomography

• Nature Communications 5, Article number: 4129 (2014)
• doi:10.1038/ncomms5129
Accepted:
Published online:

## Abstract

Unravelling the structural organization of membrane protein machines in their active state and native lipid environment is a major challenge in modern cell biology research. Here we develop the STAMP (Specifically TArgeted Membrane nanoParticle) technique as a strategy to localize protein complexes in situ by electron cryotomography (cryo-ET). STAMP selects active membrane protein complexes and marks them with quantum dots. Taking advantage of new electron detector technology that is currently revolutionizing cryotomography in terms of achievable resolution, this approach enables us to visualize the three-dimensional distribution and organization of protein import sites in mitochondria. We show that import sites cluster together in the vicinity of crista membranes, and we reveal unique details of the mitochondrial protein import machinery in action. STAMP can be used as a tool for site-specific labelling of a multitude of membrane proteins by cryo-ET in the future.

## Introduction

The structural and functional organization of biological membranes and their resident protein complexes are of central importance in biomedical research. To date, few tools are available to identify molecular machines within their native lipid environment and to study their dynamic distribution on the nanometre scale. Electron cryotomography (cryo-ET) bridges the gap between light microscopy, which provides low-resolution information and high-resolution protein structures determined by X-ray crystallography. One advantage of cryo-ET over conventional electron microscopy (EM) is the ability to visualize proteins within whole cells or organelles (<500 nm), without staining or dehydration of the sample, reducing the risk of artefacts. However, it is a major challenge to localize specific cellular components in the complex overcrowded milieu of the cell.

In principle, clonable heavy metal tags such as bacterial ferritin1 or concatenated metallothioneins2 can be used as labels in cryo-ET. Ferritin has a dense iron core (capable of binding up to 4,500 Fe(III) atoms)3 and low cytotoxicity, and is visible in tomograms as a large ~450 kDa multisubunit assembly1. However, because it is an oligomer, multiple copies of a cloned target protein assemble onto one ferritin complex1, which carries the risk of mislocalization and likely restricts its use to soluble proteins. Metallothioneins are heavy metal binding proteins of ~6 kDa, and may be used in tandem copies if required. The heavy metal clusters (for example, Pt, Au, Cd) are small (typically <40 metal atoms per metallothionein)4 and therefore, more difficult to detect in noisy tomographic volumes. Their use has so far been limited to well-characterized soluble proteins, which are present in high copy numbers5,6, so that the extra density provided by the metal cluster is easily apparent. It seems unlikely that metallothionein tags will be suitable for many in vivo studies, as the production of the protein is dependent on growth of cells in high concentrations of reactive and often cytotoxic heavy metal ions. Such tags are likely to prove their worth in identification of oligomers, or of subunits within large complexes after subtomogram averaging.

Other clonable tags with potential importance for correlative light and EM include the fluorescent labels FlAsH and ReAsH7,8. The site of the fluorescent signal is seen in the electron microscope as a photo-oxidized precipitate. However, cells must be treated with antidotes to prevent cytotoxicity8, and moreover in electron cryomicroscopy (cryo-EM), interesting structural detail would be obscured by the large size of the deposit9.

The discrimination between active and inactive populations of membrane protein complexes by cryo-ET has been largely intractable. Recent advances in correlative light and EM7, such as the development of quantum dots (QDs), have opened up new perspectives to overcome these problems. QDs are brightly fluorescent and directly visible in the electron microscope due to their high electron scattering potential, containing between 100 and 100,000 Cd and Se atoms10. However, the few published cryo-EM studies have relied on antibody-QD labelling in fixed and stained material11,12, which limits resolution and identification of proteins.

Here we describe a novel approach to labelling for cryo-ET, called STAMP (Specifically TArgeted Membrane nanoParticle), which overcomes many of the drawbacks associated with currently available heavy metal tags. To demonstrate this, we compare our labelling method with the previously reported metallothionein technology2,5,6. We have used STAMP to visualize a membrane-bound molecular machine in action, without the need for compromising fixatives or stains. In order to specifically select for the active complexes, we use a three-step labelling process: (1) production of a small, tagged ligand; (2) specific targeting to active membrane complexes; (3) attachment of dense QD nanoparticles.

