ISG15 is an interferon (IFN)-α/β-induced ubiquitin-like protein. It exists as a free molecule, intracellularly and extracellularly, and conjugated to target proteins. Studies in mice have demonstrated a role for Isg15 in antiviral immunity. By contrast, human ISG15 was shown to have critical immune functions, but not in antiviral immunity. Namely, free extracellular ISG15 is crucial in IFN-γ-dependent antimycobacterial immunity, while free intracellular ISG15 is crucial for USP18-mediated downregulation of IFN-α/β signalling. Here we describe ISG15-deficient patients who display no enhanced susceptibility to viruses in vivo, in stark contrast to Isg15-deficient mice. Furthermore, fibroblasts derived from ISG15-deficient patients display enhanced antiviral protection, and expression of ISG15 attenuates viral resistance to WT control levels. The species-specific gain-of-function in antiviral immunity observed in ISG15 deficiency is explained by the requirement of ISG15 to sustain USP18 levels in humans, a mechanism not operating in mice.
Interferon-stimulated gene 15 (ISG15) is an interferon (IFN)-α/β-inducible ubiquitin-like molecule. It has two ubiquitin-like domains and is synthesized from an immature precursor by cleavage of a C-terminal extension peptide1. Mature ISG15 has the LRLRGG sequence, which is also present at the C terminus of mature ubiquitin. ISG15 exists in two distinct states: as a free molecule (intracellular and extracellular) or conjugated to target protein lysine residues (ISGylation)2,3,4,5,6,7,8,9,10. In a process similar to ubiquitin ligation, ISGylation involves a conjugation pathway including E1 (UBE1L), E2 (UBCH8) and E3 (HERC5, EFP, HHARI) enzymes. ISGylation is reversed by the action of the isopeptidase ubiquitin-specific protease 18 (USP18; UBP43)11,12. Accordingly, cells deficient in the conjugation machinery exhibit absent or reduced ISGylation, while Usp18-deficient cells exhibit high levels of Isg15 conjugates13. All of the enzymes involved in ISGylation/de-ISGylation are transcriptionally induced by IFN-α/β.
Numerous in vivo and in vitro studies have ascribed an antiviral role to ISG15. Mice lacking Isg15 exhibit enhanced susceptibility to challenge with multiple viruses, including murine gammaherpesvirus 68 (ref. 14), influenza A virus (IAV)14, influenza B virus14,15, Sindbis virus14, vaccinia virus16, herpes simplex virus 1 (HSV-1)14, Chikungunya virus17 and murine norovirus18, yet no enhanced susceptibility to vesicular stomatitis virus (VSV) and lymphocytic choriomeningitis virus19. In vitro studies in mouse embryonic fibroblasts (MEFs), mouse lung fibroblasts and RAW 264.7 cells have demonstrated an antiviral role for Isg15 during Sindbis virus20, vaccinia virus16, Ebola virus21, Dengue virus and West Nile virus22 infection, although there are several reports of viruses exhibiting no enhanced replication in the absence of Isg15 (murine gammaherpesvirus 68, HSV-1, Sindbis virus, IAV14). ISG15 small interfering RNA (siRNA) knockdown in human cells has also suggested an antiviral role for free and/or conjugated ISG15 during infection with numerous viruses, including IAV23, human immunodeficiency virus24,25,26, Ebola virus21,27, human papilloma virus28, VSV27, Japanese encephalitis virus15 and Sendai virus (SeV)29. While these many studies have suggested an antiviral role for ISG15, others have suggested no role at all; the one clear exception is hepatitis C virus (HCV). In vitro studies of HCV infection suggest that ISG15 exhibits proviral activity30,31. The silencing of ISG15 in human hepatoma cells was found to lead to an inhibition of HCV replication, increases in cell sensitivity to IFN-α/β and accumulation of IFN-α/β-stimulated gene products (ISGs).
Our studies on ISG15-deficient individuals have demonstrated a critical role for free extracellular ISG15 in the induction of IFN-γ and protection against mycobacterial disease. These findings were confirmed in Isg15-deficient mice9. Intriguingly, there is no evident overlap in viral susceptibility phenotype between mice and humans lacking ISG15. Despite being serologically positive for numerous childhood viruses, such as human cytomegalovirus (HCMV), Epstein Barr virus, measles virus, varicella-zoster virus, HSV-1 and -2, mumps virus, IAV and hepatitis A virus, ISG15-deficient individuals exhibit no overt susceptibility to viral disease9,10. We recently demonstrated that human free intracellular ISG15 binds USP18, a negative regulator of IFN-α/β signalling. This binding prevents the SKP2-mediated degradation of USP18, and thus is critical for the accumulation of USP18 and the appropriate regulation of IFN-α/β signalling. Indeed, patients lacking ISG15, and consequently having low USP18 levels, display higher steady-state levels of ISG transcripts in whole blood than controls10,32.
