Article | Open

Mosaic evolution in an asymmetrically feathered troodontid dinosaur with transitional features

  • Nature Communications 8, Article number: 14972 (2017)
  • doi:10.1038/ncomms14972
  • Download Citation
Received:
Accepted:
Published online:

Abstract

Asymmetrical feathers have been associated with flight capability but are also found in species that do not fly, and their appearance was a major event in feather evolution. Among non-avialan theropods, they are only known in microraptorine dromaeosaurids. Here we report a new troodontid, Jianianhualong tengi gen. et sp. nov., from the Lower Cretaceous Jehol Group of China, that has anatomical features that are transitional between long-armed basal troodontids and derived short-armed ones, shedding new light on troodontid character evolution. It indicates that troodontid feathering is similar to Archaeopteryx in having large arm and leg feathers as well as frond-like tail feathering, confirming that these feathering characteristics were widely present among basal paravians. Most significantly, the taxon has the earliest known asymmetrical troodontid feathers, suggesting that feather asymmetry was ancestral to Paraves. This taxon also displays a mosaic distribution of characters like Sinusonasus, another troodontid with transitional anatomical features.

Introduction

The Middle-Upper Jurassic and Lower Cretaceous of western Liaoning and neighbouring areas have produced spectacular fossil remains of theropod dinosaurs, particularly of maniraptoran theropods, which are critical to our understanding of bird origins1,2. Among them are many fossils of troodontids, which are considered as the closest relatives of birds either on their own3 or together with dromaeosaurids4,5,6,7,8. Reported troodontid species include Sinovenator changii9, Mei long10 and Sinusonasus magnodens11 from the early Aptian Yixian Formation (the middle section of the Jehol Group)12, Jinfengopteryx elegans13 from the Hauterivian Dabeigou/Dadianzi Formation (the low section of the Jehol Group)14, and Anchiornis huxleyi7,15, Xiaotingia zhengi6 and Eosinopteryx brevipenna16 from the Oxfordian Tiaojishan Formation. Significantly, the discoveries of the latter four taxa provide direct evidence for the presence of feathers in troodontids, which shed new light on the early evolution of pennaceous feathers6,7,13,16. However, several recent phylogenetic studies have questioned the troodontid affinities of these species3,6,17,18, and thus feathers have yet to be found in an unquestionable troodontid taxon.

Here we describe a new troodontid from the Yixian Formation of Baicai Gou, Yixian County, western Liaoning preserving large feathers along the forelimbs, hindlimbs and tail that have a distribution pattern similar to other basal paravians such as Microraptor, Anchiornis and Archaeopteryx1,18,19,20. Its discovery is highly significant in reconstructing both the skeletal and integumentary evolution of troodontids, and the more inclusive paravians.

Results

Systematic palaeontology.

       Theropoda Marsh, 188121

      Coelurosauria Huene, 192022

      Maniraptora Gauthier, 198623

      Troodontidae Gilmore, 192424

    Jianianhualong tengi gen. et sp. nov.

Etymology. ‘Jianianhua’ (), the Chinese company that supported this study; ‘long’ (), the Chinese Pinyin for dragon. The specific name honors Ms Fangfang Teng, who secured the specimen for study.

Holotype. DLXH 1218, a nearly complete skeleton with associated feathers (Fig. 1) housed at the Dalian Xinghai Museum.

Figure 1: Jianianhualong tengi holotype DLXH 1218.
Figure 1

(a) Photograph and (b) line drawing of the specimen. Scale bar, 50 cm. cav, caudal vertebrae; cv, cervical vertebrae; dv, dorsal vertebrae; fu, furcula; lfe, left femur; lh, left humerus; li, left ilium; lis, left ischium; lm, left manus; lp left pes; lpu, left pubis; lr, left radius; lu, left ulna; md, mandible; rc, right coracoid; rfe, right femur; rfi, right fibula; rh, right humerus; ri, right ilium; rm, right manus; rr, right radius; rs, right scapula; rt, right tibiotarsus; ru, right ulna; sk, skull; ss, synsacrum.

Locality and horizon. Baicai Gou (), Yixian County, western Liaoning, China; Lower Cretaceous Yixian Formation12.

Diagnosis. A troodontid distinguishable from other taxa in possessing the following apomorphic features (*indicates autapomorphic feature): maxillary rostral ramus triangular in outline and relatively high dorsoventrally*; maxillary ascending process extending posterodorsally at a high angle (an angle of 45° to maxillary ventral margin)*; lacrimal with a long descending process sub-equal in length to anterior process; a prominent ridge along anterior edge of the lateral surface of the lacrimal descending process; a distinct fossa on the dorsal surface of the surangular close to its posterior end; axial neural spine with a convex dorsal margin, transversely thickened anterior margin, and posterodorsal portion expanding strongly posteriorly; long manual phalanx II-1 (slightly shorter than metacarpal III) with prominent proximoventral heel, large groove along the medial surface of more than proximal half of manual phalanx II-1*; highly elongated manual III-2 (slightly longer than metacarpal III)*; robust ungual phalanges (medial ungual proximal depth/ungual length ratio >0.5); ilium with slightly concave dorsal margin in lateral view*; small medial lamina along ischial obturator process dorsal margin; metatarsal IV without prominent ventral flange*.

Description

DLXH 1218 is inferred to be an adult individual based on fusion features (for example, the centra and neural arches are completely fused to each other in all visible vertebrae)25,26. DLXH 1218 measures 100 cm in preserved skeletal body length, and is estimated to be 112 cm in total skeletal body length with a fully reconstructed tail. With a femoral length of 117 mm, its body mass is estimated to be 2.4 kg based on an empirical equation27. The estimated total skeletal body length and mass indicate that DLXH 1218 is similar in size to most other Jehol troodontids9,10,11,13. A succinct osteological description is given here (see Supplementary Table 1 for select measurements); for additional details, see Supplementary Note 1.

Cranium

The skull and mandible are in general well preserved (Fig. 2), but both premaxillae are missing and a few elements are preserved upside down, for example, the frontals. Otherwise the cranium indicates the presence of a sub-triangular cranial lateral profile, as in many other basal paravians. As in Mei long10, it has a relatively short snout and highly expanded skull roof. The cranium is relatively small (the mandible is about 75% of femoral length), as in many other basal paravians.

Figure 2: Cranium of Jianianhualong tengi holotype DLXH 1218.
Figure 2

(a) Photograph and (b) line drawing of the skull and mandible. Scale bar, 2 cm. ax, axis; fg, groove with foramina; fo, fossa; imf, internal mandibular fenestra; la, left angular; ld, left dentary; lect, left ectopterygoid; lex, left exoccipital; lf; left frontal; lhy, left hyoid bone; lj, left jugal; lla, left lacrimal; lm, left maxilla; lmf, left maxillary fenestra; ln, left nasal; lp, left parietal; lpt?, left pterygoid?; lpa, left prearticular; lpo, left postorbital; lpsaf, left posterior surangular foramen; lsa, left surangular; lsp?, left splenial?; lso, left supraoccipital; lsq, left squamosal; mc, Meckelian canal; ra, right angular; rart, right articular; rd, right dentary; rf, right frontal; rhy, right hyoid bone; rm?, right maxilla?; rn; right nasal; rp, right parietal; rpa, right prearticular; rq?, right quadrate?; rsa; right surangular; rso, right supraoccipital; rsp; right splenial; rsq, right squamosal.