Mitochondria have an outer and an inner membrane and contain at least 1,000 different proteins synthesized in the cytosol. The majority of these proteins contain amino-terminal targeting signals (presequences) and are destined for the mitochondrial inner membrane or matrix. Concomitant import of these preproteins by the general translocase of the outer membrane (TOM complex) and the presequence translocase of the inner membrane (TIM23 complex) leads to the formation of translocation intermediates that span both membranes13,14,15. The analysis of these translocation contact sites between mitochondrial outer and inner membranes has been difficult, as their visualization has so far only been possible at low resolution in chemically fixed and sectioned samples16,17. Thus, only limited information is available on the structural organization and spatial distribution of these intricate protein-trafficking molecular hubs.

Using STAMP, the three-dimensional distribution and organization of protein import sites in mitochondria is demonstrated. Importing protein complexes are located in discrete clusters, close to, but not directly at crista junctions (CJs). Detailed analysis of the preprotein import machinery in action reveals that the TOM–TIM23 supercomplex is tethered by protein–protein interactions and does not involve close membrane contacts16,17.

## Results

### Labelled proteins arrest as membrane spanning intermediates

We devised a protocol for the site-specific QD labelling of a recombinant model preprotein that can be quantitatively arrested as a two-membrane spanning translocation intermediate (Fig. 1a). Model preproteins consisting of N-terminal parts of cytochrome b2 fused to dihydrofolate reductase (DHFR) have been widely used for biochemical studies on the import reaction. We generated a variant of cytochrome b2Δ-DHFR18 containing a unique carboxy-terminal cysteine residue (Fig. 1b), which is indistinguishable from the unmodified model preprotein in terms of import and arrest (Supplementary Fig. 1). On expression and purification of b2Δ-DHFRcys from Escherichia coli, a biotin label was attached to the C-terminal cysteine (Supplementary Fig. 2a,b). Saturating amounts of the preprotein b2Δ-DHFRbiotin were incubated with isolated yeast mitochondria in the presence of methotrexate (MTX). MTX stabilizes the folded state of DHFR, and therefore b2Δ-DHFRbiotin accumulates as an arrested preprotein in TOM–TIM23 import sites (Fig. 1c). The biotinylated moiety remains on the outer mitochondrial surface for subsequent labelling, and was fully accessible to streptavidin binding (Supplementary Fig. 2c). We then attached biocompatible streptavidin-QD525 to the biotin-tag on the accumulated preprotein to mark active import sites in mitochondrial samples (Supplementary Note 1). Excess QDs were removed by OptiPrep gradient centrifugation, whereby in the presence of accumulated preproteins, fluorescent QD525 labelling of mitochondria was observed (Fig. 1d). The labelled mitochondrial fractions and unlabelled control mitochondria were recovered from the gradients and analysed by cryo-ET.

As a comparison with STAMP, we also engineered both two and three tandem copies of the metallothionein protein to cytochrome b2Δ-DHFR (b2Δ-DHFR-MT2 and b2Δ-DHFR-MT3, respectively). b2Δ-DHFR-MT3 was found to express poorly as aggregates in all E. coli strains, at various temperature, induction conditions and metal (Au/Cd/Zn) conditions tested, thus was not pursued further. b2Δ-DHFR-MT2 could be expressed and purified at low amounts (60 μg l−1 cell culture compared with 4 mg l−1 for b2Δ-DHFRcys) in cells supplemented with Au+ (Supplementary Fig. 3a). On in vitro import of radiolabelled b2Δ-DHFR-MT2, the TOM–TIM23 supercomplex was formed in the presence of MTX (Supplementary Fig. 3b). In contrast to unmodified b2Δ-DHFR or b2Δ-DHFRcys, a substantial fraction of b2Δ-DHFR-MT2 also accumulated in TOM–TIM23 supercomplexes in the absence of MTX, and thus independently of the folding state of DHFR (Supplementary Fig. 3b). We conclude that the fused MT2 itself inhibits the full translocation of preprotein into the mitochondrial matrix. Incubation of the expressed and purified preprotein with isolated mitochondria resulted in efficient b2Δ-DHFR-$MT2(Au+)$ accumulation as a TOM–TIM23 supercomplex intermediate (Supplementary Fig. 3c).

