Figure 2 : Ovarian follicle survival is dependent on multiple follicle–strut contacts provided by 30° and 60° scaffold pore geometries.

From: A bioprosthetic ovary created using 3D printed microporous scaffolds restores ovarian function in sterilized mice

Figure 2

(ac) 3D reconstructions of confocal fluorescence image stacks of 30° (a,d,g), 60° (b,e,h) and 90° (c,f,i) advancing angle scaffolds. Struts 250 μm and distance between struts (from edge to edge) 350 μm. (df) 3D reconstructions of confocal fluorescence image stacks of corresponding pores. Colour corresponds to depth of pore according to heat map and total thickness of image is 200 μm. (gi) Maximum intensity projections of confocal fluorescence image stacks of GFP+ follicles seeded in pores, after 2 days in culture. Follicles in 30° and 60° pores tended to reside in corners whereas follicles in 90° pores were more likely to be along only one strut (j), survival as a function of scaffold geometry. Follicles thrived best in 30° and 60° scaffolds. P=0.0146. (k) Percentage of follicles making 1, 2 or 3 scaffold contacts per geometry. When seeded into 30° and 60° scaffolds, follicles were most likely to have 2 or 3 scaffold contacts whereas follicles in 90° scaffolds had an equal chance of making only 1 contact versus 2 or more. (l) Follicle survival significantly increased with the number of scaffold contacts. P=0.0053. (m) As the number of strut contacts increased, the length of follicle adhesion along one strut decreased. P=0.0029. Scale bars: (gi) 100 μm. All data are presented as average±s.e.m. Statistical significance performed using one-way ANOVA with a Holm–Sidak’s multiple comparisons test (*P<0.05; **P<0.005).