The left maxilla is exposed, displaying a triangular outline. The rostral ramus, the anterior portion of the maxilla28, is triangular in outline. It is slightly longer anteroposteriorly than dorsoventrally, unlike the rostral rami in other troodontids, which are considerably longer anteroposteriorly than dorsoventrally29,30. The ascending process extends posteriorly considerably beyond the anterior border of the antorbital fenestra. It extends posterodorsally at an angle of 45° to the ventral margin of the maxilla, an angle considerably greater than in other troodontids (for example, 36° in Sinovenator9; 6° in Saurornithoides31). Similar to Byronosaurus32, a deep and narrow groove is present on the lateral surface of the jugal (ventral) ramus near the posterior end. There is a posteriorly deep jugal ramus as in derived troodontids29.

The antorbital fossa is extensive, and sub-triangular in outline with a dorsally displaced anterior margin as in derived troodontids29. As in relatively derived troodontids29, two large openings—the maxillary and antorbital fenestrae—are visible within the antorbital fossa in lateral view. In basal troodontids such as Sinornithoides, Sinovenator and Sinusonasus one additional opening—the promaxillary fenestra—is visible laterally9,11,33 (as in the basal paravians Anchiornis and Jinfengopteryx7,13). The maxillary fenestra is enlarged as in other troodontids29,34 (particularly derived ones29,31,34), is significantly elongated anteroposteriorly, and is displaced anteroventrally (its anterior and ventral borders are confluent rather than distant from those of the antorbital fossa, unlike basal troodontids such as Sinovenator).

Both lacrimals are exposed. The lacrimal is roughly a T-shaped bone, with an angle of 80° between the anterior and descending processes, and an angle of 105° between the posterior and descending processes. The long anterior process extends anteriorly beyond the anterior border of the antorbital fenestra, a feature that characterizes Troodontidae. At the junction of the descending and posterior process is a prominent lateral flange, as in all troodontids that preserve this anatomical region35. A similar structure is also present in most dromaeosaurids35. The long descending process is sub-equal in length to the anterior process, which is proportionally longer than in other troodontids29. It curves anteriorly in lateral view as in many deinonychosaurs. A prominent ridge extends along the anterior edge of the lateral surface of the descending process, which is anteroposteriorly wider than transversely thick.

Both nasals are preserved but neither provides much morphological information. The nasal is short anteroposteriorly and relatively broad transversely as in Mei long10. Similar to other troodontids29, there is a row of foramina along the lateral edge of this bone.

The prefrontals appear to be absent, but the possibility that small ones are present cannot be excluded.

Both frontals are nearly complete, and exposed in ventral view. The frontal is about 1.8 times as long anteroposteriorly as transversely wide. As in other troodontids29, the postorbital process has a smooth transit from the orbital edge of the frontal.

The parietals are not well exposed, but were clearly fused to each other into one unit. They have transversely convex lateral surfaces, and have a weak sagittal crest.

The ventral margin of the anterior process of the jugal is thickened transversely due to a prominent ridge along the ventral border of the lateral surface of the jugal; the sub-orbital ramus is still deeper dorsoventrally than wide transversely. A similar ridge is also present in other troodontids36. As in Sinovenator and other troodontids30, the posterior process is short and deep, and has a lateral surface that is depressed into the articular facet for the quadratojugal.

The left quadrate is a posteriorly curved bone with a height of 22 mm. It is preserved upside down. The dorsal end of the quadrate is single-headed. The subtriangular pterygoid ramus is relatively small, narrow dorsally and wide ventrally, with the peak close to the ventral end of the quadrate. In most non-avialan theropods, the peak is more dorsally positioned.

A tri-radiate bone is identified as the left postorbital. The jugal process is the longest one of the three processes but does not seem as long as in other basal troodontids such as Sinovenator30.

The mandible is subtriangular in lateral view, with a nearly straight dorsal margin and a convex ventral margin in lateral view. Both dentaries are preserved, and the left one is completely exposed. The dentary is a long and shallow bone, roughly triangular in lateral view as in other troodontids29. As in other troodontids29, there is an anteriorly narrow and posteriorly wide groove on the lateral surface of the dentary.

The left surangular exposes its lateral side and the right one its medial side. The lateral surface of the surangular is dorsoventrally convex. The surangular is deep dorsoventrally, forming most of the lateral surface of the posterior portion of the mandible. There is a distinct fossa on the dorsal surface of the surangular close to its posterior end.

The lateral surface of the left angular is exposed, and the medial surface of the right one. The angular is a long and curved bone in lateral view. A robust, strongly dorsally curved anterior process of the angular excludes the dentary from the external mandibular fenestra as in Sinovenator (for example, Institute of Vertebrate Paleontology and Paleoanthropology, IVPP V20378), some other troodontids, and to a lesser degree in some dromaeosaurids30; this probably represents a diagnostic feature for the Troodontidae or even the Deinonychosauria.

The medially exposed right splenial and the laterally exposed left splenial show that this bone is a sub-triangular plate, with its dorsal extremity near the dorsal margin of the mandible.

The medial side of the right prearticular suggests this is a typical ventrally curved maniraptoran one with a thin-bladed anterior portion and a somewhat rounded shaft-like posterior region. The dorsal margin of this bone is more strongly curved than the ventral margin.

The vertical columnar process of the right articular has a trapezoidal outline in medial view. Other morphological details are difficult to determine owing to their poor preservation, including those of the suspected left articular.

The teeth preserved show an unevenly distributed (anterior teeth are closely packed, whereas the middle and posterior ones are more widely spaced), heterodont dentition (anterior teeth slender and leaf-like, whereas the middle and posterior teeth have stout, posteriorly curved crowns). Twelve teeth are preserved in the left maxilla and nine more are estimated to have been present; nineteen teeth are preserved in the left dentary and six more might have been present. Consequently, there are probably 21 maxillary teeth and 25 dentary teeth on each side of the jaw. The preserved maxillary teeth are strongly curved posteriorly and have relatively short and basally constricted crowns as in other troodontids. The dentary teeth are generally similar in morphology to the maxillary teeth: they have relatively short tooth crowns, lack serrations in anterior teeth, and have relatively fine posterior serrations in middle and posterior teeth.

Postcranium

The vertebral column is nearly completely represented, but individual vertebrae are not well preserved (Fig. 3; Supplementary Note 1). Vertebral regions are roughly identifiable, but vertebral count is unknown. The cervicals are 16 cm long, the dorsal region is 17 cm long and the tail is 54 cm long.

Figure 3: Vertebral column of Jianianhualong tengi holotype DLXH 1218.
Figure 3

(a) Presacral vertebrae. (b) Caudal vertebrae. Scale bar, 2 cm. ax, axis; cd, caudal vertebra; ch, chevron; cr, cervical rib; cv, cervical vertebra.

The axis has a unique neural spine with a convex dorsal margin in lateral view, an anterior portion that is transversely thickened and a posterodorsal portion that expands strongly posteriorly. The fifth to seventh cervicals are the longest ones of the cervical series. The latter two cervicals have complex pneumatic systems (foramina and fossae separated by ridges) on their anterolateral surfaces. Most cervical ribs, except the ones attached to the third and sixth cervicals, are at least partially fused to their corresponding vertebrae.