### Visualization of active preprotein import sites in situ

In tomograms of b2Δ-DHFRbiotin-loaded mitochondria, the distribution of active import sites was visualized via the specifically attached QD525 with excellent spatial resolution (Fig. 2a,b, Supplementary Movie 1). Import sites were found around the entire circumference of mitochondria, yet their distribution appeared non-uniform and clustered. Close inspection of a large mitochondrial import site cluster revealed that it was located in the vicinity of, but not directly apposed to the CJ (Fig. 1c), which connects crista membranes to the inner boundary membrane19,20.

Analysis of tomograms of mitochondria loaded with b2Δ-DHFR-$MT2(Au+)$ did not enable the location of import sites to be determined. Mitochondria with arrested preprotein showed dark spots on the outer membrane (Supplementary Fig. 3e), which were comparable in size to the observed densities of b2Δ-DHFR-$MT2(Au+)$ alone in solution (~3 nm, Supplementary Fig. 3d). However, after analysis of control tomograms of non-importing mitochondria (Supplementary Fig. 3f), it was apparent that the metallothionein tag could not be distinguished from protein densities on the outer surface of mitochondria. Labelling using metallothionein was therefore not continued further.

### Determining the specificity of STAMP

The limitation of tomographic tilt angles (±~60°) results in a missing wedge of information21. This is centred around the z-axis in Fourier space, so that mitochondrial membranes that run parallel to the grid plane are poorly defined in the tomographic reconstruction. While QD525-labelled import sites were easily distinguished on membranes perpendicular to the grid plane, they were only visualized with confidence on all surfaces in a few cases (Table 1). This is due to the relatively small size of QD525 (4 nm spherical core). To resolve this problem, we used QD605 (4 × 9.4 nm ellipsoidal core), which is larger and more easily visible by EM (Supplementary Fig. 4b–d). Moreover, the brighter red fluorescence of QD605 is complementary to MitoTracker Green, which stains mitochondria regardless of membrane potential, allowing us to reliably determine the specificity of our labelling technique by fluorescence microscopy. In this way, statistical information on the specificity of QD605 labelling could be collected from a much larger sample size, thus counteracting the common issue of small sample sizes obtained by cryo-ET. After QD605 incubation and density gradient separation (Fig. 3a), the preprotein-loaded mitochondria were labelled, demonstrating that the mitochondria used in our assays were import competent (Fig. 3b, Supplementary Fig. 5). The distribution of QDs on mitochondria was uneven, in agreement with tomographic data. Seven percent of QD605 fluorescence did not co-localize with mitochondria, which may be due to the disruption of some organelles during sample preparation, giving rise to membrane fragments that still contain bound QD605-labelled preproteins. In contrast, 99.7% of control mitochondria that were not preloaded with preproteins did not show QD605 labelling under the same conditions, indicating an extremely low interference from nonspecifically associated QDs, also in agreement with tomographic data (Supplementary Movie 2).

### Calculation of the number of preprotein import sites

Cryo-ET analysis of mitochondria labelled with arrested preproteins and QD605 provided a clear three-dimensional view of active import sites in 12 different mitochondrial samples (Fig. 3c,d, Table 2, Supplementary Movie 3). For statistical analysis, we collected tomograms of ellipsoidal mitochondria to reveal a linear correlation between the mitochondrial surface area (equation (1), Methods) and the number of QDs, with an average value of 69 QD605-labelled active import sites per μm2 (Fig. 3e,f, Table 2). Calculations performed with the data set obtained with QD525 (Table 1) revealed an average value of 66 QD525-labelled active import sites per μm2, demonstrating that QD size was not limiting for the quantitative labelling of import sites.

Labelling with QD605 enabled us to investigate the non-uniform distribution of import sites in detail on a larger data set. The distance between QD neighbours was calculated for each mitochondrion (Supplementary Fig. 6), revealing a clear trend for import sites to be located ~30 nm apart (Fig. 3g). On average, 67% of import supercomplexes were seen in distinct clusters of two or more QDs located <50 nm apart (Fig. 3g, Table 2).

### Preprotein import occurs at specific microdomains

We occasionally observed mitochondrial fusion intermediates with two adjacent, but separate inner membranes surround by one single outer membrane22 (Fig. 4a, Supplementary Fig. 7 and Supplementary Movie 4). Interestingly, analysis of an importing mitochondrion with this feature revealed a marked increase (25% of the mitochondrion total) of QD605-labelled import sites in the vicinity of the two tightly aligned inner membranes, indicative of focused targeting to specific membrane regions.