We demonstrate that human cells deficient in ISG15 exhibit a persistent elevation in ISG expression. We find that, not only do humans deficient in ISG15 lack any overt susceptibility to viral infection, but cells derived from these individuals exhibit an enhancement of broad protection against viruses. Complementation of ISG15-deficient cells with the wild-type (WT) or conjugation-deficient ΔGG ISG15 alleles rescues both WT levels of ISG expression and viral susceptibility. Furthermore, we demonstrate that there is a species-specific interaction between ISG15 and USP18. While humans require ISG15 for USP18 stability and appropriate negative regulation of the IFN response, mice lack this stable interaction and can regulate the IFN response in the absence of Isg15.
Persistent increase of ISG expression in cells lacking ISG15
The ISG15-deficient individuals identified to date have been infected with numerous childhood viruses, against which they mounted a humoral immune response9,10. No overt viral illnesses were documented in these patients, suggesting that ISG15 may not be essential for immunity to these viruses. ISG15-deficient patients may actually have a milder disease course than wild-type individuals. However, this is difficult to demonstrate, as information about subclinical illness is often not transmitted by patients to their doctors or noted by the doctors themselves. We tested this hypothesis in vitro using hTert-immortalized dermal fibroblasts derived from ISG15-deficient patients and relevant WT controls. Human cells lacking ISG15 also have low USP18 levels (due to the lack of USP18 stabilization by ISG15) and high levels of ISG transcripts, as documented ex vivo with leukocytes from ISG15-deficient individuals10. We carried out in vitro studies, monitoring the kinetics and persistence of ISG induction by quantitative real-time PCR in WT and ISG15-deficient fibroblasts during and after IFN priming, in which the cells were pulsed with IFN-α2b for 12 h and then allowed to rest for 36 h. We determined mRNA levels for the IFN-induced protein with tetratricopeptide repeats 1 (IFIT1) and myxovirus resistance 1 (MX1), from 12 h to 6 days post-IFN-α/β priming. The expression levels of IFIT1 and MX1 transcripts during peak induction and early dampening were similar between ISG15-deficient and control cells (Fig. 1a). However, the time taken for IFIT1 transcript levels to return to baseline after IFN priming differed between control and ISG15-deficient cells. Specifically, IFIT1 transcript levels remained significantly elevated for more than 5 days in ISG15-deficient cells, whereas they returned to basal levels within 2 days in control cells (Fig. 1a).
ISG15-deficient IFN-primed cells are resistant to viral infection
ISG15-deficient patients had previously been infected with HSV-1, HCMV and IAV, as indicated by the results of serological tests10, but did not develop severe disease. We, therefore, investigated whether the prolonged increase in ISG levels affected the susceptibility of the patients’ cells to viral challenge. When IFN-α/β-primed ISG15-deficient fibroblasts were infected with HSV-1 (Fig. 1b), HCMV (Fig. 1c) or IAV (Fig. 1d) levels of viral replication were significantly lower in ISG15-deficient cells than in control cells. SeV, a murine paramyxovirus known to be highly sensitive to human ISGs, also replicated significantly less efficiently in IFN-α/β-primed ISG15-deficient cells (Fig. 1e). Finally, we investigated whether ISG15-deficient cells were also resistant to highly pathogenic viruses affecting humans that our patients would have been unlikely to encounter, by infecting control and ISG15-deficient fibroblasts with Rift Valley fever virus (RVFV) and Nipah virus (NiV). Both of these viruses also appeared to replicate less efficiently in IFN-α/β-primed ISG15-deficient cells than in control cells (Fig. 1f,g), although neither RVFV nor NiV reached statistical significance. Thus, the prolonged induction of ISGs observed in ISG15-deficient human cells protects against viruses from multiple families, having both RNA and DNA genomes, and causing both low and highly pathogenic disease in normal healthy humans. These results further suggest that viral infections in ISG15-deficient individuals may follow an attenuated course with milder symptoms. Thus, the in vivo clinical follow-up of ISG15-deficient individuals, serological testing and the results of in vitro studies of patient-derived cells all indicate a greater resistance to viral challenge, consistent with a proviral role of ISG15 in humans.