The dorsal series comprises anteroposteriorly short anterior dorsal vertebrae and long middle and posterior ones. The anterior dorsals lack distinct fossae or foramina on their central lateral surfaces, and probably so do the middle and posterior dorsals. Nine pairs of dorsal ribs are preserved. As in other troodontids29, there are neither uncinate processes nor an ossified sternum. There are about 12 pairs of imbricated gastral segments preserved.

The sacral vertebrae are exposed due to the ventrally displaced left ilium. However, little morphological information is available due to the smashed nature of these bones.

An articulated series of 23 caudals is relatively well preserved, but the anterior caudals (5 in number) are badly smashed and the distal caudals (3 in number) are missing. Consequently, DLXH 1218 possibly has 31 caudals in total, making an estimated tail length 3.9 times of femoral length. The tail possesses several typical troodontid traits including posterior chevrons with relatively blunt-ended anterior processes and slight bifurcated posterior processes (see Supplementary Note 1 for more details).

The shoulder girdle displays several features more similar to those of derived troodontids29 (Fig. 4a). Both scapulae are preserved, with the left one incomplete and the right one partially exposed. The scapula is sub-equal in length to the humerus, and similar in thickness to the latter. Each scapula is preserved with its long axis angled at 40° to the dorsal series as in most articulated specimens of non-avialan theropods. However, in articulated specimens of long-armed paravians, the scapula is nearly parallel to the dorsal series30. The acromion process continues smoothly from the scapular blade, extending anteriorly probably beyond the anterior limit of the glenoid fossa. Below the acromion process, the scapula is strongly depressed in lateral view. The scapular blade is strap-like, with an expanded distal end measuring 130% of the minimum blade width.

Figure 4: Non-vertebral postcranial skeleton of Jianianhualong tengi holotype DLXH 1218.
Figure 4

(a) Shoulder girdle, (b) right manus, (c) pelvis and (d) left pes. Scale bar, 5 cm except in d, where it is 1 cm instead. ac, acromial process; acd, anterior caudal vertebrae; co, coracoid; dc 4, distal carpal 4; dr, dorsal ribs; dt 3, distal tarsal 3; dt 4, distal tarsal 4; dv, dorsal vertebrae; fu, furcula; ga; gastralia; lfe, left femur; lh, left humerus; li, left ilium; lis, left ischium; lpu, left pubis; ls, left scapula; mc II to IV, metacarpals II to IV; mp II-1 to II-2, manual phalanges II-1 to II-2; mp III-1 to III-3, manual phalanges III-1 to III-3; mp IV-1 to IV-4, manual phalanges IV-1 to IV-4; mt I to V, metatarsals I to V; pp I-1 to I-2, pedal phalanges I-1 to I-2; pdis, dorsal posterior process of ischium; pvis; ventral posterior process of ischium; r, radius; ra?, radialae?; rfe, right femur; rh, right humerus; ri, right ilium; ris, right ischium; rpu, right pubis; rs, right scapula; slc, semi-lunate carpal; u, ulna.

The left coracoid is mostly missing and the nearly complete right is exposed in medial view. As in other derived maniraptorans23, the coracoid is large and quadrangular, with the ratio of anteroposterior length to dorsoventral depth at the level of the scapular suture 1.3 (ref. 37), similar to those of the other pennaraptorans. The hooked postglenoid process of the coracoid is proportionally similar in size to that in Sinornithoides, but is longer than in Sinovenator30.

The furcula is poorly preserved, but is robust, flattened in cross-section and U-shaped, as in Mei38. This contrasts with the more delicate furcula of Sinornithoides38.

The left humerus is somewhat smashed, and mostly the anterior side of the right humerus is exposed. The humerus is 70% of femoral length, and as in derived troodontids it is considerably more slender than the femur. The proximal portion of the humerus curves considerably posteriorly and medially. The inner tuberosity is long proximodistally and the deltopectoral crest is subtriangular in outline and short. It is 20% of the total humeral length as in basal deinonychosaurs, but unlike the proportionally much longer ones seen in derived troodontids such as Linhevenator36.

Both ulnae are exposed and relatively well preserved. The ulna measures 88% of humeral length. It is slightly bowed posteriorly, whereas in Mei it is more strongly bowed and in most other troodontids it is straight10,23.

Both radii are relatively well preserved. The radius is slightly thinner than the ulna (76% of the shaft diameter). Unlike the straight radii in most other theropods, the proximal portion of the radius in Jianianhualong curves posteriorly.

The manus is typical of maniraptoran theropods, comprising three digits with a phalangeal formula of 0-2-3-4-0 (Fig. 4b). We follow the numbering for the wing digits of living theropods, as in most ornithological literature and some recent palaeontological studies39,40. The manus measures 112 mm in length, 140% of humeral length and 95% of femoral length.

The right wrist preserves three carpals, representing the semilunate carpal, distal carpal 4 and possibly radiale.

Metacarpal II is the most robust of the three metacarpals, and is 45% of the length of metacarpal III. There is a distinctive ventral flange along the lateral edge of the proximal half of the ventral surface of metacarpal II, partially contributing to a large flat facet for contacting metacarpal III.

Metacarpal IV is a long element and it is much more slender than metacarpal III. It appresses closely to metacarpal III for its whole length.

Manual phalanx II-1 is a long and robust element. It is slightly shorter than metacarpal III. It bears a prominent proximoventral heel so that the proximal end is 2.3 times as deep dorsoventrally as the shaft immediately proximal to the distal end, which represents the thinnest portion of the bone. In lateral view, manual phalanx II-1 curves slightly ventrally. There is a prominent, wide groove along the medial surface of the proximal half of this phalanx.

Manual phalanges III-1 and III-2 are similar in general morphology to II-1, but lack the distinctive proximoventral heel and are straight in lateral view. The former further differs from II-1 in having shallower and more ventrally positioned collateral ligament pits. The highly elongated manual III-2 is slightly longer than metacarpal III, a feature only known in certain ornithomimosaurs, and in several basal paravians such as Xiaotingia and Yixianosaurus37.

Manual phalanx IV-1 is sub-equal in length to IV-2, but combined they are shorter than IV-3 as in most derived maniraptorans and ornithomimosaurs.

The three ungual phalanges are robust (ungual 2 is the most robust and has a proximal depth more than half its length), highly curved, laterally compressed, and with prominent flexor tubercles.

The pelvis is in general similar to those of basal troodontids such as Sinovenator9. It has a proportionally small ilium, a posteroventrally oriented pubis, and a short ischium with a distally positioned obturator process (Fig. 4c).

The right ilium is complete, but obscured by sacral vertebrae and the proximal caudals; the left ilium is missing most parts. The ilium is small in size in comparison to the femur (ilium/femur length ratio is 0.60), which is the condition in Archaeopteryx and basal dromaeosaurids41. In most other theropods, the ilium is more than 70% the length of the femur. The dorsal margin of the ilium is slightly concave in lateral view, unlike the straight or convex ones seen in many other theropods. The pubic peduncle is anteroposteriorly wide and deep, and is slightly posteriorly directed, with a large depression on its anterolateral surface representing the anteriorly and laterally positioned fossa for the M. cuppedicus muscle42.