Our results with QD525 furthermore indicated a spatial correlation between import sites and CJs (Fig. 2c). QD605 labelling of a mitochondrion with a long slit-like CJ on the upper surface revealed a large cluster of import sites located directly between the two branches of the junction (Fig. 4b). To investigate the spatial relationship between import sites and CJs in detail, we calculated the distances from QD605 to the closest CJ in nine different mitochondrial samples (Supplementary Fig. 8). This revealed that rather than directly neighbouring CJs, import sites tend to be located 30–60 nm away on average (Fig. 4c).

### Structural insights into the import supercomplex

Apart from showing the relative distribution of active preprotein import sites, our results also provide the first insights into their structural organization at the macromolecular level. Earlier studies of chemically fixed samples proposed a closer proximity of mitochondrial outer and inner membranes at translocation contact sites16,17. Our tomograms of unfixed and unstained mitochondria collected with a K2 direct electron detection camera now uncover unprecedented details of the two-membrane spanning translocation intermediates (Fig. 5, Supplementary Movie 5). First, there is no measurable difference in the distance between both lipid bilayers at active import sites compared with other regions away from the positions of QD labelling (Fig. 5, Table 3). Second, we observe distinct protein densities protruding from the outer membrane and spanning the intermembrane space (Fig. 5). To examine these more closely, all data collected under identical conditions were combined, which revealed that the outer membrane protein density located directly beneath the QD was well conserved in 91% of cases. The intermembrane space spanning protein density was in turn clearly determined in 96% of cases. These data were then subject to subtomogram averaging, which revealed the overall shape of conserved protein densities at the sites of QD labelling, that likely represent parts of the active protein import machinery (Supplementary Fig. 9).

## Discussion

The study of active biological machines in their native environment is a major new field of scientific discovery. To pinpoint proteins of interest, it is paramount to develop specific labels that are small enough not to compromise biological function, yet are large enough to be distinguished. Our new STAMP (Specifically TArgeted Membrane nanoParticle) approach constitutes an elegant means to specifically select for protein complexes in situ. In particular, STAMP is widely applicable for the localization of ligand-binding membrane protein complexes in their active states. It consists of three consecutive steps: (1) selective labelling of a ligand with a small non-toxic tag; (2) specific targeting of the labelled ligand to the corresponding membrane protein complex; (3) selective binding of the membrane-targeted ligand by dense QD nanoparticles.

We have used this approach in the study of mitochondrial preprotein import complexes, and compared it with the previously reported metallothionein tag2,5,6. The added density in b2Δ-DHFR-$MT2(Au+)$ was visualized in tomograms of the protein alone in solution, but on arrest across the TOM–TIM23 complex could not be distinguished with confidence from proteins bound to the mitochondrial outer membrane. This is due to the small size of the MT tag (~3 nm) compared with QD525 (~4 nm) or QD605 (~4 × 9.4 nm), in addition to the reduced electron scattering potential (compare Fig. 3c with Supplementary Fig. 3d). STAMP has the added benefit of being a multi-purpose tag, both dense and fluorescent, which we have used to full advantage in our studies.

Using STAMP, we were able to visualize mitochondrial preprotein import sites, which revealed their non-uniform distribution on the mitochondrial surface. Notably, these data are consonant with super-resolution microscopy analysis of whole cells, which detected clustering of the TOM complex receptor Tom20 in mitochondria23. Clusters of Tom20 were determined to be ~30–40 nm in diameter23, which is in the same range as data presented here (Figs 3c and 4a,b). It should, however, be noted that we report on the localization and organization of active preprotein-carrying TOM–TIM23 supercomplexes, which is not equivalent to the Tom20 receptor in isolation. Moreover, the TOM complex is much more abundant than the TIM23 complex and hands over incoming mitochondrial proteins to several distinct downstream import and sorting pathways. Thus, the mitochondrial TOM complex population likely contains different pools that cooperate with different partner protein complexes13,14,15. We speculate that the local accumulation of TOM–TIM23 import sites may enable focused preprotein targeting to areas within the mitochondria that are in the process of major morphological changes. This idea is in line with the observed accumulation of active import sites in the vicinity of crista membranes, which are highly dynamic structures that undergo extensive remodelling in response to metabolic and developmental cues20. Interestingly, CJ proteins, like Mic60 (mitofilin), have been reported to form inner–outer membrane contacts that contain a fraction of TOM complexes24. However, these contact sites are thought to be distinct from the translocation contact sites spanned by arrested preproteins17. An earlier immunolabelling study did not reveal any particular tendency for Tom20 or Tim23 to locate in the vicinity of CJs25. However, this would be difficult to conclude with confidence from two-dimensional projections of chemically fixed and stained sections. The spatial correlation between CJs and preprotein import sites may now encourage a reassessment of the functional relationship between different mitochondrial membrane contact sites.

