Table 1: Description of three experimental designs used to investigate ovarian follicles in the 3D printed scaffolds.

From: A bioprosthetic ovary created using 3D printed microporous scaffolds restores ovarian function in sterilized mice

Table 1: Description of three experimental designs used to investigate ovarian follicles in the 3D printed scaffolds.
ExperimentFollicle sizeScaffold size, angleDuration (mice per group)
(a) In vitro: to test effects of architecture on survival to stimulate in vitro maturation3–4 150–180 μm follicles per scaffold to visualize health of each under light microscopy3–2 mm
30°, 60°, 90°
2–8 days
(b) In vivo: to test vascularization and hormone restorationDay 1: primordial, primary and small secondary follicles;
Day 2: filled in with more follicles ≤180 μm;
40–50 follicles total
2 mm (to fit in bursa)
60°
1 or 3 weeks post-surgery
(16 OVX+implant; 5 OVX)
(c) In vivo: to test restorative organ function—mating, ovulation, live birth, lactationDay 1: primordial, primary and small secondary follicles;
Day 2: filled in with more follicles ≤180 μm;
40–50 follicles total
2 mm (to fit in bursa)
60°
8–10 weeks post-surgery
(7 OVX+implant; 2 OVX)
  1. Ovariectomized animals with ovarian bioprosthesis implant are labelled as ‘OVX+implant’. Ovariectomized animals with a sham implant (3DP scaffold without cells) are labelled as ‘OVX’.