The left and right pubes are, as in other troodontids, appressed to form a broad, flat pubic apron. The dorsal edges along the midline meet and extend posteriorly to form a longitudinal ridge. The transversely hypertrophied pubic apron is characteristic of troodontids among theropods43, and the midline ridge is at least also known in Sinovenator.

Both ischia are preserved. The ischium is a short plate, 48% of pubic length. Proximally, close to the iliac articulation, is a small dorsal process as in basal dromaeosaurids and some basal birds41,44,45. Distal to this process, the dorsal margin is slightly concave in lateral view, and then expands posteriorly to form a second dorsal process close to the distal end (Fig. 4c), as in Archaeopteryx20. A distally positioned obturator process is present as in basal deinonychosaurs and some basal birds, but it is relatively small, closer in size to that of Archaeopteryx20. There is a small lamina along the dorsal margin of the obturator process, a feature also known in some dromaeosaurids such as Sinornithosaurus millenii and Buitreraptor, although in these taxa this lamina is much larger.

Both femora are preserved, but neither preserves many morphological details (Fig. 4c). The femur is significantly bowed anteriorly as in most theropods46. The distal half of the femur is considerably more robust than the proximal half, a feature also known in Anchiornis huxleyi15.

Both tibiotarsi are badly smashed and little morphological information is obtainable, which is also the case with the fibulae. Some badly smashed bones might be proximal tarsals.

Two disk-like distal tarsals are identified as distal tarsals 3 and 4 because they cover the proximal ends of metatarsals III and IV, respectively (Fig. 4d). The possibility that these two elements are partially fused to each other cannot be excluded.

Metatarsal I is a short, proximally tapered bone that attaches to the medial surface of metatarsal II about three-fifths of the way down the metatarsus (Fig. 4d). Metatarsal II is the most slender element of the three middle metatarsals and is also considerably shorter than the other two (metatarsal II/III length ratio 0.88). The distal end of metatarsal II is not ginglymoid as in other troodontids46. Metatarsal IV is the most robust element as in other troodontids46, but a prominent ventral flange appears to be absent. The slender and rod-like metatarsal V is 40% of the length of metatarsal III.

Pedal digit I is proportionally long (phalanges I-1 and I-2 combined/metatarsal III length ratio 0.31; Fig. 4d), which is almost the same as in Troodon (CMN 8539). Distally it extends beyond the distal end of metatarsal III. Pedal phalanx I-1 is proportionally long (phalanx I-1/metatarsal I length ratio 1.9). The ungual is robust (proximal end depth/ungual length ratio 0.50) and moderately curved.

Pedal digit II is typical of a derived troodontid. Phalanx II-1 is 150% of the length of phalanx II-2, with a dorsoventrally deep proximal end. Pedal phalanx II-2 has a proximoventral heel, which has both considerable proximal and ventral extensions as in derived deinonychosaurs47,48,49,50. As in other troodontids and basal dromaeosaurids51, the distal end extends moderately below and above the shaft indicating a wider rotation arc than in other theropods. The ungual of digit II is similar to those of some derived troodontids and dromaeosaurids36,49 in being strongly curved, with a prominent flexor tubercle; it is also much larger than pedal phalanges II-1 and II-2 (for example, II-3/II-1 length ratio 1.4). In basal troodontids such as Sinovenator, the ungual of digit II is about the length of pedal phalanx II-1.

Plumage

Feathers are preserved along nearly the whole vertebral series, forelimb and hindlimb (Fig. 5). Although most of these feathers are large, few morphological details are preserved other than the ones associated with the caudal series. Laser-stimulated fluorescence (LSF) imaging did not reveal any hidden feather details (Fig. 4 of ref. 52), but made existing details easier to see.

Figure 5: Plumage of Jianianhualong tengi holotype DLXH 1218.
Figure 5

(a) Feathers over dorsals, (b) feathers attached to anterior caudals, (c) asymmetrical tail feather, (d) line drawing of asymmetrical tail feather, (e) tail frond and (f) negative LSF image of tail frond. All scale bars, 2 cm.

Feathers ventral to the cervical series are at least 30 mm long. The ones dorsal to the dorsal and sacral vertebrae seem to be much longer than the cervical feathers, and the ones above the posterior dorsals and sacrals are 75 mm long. Large pennaceous feathers are preserved both dorsal and ventral to nearly the whole preserved caudal series as in Jinfengopteryx, Archaeopteryx and Anchiornis, forming a frond-like feathery tail, a primitive morphotype of tail feathering. These feathers are curved such that their distal edge is convex and their proximal edge is concave, unlike Archaeopteryx and possibly Jinfengopteryx too18,20,53. Most caudal feathers are difficult to measure, but the ones associated with the middle caudal vertebrae are 120 mm long and at least 10 mm wide. Interestingly, one lateral caudal feather has relatively strong asymmetry, with the trailing vane about twice as wide as the leading vane with barb angles of 10° and 15°, respectively (Fig. 5c,d). This feather and some other lateral caudal feathers appear to be distally blunt, with the distal portions even wider than the more proximal regions. The lateral caudal feathers of Archaeopteryx have an asymmetrical outline and rachis position, but its distal caudal feathers have a symmetrical, rounded outline at the tip as well as a seemingly symmetrical rachis position18.

Feathers are also preserved near the forelimb (humerus and ulna), but little morphological information is obtainable. Posterior to the preserved distal half of the tibia, there are also some poorly preserved feathers. Those near the middle portion of the tibia appear to be 70 mm in length, but few details are preserved.

The newly reported asymmetry in the tail feathers of Jianianhualong was incorporated into a parsimony-based ancestral state reconstruction of paravian arm and tail feather symmetry (see Supplementary Fig. 1; Supplementary Table 2 and Methods). This analysis revealed vane asymmetry as the ancestral condition for paravian arm feathers and asymmetrical tail feathers as the ancestral condition of a more inclusive paravian clade that excludes Scansoriopterygidae and Avialae. However, if the ancestral condition of scansoriopterygid and dromaeosaurid arm and tail feather symmetry is entered as being equivocal, then the reconstructed ancestral condition for paravian arm feather symmetry is also equivocal whereas it is asymmetrical for paravian tail feathers.

Discussion

Jianianhualong shares with other troodontids many apomorphic features29, which are probably troodontid synapomorphies. These features include a long anterior process of the lacrimal that extends anteriorly beyond the anterior border of the antorbital fenestra, a prominent lateral flange over the descending process of the lacrimal, a row of foramina along the lateral edge of the nasal, a postorbital process of the frontal that transitions smoothly from the orbital rim, a ridge close to the ventral edge of the jugal lateral surface, a triangular dentary in lateral view, an anteriorly narrow and posteriorly wide groove on the lateral surface of the dentary, a strongly curved, robust anterior process of the angular that contacts the surangular to exclude the dentary from the external mandibular fenestra, a relatively large number of teeth (25+ dentary teeth), uneven dentition distribution, heterodont dentition, dorsoventrally flattened chevrons with blunt-ended anterior processes and shallowly bifurcated posterior ones, and a pubic apron that is transversely broad and flat. Our numerical phylogenetic analysis places Jianianhualong in an intermediate position together with several species between the basalmost and derived troodontids (Fig. 6, see Methods).