We frequently observed protein densities, both protruding from the outer membrane and spanning the distance across the IMS, at active import sites. The dimensions of the IMS densities suggest more than a single extended translocating polypeptide chain. Suspended between the TOM and TIM23 complexes, the translocated polypeptide thus seems to interact with protective or physically reinforcing proteins to generate the specific molecular architecture of active import sites26,27. Notably, the observed outer membrane protein density measuring ~10 nm in height has similar dimensions as the cytosolic domain of the Tom70 import receptor as revealed by X-ray crystallography28. Further mutational studies and subtomogram averaging based on larger data sets will provide more comprehensive structural information on this dynamic molecular machinery at work.

We used a three-step protocol to specifically label the active machinery engaged in mitochondrial preprotein import. Other intracellular protein translocation systems can now be studied with little modifications to our protocol. Moreover, we assume that this approach will also prove particularly informative with respect to the investigation of antigen-binding B- or T-cell receptors on the cell surface, or ligands binding G-protein-coupled receptors. The STAMP method can also be adapted to label endogenously expressed proteins in a two-step approach, by inclusion of a short 15 amino acid biotin acceptor peptide29 available in a variety of bacterial and eukaryotic vectors (AviTag, Avidity, CO). The AviTag has already shown potential as a protein domain marker in single particle EM studies30.

Labelling proteins from within the cell remains a challenge, yet QDs that are able to penetrate membranes represent promising options for the future31. Using STAMP, purification of tagged cells, organelles or membranes is followed by incubation with dense nanoparticles. Our approach can therefore be adapted as a tool for site-specific labelling of a multitude of membrane or surface-exposed proteins.

## Methods

### Cloning of b2Δ-DHFR

Plasmid pb2Δ-DHFR was generated by cloning a fusion gene that encodes the first 167 residues of cytochrome b2 (with an internal deletion of residues 47–65), N-terminally fused to mouse DHFR, into the pGEM4 vector18,32 (Promega, Madison, USA). Plasmid pb2Δ-DHFRcys, encoding a mutated fusion protein that harbours the amino-acid substitutions C14S, C86S, C157S, and D337C was produced using the Quikchange site-directed mutagenesis kit (Agilent Technologies, Santa Clara, USA). Plasmids pb2Δ-DHFR-MT2 and pb2Δ-DHFR-MT3 were produced by assembly PCR, using the amplified gene products from plasmids pb2Δ-DHFR and pMAL-c2x-MT2, or pMAL-c2x-MT3, respectively2. For expression in E. coli, DNA fragments encoding b2Δ-DHFRcys, b2Δ-DHFR-MT2 and b2Δ-DHFR-MT3 were used to replace b2Δ-DHFR in the polylinker of the expression vector pUHE-7332, yielding plasmids pUHE-b2Δ-DHFRcys, pUHE-b2Δ-DHFR-MT2 and pUHE-b2Δ-DHFR-MT3, respectively. Primer sequences used in all cloning steps are given in Supplementary Table 1.

### Isolation and fractionation of mitochondria

The Saccharomyces cerevisiae strain YPH499 (MATa, ade2-101, his3-Δ200, leu2-Δ1, ura3-52, trp1-Δ63, lys2-801)33 was cultured in YPG medium (1% (w/v) yeast extract, 2% (w/v) Bacto-Peptone and 3% (w/v) glycerol) at 30 °C. At mid-logarithmic phase, cells were collected to isolate mitochondria by differential centrifugation34. In brief, on enzymatic digestion of the cell wall, cells were mechanically ruptured and cell debris was removed. Mitochondria were pelleted by centrifugation at 10,000g. The mitochondrial fraction was diluted in SEM buffer (250 mM sucrose, 1 mM EDTA, 10 mM MOPS-KOH, pH 7.2) to a protein concentration of 10 mg ml−1. Mitochondria aliquots were snap frozen in liquid nitrogen and stored at −80 °C.

