Figure 3 : Follicles interact with struts.

From: A bioprosthetic ovary created using 3D printed microporous scaffolds restores ovarian function in sterilized mice

Figure 3

(a) Light microscopy image of follicle cultured within 30° scaffold for 8 days with maintained basement membrane (green arrows) and centralized oocyte (white arrow). (b) Maximum intensity projection of confocal fluorescence image stack of follicle stained for actin (red) and DNA (blue) cultured within 60° scaffold for 6 days showing that cells surround and support follicle. (c) Maximum intensity projection of confocal fluorescence image stack with light image overlay. Immunostaining of vinculin (green), a cytoskeletal protein associated with cell–matrix adhesions, in the cells that adhere to the gelatin struts of the 3D printed scaffold after 8 days of culture. DNA (blue). (d) Maximum intensity projection of confocal fluorescence image stack of follicle cultured for 2 days in 60° scaffold. Immunostaining for HMGB1 (green), counterstained for actin (red) and DNA (blue), indicates that cells surrounding and anchoring the follicle are stromal cells. §, scaffold strut. Scale bars: (a) 50 μm; (bd) 100 μm.