Figure 6: Strict consensus of 3,066 most parsimonious trees produced by phylogenetic analysis.
Figure 6

The strict consensus tree (tree length=1,426, CI=0.279) shows the systematic position of Jianianhualong tengi among the Coelurosauria.

The discovery of Jianianhualong provides direct evidence for the presence of pennaceous feathers in an unquestionable troodontid theropod. Previous studies indicate that Anchiornis huxleyi, Eosinopteryx brevipenna, Jinfengopteryx elegans and Xiaotingia zhengi have pennaceous feathers6,7,13,16. However, some recent studies have questioned the troodontid affinities of these species3,6,17,18. Jianianhualong is clearly a troodontid theropod as indicated by numerous troodontid features, and its discovery thus confirms the presence of feathers of modern aspect in this key theropod group. Furthermore, the presence of large feathers on the tail, forelimbs and hindlimbs in Jianianhualong confirms that these feathering characteristics were widely present in basal paravians54. This provides a robust troodontid basis for deepening understanding of the aerodynamic capabilities of this condition, beyond the gliding-related insights from the Jehol dromaeosaurid Microraptor55,56.

The tail frond of Jianianhualong preserves an asymmetrical feather (as well as some suspected ones), the first example of feather asymmetry in troodontids. Feather asymmetry has been suggested to be closely correlated with flight capability57,58, and although the functional implications of feather asymmetry have been debated59,60, the appearance of asymmetrical vanes in flight feathers has been considered to represent one of the major events in feather evolution1,19,61. Previously, arm feathers with asymmetrical vanes have been reported only in the Microraptorinae, a dromaeosaurid subgroup, among non-avialan theropods62,63. The discovery of tail feathers with asymmetrical vanes in a troodontid theropod adds significant new information on feather evolution. Assuming the most parsimonious explanation in which feather asymmetry only evolved once, this first troodontid record suggests that feather asymmetry was ancestral to Paraves. If feather asymmetry evolved across Paraves convergently, perhaps a less likely scenario, it would still have originated amongst basal paravians. However, both scenarios indicate a higher degree of aerodynamic prowess among basal paravians than has been previously appreciated, potentially to the benefit of locomotion be it on the ground (possibly), up inclines or through the air64. In Anchiornis huxleyi, Eosinopteryx brevipenna, Jinfengopteryx elegans and Xiaotingia zhengi, the arm feathers have apparently symmetrical vanes on either side of the rachis, but this cannot be determined in Jianianhualong. In contrast, only the tail feathers of Jianianhualong are sufficiently preserved to determine their vane symmetry18,65. Parsimony-based ancestral state reconstruction of paravian arm and tail feather symmetry supports a paravian ancestor with asymmetrical arm feathers (Fig. 6; Supplementary Fig. 1). The analysis also supports asymmetrical tail feathers as the ancestral condition of a more inclusive paravian clade that excludes Scansoriopterygidae and Avialae (Fig. 6; Supplementary Fig. 1). However, it should be noted that entering the ancestral scansoriopterygid and dromaeosaurid condition as equivocal for both the arm and tail feathers results in an equivocal reconstruction at the paravian node for arm feather symmetry, but reconstructs asymmetry for the tail feathers. Thus, future fossil finds and new information revealed from existing fossils using novel observational techniques such as LSF imaging (Fig. 5f; Figs 3 and 4 of ref. 52) will be crucial towards developing a robust understanding of the pattern and significance of paravian vane asymmetry evolution. Asymmetry in the width of the leading and trailing edge vanes (Fig. 5c,d) in the tail feathers of Jianianhualong appears to have enabled the animal to dynamically maintain feather stability, or pitch in airflow66,67 (with the low angle of the leading edge barbs (10°) providing increased vane rigidity68). This aerodynamic capability may have benefited the locomotion of Jianianhualong on the ground and/or through the air, although in isolation these two aforementioned characteristics do not support either passive gliding or active, powered flight60. The tail feathers of Jianianhualong also have small trailing edge barb angles (15°), so are distinct from aerodynamically modern primary feathers60. It is worth noting that the subtriangular outline of the tail frond69,70 and the wing-like slots between its outward-curving feathers may have been helpful towards drag reduction when the tail was used69,70. In having ancestrally asymmetrical vane-widths, the feathers of early paravians (Supplementary Fig. 1) may have been similarly selected upon for improvements to locomotion along the ground and/or through the air. However, the complex factors controlling asymmetrical development in living birds71 suggests that this hypothesis requires further testing and development.

It is worth hypothesizing that Jianianhualong and other troodontids are likely to have tail feathers with asymmetrical vanes, but arm feathers with symmetrical vanes. This suggests that feather asymmetry first evolved in the troodontid tail and then appeared in other parts of the body. This hypothesis could potentially apply to paravians more generally, though not supported by our parsimony-based ancestral state reconstruction of paravian arm and tail feather symmetry (Supplementary Fig. 1). However, given the controversies over maniraptoran phylogeny, this significant feature deserves further investigation and is potentially important for our understanding of the evolution of flight feathers from both morphological and functional perspectives.

The discovery of Jianianhualong adds to the diversity of the Jehol troodontids (Fig. 7). Previously, four troodontid species have been reported from the Lower Cretaceous Jehol Group: Mei long10, Sinovenator changii9, Sinusonasus magnodens11 and Jinfengopteryx elegans13. Jianianhualong and Sinusonasus are more crownward than Mei and Sinovenator, and they display many features shared with more derived troodontids but absent in basal troodontids. This indicates the presence of a diversity of troodontids in the Early Cretaceous Jehol Group.

Figure 7: Life reconstruction of Jianianhualong tengi.
Figure 7

Life reconstruction of Jianianhualong tengi by Julius T. Csotonyi based on the holotype DLXH 1218.

Jianianhualong possesses a mosaic of plesiomorphic and apomorphic morphological features throughout the skeleton, as in troodontids such as Jinfengopteryx53. However, in Jianianhualong this mosaic displays a distinct spatial organization, which suggests that character evolution occurred regionally rather than randomly throughout its skeleton.

Comparison of morphological features of Jianianhualong with those of other troodontids indicates that Jianianhualong has forelimbs and a pelvis closely resembling those of basal troodontids, but a cranium and hindlimbs that are more similar to those of derived troodontids. For example, Jianianhualong has forelimb and pelvic features seen in basal troodontids such as Anchiornis and Sinovenator29,30 that include a humerus with a long inner tuberosity and short deltopectoral crest (also present in basal dromaeosaurids), a long manus (also present in some basal deinonychosaurs such as Yixianosaurus), a proportionally long metacarpal II, a proportionally long manual phalanx III-2 (longer than metacarpal III), a relatively small ilium (also in other basal paravians) with a wide, deep and slightly posteriorly directed pubic peduncle (also present in other derived maniraptorans), a posteroventrally oriented pubis, a pubic apron in which the dorsal portion along the midline extends posteriorly to form a longitudinal ridge, and a short ischium with posterodorsal and posteroventral processes and a distally positioned obturator process (also present in other basal paravians). Many cranial and hindlimb features of Jianianhualong are similar to those of derived troodontids, including a maxillary ascending process that extends posteriorly considerably beyond the anterior border of the antorbital fenestra, a dorsoventrally deep jugal ramus of the maxilla, a deep and narrow groove on the lateral surface of the jugal ramus of the maxilla, a sub-triangular antorbital fossa with a dorsally displaced anterior margin, the lack of a laterally visible promaxillary fenestra, an anteroventrally positioned maxillary fenestra that is significantly elongate anteroposteriorly, a relatively short jugal process of the postorbital, a proportionally long pedal digit I (phalanges I-1 and I-2 combined/metatarsal III length ratio 0.31), a metatarsal I positioned three-fifths of the way down the metatarsus, a proportionally long pedal phalanx I-1, a metatarsal II that is much more slender and considerably shorter than the other two main metatarsals, and a highly specialized pedal digit II as indicated by phalanx II-1 being 150% the length of phalanx II-2, pedal phalanx II-2 having a proximoventral heel extending considerably both proximally and ventrally, and a pedal phalanx II-3 that is much larger than pedal phalanges II-1 and II-2.