### Expression of b2Δ-DHFR and biotinylation of b2Δ-DHFRcys

The fusion proteins b2Δ-DHFRcys and b2Δ-DHFR-MT2 were expressed from plasmids pUHE-b2Δ-DHFRcys or pUHE-b2Δ-DHFR-MT2 in E. coli BMH 71–18 cells (Clontech, CA, USA) by IPTG induction in LB media at mid exponential phase at 15 °C overnight. Cells expressing b2Δ-DHFR-MT2 were additionally supplemented with 0.5 mM sodium aurothiomalate. Cells were pelleted and washed in sucrose buffer (20 mM KPi, pH 8, 30% sucrose, 1 mM EDTA, 10 mM DTT, 1 mM phenylmethylsulphonyl fluoride (PMSF)) and resuspended in MOPS buffer (20 mM MOPS pH 8, 1 mM EDTA, 10 mM DTT, 1 mM PMSF, 0.1% Triton X-100). After cell disruption, insoluble material was removed by centrifugation at 22,000g for 30 min at 4 °C. The soluble protein was purified from the resulting supernatant via a SP-Sepharose high-performance cation exchanger (GE Healthcare, Buckinghamshire, UK) followed by size-exclusion chromatography over a HiLoad Supadex 75 (16/60) column (GE Healthcare). Purified b2Δ-DHFRcys was incubated with a 10-fold molar excess of maleimide-PEG2-biotin (Pierce, Rockford, USA) overnight at 4 °C, while gently rotating. Unbound label was removed by size-exclusion chromatography using a high-resolution Supadex 75 (10/300) column (GE Healthcare). Protein preparations were analysed by SDS–PAGE and Coomassie blue staining. Full scans of gels are shown in Supplementary Fig. 10. The degree of biotinylation was calculated to be 1.1±0.24 moles biotin/mole b2Δ-DHFRcys using the HABA biotin quantitation kit (Pierce).

### Labelling of arrested supercomplexes with QDs

QDs (Invitrogen, Carlsbad, USA) were centrifuged at 5,000g for 3 min before use and only the supernatant fraction was used. The starting material, supernatant and pellet were assessed by agarose gel electrophoresis (1% agarose in 40 mM Tris pH 8, 20 mM acetic acid, 1 mM EDTA) and EM (Supplementary Fig. 4). Mitochondria with and without arrested b2Δ-DHFRbiotin were incubated with a 10-fold excess of streptavidin-coated QDs over the molar amount of Tim23 in the mitochondrial sample, for 4 h at 4 °C with gentle shaking. This was calculated according to the experimentally determined amounts of Tim23 per mg of total mitochondrial protein35. The efficiency of streptavidin binding to the b2Δ-DHFRbiotin-carrying TOM–TIM23 supercomplex was assessed by incubation of preprotein-loaded intact mitochondria with the same relative amount of streptavidin as for the streptavidin-QD labelling reactions before blue native electrophoresis and western blotting. Separation of excess QDs was performed with an OptiPrep density gradient (Sigma-Aldrich, St. Louis, USA), using gradients with 10 steps of 200 μl each, ranging from 0 to 27% iodixanol in ultra-clear centrifuge tubes (Beckman Coulter, Pasadena, USA). Samples were centrifuged at 80,000g for 20 min at 4 °C with gentle braking. A quantity of 400 μl fractions corresponding to the mitochondrial bands were removed and diluted into 2 ml SEM buffer to dilute OptiPrep medium. Mitochondria were pelleted at 20,000g for 10 min  at 4 °C and resuspended in SEM buffer at a protein concentration of ~10 mg ml−1.

### Electron microscopy

QD525 or QD605 (20 nM) were applied to glow-discharged Cu 400 mesh (Plano, Wetzlar, Germany) continuous carbon-coated support grids and stained with 1% uranyl acetate. Electron micrographs were collected at a magnification of × 45,000 or × 56,000 (corresponding to a pixel size of 0.27 and 0.24 nm, respectively), at 8 μm defocus on a Tecnai Spirit microscope (FEI, Hillsboro, USA), operating at 120 keV and fitted with a 4k × 4k Ultrascan CCD camera (Gatan, Pleasanton, USA).