The only other Jehol troodontid to display a mosaic of plesiomorphic and apomorphic osteological features is Sinusonasus magnodens, another troodontid with transitional anatomical features (Fig. 6). Sinusonasus has a cranium that closely resembles those of basal rather than derived troodontids and a pelvis and hindlimbs that are more similar to those of derived troodontids than to basal ones. For example, pelvic and hindlimb similarities shared by Sinusonasus and derived troodontids include an anteroventrally oriented pubis, an elongate ischium with an obturator process at mid-length of the bone, and an arctometatarsalian metatarsus. Its cranial features that are shared with basal troodontids include a proportionately large and posteriorly extended external naris, a laterally exposed promaxillary fenestra, and a relatively short groove housing vascular foramina on the lateral surface of the dentary.

Natural selection has been suggested to act on modules rather than individual characters during major evolutionary transformations72. This hypothesis is supported by recent discoveries such as the pterosaur Darwinopterus, which has anatomical features that are transitional between the long-tailed basal pterosaurs and derived pterodactyloid pterosaurs73. Troodontidae is a small theropod family displaying a relatively low morphological disparity, but the distinct spatial organization of the mosaic of plesiomorphic and apomorphic osteological features in Jianianhualong and Sinusonasus raises the possibility of modular evolution in troodontids. The mosaic of plesiomorphic and apomorphic osteological features identified in Jianianhualong and Sinusonasus appears to show some correspondence to the expression domains of Hox genes, which more closely match the phenotypic modules identified in pterosaurs73. It seems possible that a functional driver may be involved in the mosaic of plesiomorphic and apomorphic osteological features identified in Jianianhualong and Sinusonasus, given their restriction to the skull, forelimbs and hindlimbs. In investigating the possibility of this driver, as well as the genetic regulation of these mosaics of plesiomorphic and apomorphic osteological features, there is a valuable opportunity to compare and contrast the mode of evolution along the different paravian lineages, particularly in understanding the early evolution of birds where aspects of previously proposed modular evolution have been supported74. Admittedly, the mosaic of plesiomorphic and apomorphic osteological characters observed here does not have as clear a distribution into modules as in species with transitional anatomical features that belong to major evolutionary transitions. For example, the cranium of Jianianhualong also displays some features seen in basal troodontids but absent in derived troodontids, including the large antorbital fenestra (as in Sinovenator, more than half the size of the antorbital fossa, and unlike the proportionally smaller one in Byronosaurus), a long maxillary tooth row terminating close to the posterior end of the jugal ramus of the maxilla, and most teeth have only relatively fine posterior serrations. The forelimb of Jianianhualong is similar to derived troodontids in being short and slender, and pedal digit II of Sinusonasus magnodens is not as specialized as in derived troodontids. Therefore, it remains a possibility that the mosaic of plesiomorphic and apomorphic osteological characters observed here, despite their distinct spatial organization, might have been produced by independent evolution of characters rather than the concerted evolution of characters within modules. Therefore, further study of mosaics of plesiomorphic and apomorphic osteological characters across paravians will be crucial towards deepening understanding of evolution along the different paravian lineages.

Methods

Material and its provenance and access

DLXH 1218 is the holotype of Jianianhualong tengi and comprises of a nearly complete skeleton with associated feathers (Fig. 1). It was collected in Lower Cretaceous Yixian Formation rocks12 of Baicai Gou (), Yixian County, western Liaoning, China and is housed at the Dalian Xinghai Museum, Liaoning in accordance with local regulations. The specimen is available for public viewing and scientific study.

Fossil excavation and preparation

Jianianhualong tengi DLXH 1218 was excavated and prepared using standard methods. Excavation involved geological hammers, chisels and protective goggles and gloves. Preparation was carried out using pneumatic tools in a preparation chamber fitted with a stereomicroscope.

Comparative anatomical studies

Standard comparative anatomy methods were used to study the new specimen. This involved comparing Jianianhualong with other fossil specimens either through first-hand studies (using a stereomicroscope to examine finer details) or literature-based studies.

Laser-stimulated fluorescence imaging

LSF imaging helped to clarify preserved feathering and osteology and confirmed the specimen’s authenticity52. The imaging involved using a custom-built laser module (405 nm 500 mw laser) to illuminate the specimen with a raster-scanned laser line (produced by a Laserline Optics Canada lens) and then capturing the fluorescence produced in a long exposure shot, using a Nikon D610 DSLR camera fitted with a laser-blocking filter. For details of the LSF protocol see refs 52, 75.

Phylogenetic analysis

The phylogenetic analysis was performed in the TNT software package76 using a recently published dataset for coelurosaurian phylogeny77 with Jianianhualong and Sinusonasus added in. This was run using a traditional search strategy with default settings other than setting the maximum trees in memory at 300,000 with 1,000 replications. Figure 6 shows the strict consensus of 3,066 most parsimonious trees (tree length=1,426, CI=0.279) produced by the analysis.

Ancestral state reconstructions

Ancestral state reconstructions for paravian arm and tail feather symmetry were performed in the evolutionary analysis software Mesquite78 using the program’s ‘parsimony ancestral state reconstruction method’ and the tree topology presented in Fig. 6.

Measurement information

Deltopectoral crest length is measured from the proximal extremity of the humerus to the summit point of the deltopectoral crest.

Nomenclatural acts

This published work and the nomenclatural acts it contains have been registered in ZooBank, the proposed online registration system for the International Code of Zoological Nomenclature. The ZooBank life science identifiers can be resolved and the associated information viewed by appending the life science identifiers to the prefix http://zoobank.org/. The life science identifiers for this publication are urn:lsid:zoobank.org:pub:1549DC59-3192-4834-9271-A8DB610969E1 and urn:lsid:zoobank.org:act:75D21EFF-2A12-45DE-85F9-7EABAF8CC6D3.

Data availability

The data reported in this paper are detailed in the main text and in the Supplementary Information.

Additional information

How to cite this article: Xu, X. et al. Mosaic evolution in an asymmetrically feathered troodontid dinosaur with transitional features. Nat. Commun. 8, 14972 doi: 10.1038/ncomms14972 (2017).

Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

References

  1. 1.

    et al. An integrative approach to understanding bird origins. Science 346, 1253293 (2014).

  2. 2.

    The origin and early evolution of birds: discoveries, disputes, and perspectives from fossil evidence. Naturwissenschaften 91, 455–471 (2004).

  3. 3.

    et al. A Jurassic avialan dinosaur from China resolves the early phylogenetic history of birds. Nature 498, 359–362 (2013).

  4. 4.

    , & A review of dromaeosaurid systematics and paravian phylogeny. Bull. Am. Mus. Nat. Hist. 371, 1–206 (2012).

  5. 5.

    , , , & New dromaeosaurids (Dinosauria: Theropoda) from the Lower Cretaceous of Utah, and the evolution of the dromaeosaurid tail. PLoS ONE 7, e36790 (2012).

  6. 6.

    , , & An Archaeopteryx-like theropod from China and the origin of Avialae. Nature 475, 465–470 (2011).

  7. 7.

    , , & A pre-Archaeopteryx troodontid from China with long feathers on the metatarsus. Nature 461, 640–643 (2009).

  8. 8.

    , , & Gradual assembly of avian body plan culminated in rapid rates of evolution across the dinosaur-bird transition. Curr. Biol. 24, 2386–2392 (2014).

  9. 9.

    , , , & A basal troodontid from the Early Cretaceous of China. Nature 415, 780–784 (2002).

  10. 10.

    & A new troodontid from China with avian-like sleeping posture. Nature 431, 838–841 (2004).

  11. 11.

    & A new troodontid (Theropoda: Troodontidae) from the Lower Cretaceous Yixian formation of western Liaoning, China. Acta Geol. Sin. (English Edn) 78, 22–26 (2004).

  12. 12.

    et al. Further support for a Cretaceous age for the feathered-dinosaur beds of Liaoning, China: new 40Ar/39Ar dating of the Yixian and Tuchengzi formations. Chin. Sci. Bull. 46, 2009–2013 (2001).

  13. 13.

    et al. First avialian bird from China. Geol. Bull. China 24, 197–210 (2005).

  14. 14.

    et al. On the horizon of Protopteryx and the early vertebrate fossil assemblages of the Jehol Biota. Chin. Sci. Bull. 53, 2820–2827 (2008).

  15. 15.

    et al. A new feathered maniraptoran dinosaur fossil that fills a morphological gap in avian origin. Chin. Sci. Bull. 54, 430–435 (2009).

  16. 16.

    et al. Reduced plumage and flight ability of a new Jurassic paravian theropod from China. Nat. Commun. 4, 1394 (2013).

  17. 17.

    & Avian Ancestors—A Review of the phylogenetic Relationships of the Theropods Unenlagiidae, Microraptoria, Anchiornis and Scansoriopterygidae Springer (2013).

  18. 18.

    , & New specimen of Archaeopteryx provides insights into the evolution of pennaceous feathers. Nature 511, 79–82 (2014).

  19. 19.

    & The origin and early evolution of feathers: insights from recent paleontological and neontological data. Vertebrata PalAsiatica 47, 311–329 (2009).

  20. 20.

    Archaeopteryx—the Icon of Evolution Verlag Dr. Friedrich Pfeil (2009).

  21. 21.

    Classification of the Dinosauria. Am. J. Sci. (ser. 3) 18, 81–86 (1881).

  22. 22.

    Bemerkungen zur Systematik und Stammesgeschichte einiger Reptilien [Remarks on the systematics and phylogeny of some reptiles]. Zeitschrift für Induktive Abstammungsund Vererbungslehre 22, 209–212 (1920).

  23. 23.

    Saurischian monophyly and the origin of birds. Mem. Calif. Acad. Sci. 8, 1–55 (1986).

  24. 24.

    On Troodon validus, an ornithopodous dinosaur from the Belly River Cretaceous of Alberta, Canada. Bull. Dept. Geol. Univ. Alb. 1, 1–43 (1924).

  25. 25.

    Axial skeletal ontogeny in the Parasuchia (Archosauria: Pseudosuchia) and its implications for ontogenetic determination in Archosaurs. J. Vertebr. Paleontol. 27, 350–361 (2007).

  26. 26.

    Closure of neurocentral sutures during crocodilian ontogeny: implications for maturity assessment in fossil archosaurs. J. Vertebr. Paleontol. 16, 49–62 (1996).

  27. 27.

    , , & Body mass estimation in non-avian bipeds using a theoretical conversion to quadrupedal stylopodial proportions. Methods Ecol. Evol. 5, 913–923 (2014).

  28. 28.

    A new phylogeny of the carnivorous dinosaurs. Gaia 15, 5–61 (2000).

  29. 29.

    & in The Dinosauria 2nd edn (eds Weishampel D. B., Dodson P., Osmólska H. 184–195University of California Press (2004).

  30. 30.

    Deinonychosaurian Fossils From the Jehol Group of Western Liaoning and the Coelurosaurian Evolution PhD thesis Chinese Academy of Sciences (2002).

  31. 31.

    et al. A review of the Mongolian Cretaceous dinosaur Saurornithoides (Troodontidae: Theropoda). Am. Mus. Novit. 3654, 1–63 (2009).

  32. 32.

    , , & Osteology and relationships of Byronosaurus jaffei (Theropda: Troodontidae). Am. Mus. Novit. 3402, 1–32 (2003).

  33. 33.

    & A nearly complete skeleton of a new troodontid dinosaur from the Early Cretaceous of the Ordos Basin, Inner Mongolia, People’s Republic of China. Can. J. Earth Sci. 30, 2163–2173 (1994).

  34. 34.

    , , & A new troodontid theropod dinosaur from the Lower Cretaceous of Utah. PLoS ONE 5, e14329 (2010).

  35. 35.

    et al. The taxonomic status of the Late Cretaceous dromaeosaurid Linheraptor exquisitus and its implications for dromaeosaurid systematics. Vertebrata PalAsiatica 53, 29–62 (2015).

  36. 36.

    , , , & A short-armed troodontid dinosaur from the Upper Cretaceous of Inner Mongolia and its implications for troodontid evolution. PLoS ONE 6, e22916 (2011).

  37. 37.

    , & The systematic position of the enigmatic theropod dinosaur Yixianosaurus longimanus. Vertebrata PalAsiatica 51, 169–183 (2013).

  38. 38.

    , , , & The theropod furcula. J. Morphol. 270, 856–879 (2009).

  39. 39.

    et al. A Jurassic ceratosaur from China helps clarify avian digit homologies. Nature 459, 940–944 (2009).

  40. 40.

    in The Origin and Evolution of Birds 2nd edn Yale University Press (1999).

  41. 41.

    , & The smallest known non-avian theropod dinosaur. Nature 408, 705–708 (2000).

  42. 42.

    Homology and evolution of the deep dorsal thigh musculature in birds and other Reptilia. J. Morphol. 189, 327–346 (1986).

  43. 43.

    & The affinities of a new theropod from the Alxa Desert, Inner Mongolia, People’s Republic of China. Can. J. Earth Sci. 30, 2107–2127 (1993).

  44. 44.

    , , & The theropod ancestry of birds: new evidence from the Late Cretaceous of Madagascar. Science 279, 1915–1919 (1998).

  45. 45.

    , & A dromaeosaurid dinosaur with a filamentous integument from the Yixian Formation of China. Nature 401, 262–266 (1999).

  46. 46.

    & Important features of the dromaeosaurid skeleton II: information from newly collected specimens of Velociraptor mongoliensis. Am. Mus. Novit. 3282, 1–45 (1999).

  47. 47.