### Electron cryotomography

Mitochondria at 5 mg ml−1 total mitochondrial protein concentration were mixed 1:1 with 10 nm protein A-gold (Aurion, Wageningen, The Netherlands) as fiducial markers and applied to glow-discharged R2/2 Cu 300 mesh holey carbon-coated support grids (Quantifoil, Jena, Germany). The samples were blotted for ~4 s in a humidified atmosphere and plunge frozen into liquid ethane in a home-made plunge freezing device (Deryck Mills, MPI for Biophysics, Frankfurt). Grids were maintained under liquid nitrogen and transferred into the electron microscope at cryogenic temperatures.

Tomograms were collected at tilt steps of 1–2° and 8 μm defocus, using either a Tecnai Polara or Titan Krios microscope (FEI), both equipped with field emission guns operating at 300 keV and Quantum energy filters (Gatan, Pleasanton, USA) operated with slit widths of 20 eV. The Polara is fitted with a 4k × 4k Ultrascan CCD camera and the Krios with a K2 Summit direct electron detection camera (Gatan, Pleasanton, USA). The total dose per image was ~2e Å−2 for the CCD camera and 8e per pixel per second for the K2 camera.

Tomograms were aligned using the gold fiducial markers and volumes reconstructed by weighted back projection using the IMOD software36 (Boulder Laboratory, Boulder, USA). Contrast was enhanced by nonlinear anisotropic diffusion (NAD) filtering in IMOD37. Segmentation was performed using AMIRA (FEI).

The tomographic slice shown in Fig. 2a was collected on the Krios microscope at a magnification of × 26,000 (corresponding to a pixel size of 0.53 nm). Slices shown in Figs 4 were collected on the same microscope at a magnification of × 42,000 (corresponding to a pixel size of 0.33 nm). The tomographic slices shown in Fig. 3c and Supplementary Fig. 3d,f were collected on the Polara microscope at a magnification of × 34,000 (corresponding to a pixel size of 0.6 nm).

### Calculation of closest QD–QD and QD–CJ distances

Clusters were initially visually inspected and it was determined that they occur in groups of QDs 50 nm apart or less (centre-to-centre). A MATLAB (Mathworks, CA, USA) script was developed to accurately measure the distances between QDs and between QDs and CJs. The coordinates of QDs and CJs marked in IMOD were automatically loaded into MATLAB and the distances calculated in an iterative for-loop by Pythagoras theorem. The analysis was repeated for 12 data sets (12 mitochondria from 12 tomograms, accumulating 1,159 QD data points) to extract the minimal QD–QD distances. Of these 12, the 9 mitochondria with the clearest resolved cristae were used for the QD–CJ analysis (accumulating 836 QD data points). Averaged histograms were subsequently calculated, showing the mean frequency of occurrence for each minimal distance between QD–QD and QD–CJ. To account for the different numbers of QDs in each data set, the mean frequency was calculated as a percentage.

### Calculation of the surface area of a mitochondrion

In order to calculate mitochondrial surface area, 17 tomograms were collected of approximately ellipsoidal mitochondria of various sizes, which were labelled with QDs. The surface area of the ellipsoid was calculated from:

where a, b and c are the radii along the x, y and z axes of the ellipsoid, respectively (as depicted in Fig. 3e), and p is a constant 1.6075 (ref. 38).

### Subtomogram averaging

Conserved outer membrane and IMS protein densities are only clearly visible on membranes running parallel to the grid plane. The number of particles available for averaging per tomogram is therefore low. Thus, in order to calculate a preliminary model of the QD-marked supercomplex, data from multiple tomograms were combined. Subvolumes marked by 81 QDs from six twice-binned (pixel size=0.66 nm) and NAD contrast-enhanced tomograms, collected at a magnification of × 42,000 at 8 μm defocus with the K2 camera, were averaged using PEET39 (IMOD software, Boulder Laboratory). Subtomogram averages were calculated independently for the outer membrane and inner membrane space protein densities, then were subsequently combined into a composite model. Three-dimensional maps were analysed and displayed using Chimera (UCSF, San Francisco, USA). The resolution of the average is less than 4 nm, considering that the first zero of the contrast transfer function at the imaging conditions used is at ~3.9 nm, and that the tomograms were contrast enhanced by NAD.