    & Important features of the dromaeosaur skeleton: information from a new specimen. Am. Mus. Novit. 3215, 1–28 (1997).

  48. 48.

    Osteology of Deinonychus antirrhopus, an unusual theropod from the Lower Cretaceous of Montana. Bull. Peabody Mus. Nat. History Yale Univ. 30, 1–165 (1969).

  49. 49.

    & A juvenile specimen of Saurornithoides mongoliensis from the Upper Cretaceous of northern China. Can. J. Earth Sci. 30, 2224–2230 (1994).

  50. 50.

    et al. The taxonomy of the troodontid IVPP V10597 reconsidered. Vertebrata PalAsiatica 50, 140–150 (2012).

  51. 51.

    & Troodontid-like pes in the dromaeosaurid Sinornithosaurus. Paleontol. Soc. Korea Special Publication 2000, 179–188 (2000).

  52. 52.

    et al. Laser-stimulated fluorescence in paleontology. PLoS ONE 10, e0125923 (2015).

  53. 53.

    & Jinfengopteryx compared to Archaeopteryx, with comments on the mosaic evolution of long-tailed avialan birds. Acta Geol. Sin. 81, 337–343 (2007).

  54. 54.

    et al. Hind wings in basal birds and the evolution of leg feathers. Science 339, 1309–1312 (2013).

  55. 55.

    , , , & Model tests of gliding with different hindwing configurations in the four-winged dromaeosaurid Microraptor gui. Proc. Natl Acad. Sci USA 107, 2972–2976 (2010).

  56. 56.

    et al. Aerodynamic performance of the feathered dinosaur Microraptor and the evolution of feathered flight. Nat. Commun. 4, 2489 (2013).

  57. 57.

    & Feathers of Archaeopteryx: asymmetric vanes indicate aerodynamic function. Science 203, 1021–1022 (1979).

  58. 58.

    & Avian Anatomy: integument US Department of Agriculture (1972).

  59. 59.

    The aerodynamics of feather asymmetry and implications for paravian flight. FASEB J. 28, Supplement 14 (2014).

  60. 60.

    , & Barb geometry of asymmetrical feathers reveals a transitional morphology in the evolution of avian flight. Proc. R. Soc. B Biol. Sci. 282, 20142864 (2015).

  61. 61.

    Development and evolutionary origin of feathers. J. Exp. Zool. 285, 291–306 (1999).

  62. 62.

    et al. Four-winged dinosaurs from China. Nature 421, 335–340 (2003).

  63. 63.

    & A large, short-armed, winged dromaeosaurid (Dinosauria: Theropoda) from the Early Cretaceous of China and its implications for feather evolution. Sci. Rep. 5, 11775 (2015).

  64. 64.

    , & The wings before the bird: an evaluation of flapping-based locomotory hypotheses in bird antecedents. PeerJ 4, e2159 (2016).

  65. 65.

    On the identification of feather structures in stem-line representatives of birds: evidence from fossils and actuopalaeontology. Paläontologische Zeitschrift 86, 91–102 (2012).

  66. 66.

    How a long tail and changes in mass and wing shape affect the cost for flight in animals. Funct. Ecol. 9, 48–54 (1995).

  67. 67.

    Feather asymmetry in Archaeopteryx. Nature 374, 221–222 (1995).

  68. 68.

    , & Functional morphology of the vanes of the flight feathers of the pigeon Columba livia. J. Exp. Biol. 198, 1219–1228 (1995).

  69. 69.

    On the tails of birds. Bioscience 47, 215–225 (1997).

  70. 70.

    Drag reduction by wing tip slots in a gliding Harris’ hawk, Parabuteo unicinctus. J. Exp. Biol. 198, 775–781 (1995).

  71. 71.

    & Theoretical morphology and development of flight feather vane asymmetry with experimental tests in parrots. J. Exp. Zool. B Mol. Dev. Evol. 322, 240–255 (2014).

  72. 72.

    The units of selection revisited: the modules of selection. Biol. Philos. 14, 167–180 (1999).

  73. 73.

    , , , & Evidence for modular evolution in a long-tailed pterosaur with a pterodactyloid skull. Proc. R. Soc. B Biol. Sci. 277, 383–389 (2010).

  74. 74.

    & Mosaicism, modules, and the evolution of birds: results from a Bayesian approach to the study of morphological evolution using discrete character data. Syst. Biol. 57, 185–201 (2008).

  75. 75.

    et al. Basal paravian functional anatomy illuminated by high-detail body outline. Nat Commun. 8, 14576 (2017).

  76. 76.

    , & TNT, a free program for phylogenetic analysis. Cladistics 24, 774–786 (2008).

  77. 77.

    et al. A bizarre Jurassic maniraptoran theropod with preserved evidence of membranous wings. Nature 521, 70–73 (2015).

  78. 78.

    Mesquite: a modular system for evolutionary analysis v. 2.7.4 (2010). Available at .

Download references

Acknowledgements

We thank Fangfang Teng for allowing us to study the specimen, Gao Xia for logistics during X.X.’s visit to the collection and Maureen Walsh for preparing the specimen. We also thank Thomas G. Kaye for his help in processing the LSF images. This study was supported by the National Science Foundation of China (41688103, 41120124002 and 91514302), the Dr Stephen S.F. Hui Trust Fund (201403173007), the Research Grant Council of Hong Kong’s General Research Fund (17103315) and the Faculty of Science of the University of Hong Kong.

Author information

Author notes

    • Xing Xu
    • , Philip Currie
    •  & Michael Pittman

    These authors contributed equally to this work.

Affiliations

  1. Key Laboratory of Vertebrate Evolution and Human Origins, Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences, Beijing 100044, China

    • Xing Xu
  2. Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2E9

    • Philip Currie
  3. Vertebrate Palaeontology Laboratory, Department of Earth Sciences, The University of Hong Kong, Pokfulam, Hong Kong

    • Michael Pittman
  4. School of the Earth Sciences and Resources, China University of Geosciences, Beijing 100083, China

    • Lida Xing
  5. Beijing Museum of Natural History, Beijing 100050, China

    • Qingjin Meng
  6. Institute of Geology, Chinese Academy of Geological Sciences, Beijing 100037, China

    • Junchang Lü
  7. Paleontological Institute & Key Laboratory for Evolution of Past Life in Northeast Asia, Ministry of Land and Resources, Shenyang Normal University, Shenyang 110034, China

    • Dongyu Hu
  8. College of Life Sciences, Peking University, Beijing 100871, China

    • Congyu Yu

Authors

  1. Search for Xing Xu in:

  2. Search for Philip Currie in:

  3. Search for Michael Pittman in:

  4. Search for Lida Xing in:

  5. Search for Qingjin Meng in:

  6. Search for Junchang Lü in:

  7. Search for Dongyu Hu in:

  8. Search for Congyu Yu in:

Contributions

X.X., P.C. and M.P. designed the project, X.X., P.C., M.P., L.X., Q.M., J.L. and C.Y. performed the research, and X.X., P.C. and M.P. wrote the manuscript.

Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Xing Xu.

Supplementary information

PDF files

  1. 1.

    Supplementary Information

    Supplementary Figure, Supplementary Tables, Supplementary Note and Supplementary References

  2. 2.

    Peer Review File

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Creative Commons BYThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/