### Confocal microscopy

Mitochondria at a concentration of 1 mg ml−1 total protein concentration were incubated with 50 nM MitoTracker Green (Invitrogen, Carlsbad, USA) for 15 min at 37 °C. A volume of 100 μl of sample was added to individual wells of 8-well chamber slides (Sarstedt, Nümbrecht, Germany) and 200 μl of 1–2% SeaKem low-melting point agarose (Lonza, Basel, Switzerland) dissolved in SEM buffer was added on top to prevent mitochondrial movement. Images were acquired at a magnification of × 63 using an inverted confocal microscope (LSM 710-plan apochromat /1.20 NA/ oil immersion; Carl Zeiss, Jena, Germany) and the ZEN2008 software (Carl Zeiss, Jena, Germany). The excitation laser wavelength was 488 nm and the emission windows were set at 492–535 nm for the MitoTracker Green and 597–700 nm for the QD605. The pinhole was set at 1 AU plus a master gain of 750.

How to cite this article: Gold, V. A. M. et al. Visualizing active membrane protein complexes by electron cryotomography. Nat. Commun. 5:4129 doi: 10.1038/ncomms5129 (2014).

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## Acknowledgements

We thank Drs Ulrike Endesfelder and Mike Heilemann (Institute of Physical and Theoretical Chemistry, University of Frankfurt) for help with confocal microscopy, Deryck Mills (MPI of Biophysics, Frankfurt) for maintenance of the EM facility, and Paolo Lastrico (Graphics Department, MPI of Biophysics, Frankfurt) for assistance with Supplementary Movies and Fig. 1a. We thank Drs Bertram Daum and Karen Davies for helpful discussions on tomography. The plasmids pMAL-c2x-MT2 and pMAL-c2x-MT3 were a gift from Dr Christina Risco (CNB-CSIC, Madrid). This work was supported by the Max Planck Society, Deutsche Forschungsgemeinschaft (Sonderforschungsbereich 746), Excellence Initiative of the German Federal & State Governments (EXC 294 BIOSS) and by an EMBO Long-Term Fellowship to V.A.M.G. (ALTF 1035-2010).

## Affiliations

1. ### Department of Structural Biology, Max Planck Institute of Biophysics, Max-von-Laue-Straße 3, 60438 Frankfurt am Main, Germany

• Vicki A.M. Gold
• , Andreas Walter
•  & Werner Kühlbrandt
2. ### Institute for Biochemistry and Molecular Biology and BIOSS Centre for Biological Signalling Studies, University of Freiburg, 79104 Freiburg, Germany

• Raffaele Ieva
• , Nikolaus Pfanner
•  & Martin van der Laan

## Authors

### Contributions

V.A.M.G., N.P., M.v.d.L. and W.K. designed the study. V.A.M.G. and R.I. performed the experiments and evaluated the data together with A.W., N.P., M.v.d.L. and W.K. V.A.M.G. and R.I. prepared the figures. V.A.M.G. and M.v.d.L wrote the manuscript. All authors commented on the manuscript.

### Competing interests

The authors declare no competing financial interests.

## Corresponding author

Correspondence to Vicki A.M. Gold.

## PDF files

1. 1.

### Supplementary Figures, Table, Note and References

Supplementary Figures 1-10, Supplementary Table 1, Supplementary Note 1 and Supplementary References

## Videos

1. 1.

### Supplementary Movie 1

Tomographic volume of a preprotein-importing mitochondrion labelled with QD525. The outer membrane (fuschia), the inner membrane (mauve), and the cristae membranes (aqua) are shown relative to the position of QDs (green spheres). The movie corresponds to Fig. 2b.

2. 2.

### Supplementary Movie 2

Tomographic volume of a non-importing control mitochondrion incubated with QD605.

3. 3.

### Supplementary Movie 3

Tomographic volume of a ellipsoidal preprotein-importing mitochondrion labelled with QD605. The outer membrane (blue) and the cristae membranes (yellow) are shown relative to the position of QDs (green spheres). The movie corresponds to Fig. 3d.

4. 4.

### Supplementary Movie 4

Tomographic volume of a preprotein-importing mitochondrion labelled with QD605. Segmentation of the septum region reveals the high concentration of QDs (green spheres). The outer membrane is rendered in two colours (light and dark blue) in order to distinguish the position of the septal ring with respect to the cristae (yellow). The movie corresponds to Fig. 4a.

5. 5.

### Supplementary Movie 5

Section of a sample tomographic volume depicting a close—up of the sites of preprotein import. The QD (green arrowhead) is shown in the vicinity of a protruding outer membrane density (blue arrowhead) and a density crossing the intermembrane space (red arrowhead). The movie corresponds to Fig. 5a.