WT or ΔGG ISG15 allele restores WT viral susceptibility
We verified that the observed phenotype was due solely to the lack of ISG15 by stably transducing ISG15-deficient patient and control fibroblasts with lentiviral particles encoding ISG15 or control luciferase10. The cells were subsequently infected with VSV, another virus known to be sensitive to low levels of IFN-α/β. Control fibroblasts displayed similar levels of VSV replication with and without IFN-α/β priming, suggesting that most of the ISG antiviral activity had declined during the post-priming rest (Fig. 2a). By contrast, IFN-α/β-primed cells deficient in ISG15 displayed significantly lower levels of viral replication (Fig. 2a), indicating a protracted antiviral state, as confirmed with other viral challenges (Fig. 1b-g). The resistance phenotype was confirmed using fibroblasts from the three ISG15-deficient patients and five controls (Supplementary Fig. 1). Similar phenotypic differences in viral replication were observed between control and ISG15-deficient fibroblasts infected with VSV-expressing GFP (VSV–GFP), as assessed by fluorescence microscopy (Fig. 2b,c). Complementation of ISG15-deficient patient fibroblasts with WT ISG15, but not with control luciferase, restored both ISG expression (Fig. 2d) and viral replication (Fig. 2e) to the levels observed in the control cells. We have previously shown that the conjugation-deficient mutant of ISG15 (ISG15ΔGG) retains the ability to bind and stabilize USP18, and is thus a functional negative regulator of IFN signalling10. Accordingly, we used ISG15-deficient fibroblasts transduced with ISG15ΔGG, which exhibit fully functional downregulation of IFN signalling but no ISGylation, to determine the relative role of each function during viral infection. As observed with WT ISG15, complementation with ISG15ΔGG rescued both the ISG expression and VSV replication phenotypes in ISG15-deficient fibroblasts (Fig. 2d,e). Similar results were obtained in A549 and Hela cells that were silenced with siRNA targeting ISG15 or a non-silencing control. In both cell lines, knockdown of ISG15 enhanced ISG expression and resistance to viral replication (Supplementary Fig. 2a-f). These data demonstrate the dependence of the in vitro phenotype on the ISG15 allele and suggest that, in human epithelial cells, the dominant role of ISG15 during viral replication is the ISGylation-independent downregulation of IFN-α/β signalling.
Isg15-deficient mice do not exhibit elevated ISG expression
All of our observations, whether from the clinical history of ISG15-deficient patients or from in vitro studies of ISG15-deficient patient-derived cells, point to a lack of overt susceptibility to viruses, and suggest that ISG15 may exhibit a proviral role. We were unable to reconcile these data with the numerous reports ascribing a critical antiviral role to Isg15 during the infection of mice in vivo with multiple viruses (for example, chikungunya virus17, murine gammaherpesvirus 68 (ref. 14), IAV14, influenza B virus14,33, Sindbis virus14, vaccinia16, HSV-1 (ref. 14) and murine norovirus18). We, therefore, analysed whole blood from Isg15-deficient mice, to determine whether these mice, like ISG15-deficient humans, also displayed elevated levels of steady-state ISG expression in the absence of diagnosable infection10. In unstimulated conditions, Ifit1 mRNA levels of Isg15-deficient animals were similar to those of their age-matched WT cagemates (Fig. 3a). This indicates a species-specific difference in ISG regulation in the absence of ISG15. We then hypothesized that, in natura, humans are constantly exposed to environmental challenges that induce IFN-α/β, resulting in persistently high levels of ISGs in ISG15-deficient individuals, due to a lack of negative regulation. Our mice were not housed in completely sterile conditions, but were nevertheless somewhat shielded from true environmental exposure to microbes. IFN-α/β induction was mimicked by injecting 10,000 U of type-I IFN intraperitoneally into WT control and Isg15-deficient mice, followed by analysis of the levels of ISG transcripts in whole blood at multiple timepoints. As expected, the animals receiving IFN injections displayed a robust increase in ISG expression within 8 h that was fully resolved within 24 h. However, no difference in ISG expression levels between Isg15-deficient and WT mice was observed at any time point (Fig. 3b–d). For further comparison with our results in human cells, primary MEFs derived from both WT and Isg15-deficient mice were primed with type-I IFN for 12 h, allowed to rest for 36 h and the levels of Ifit1 mRNA were analysed. No differences in Ifit1 expression levels were observed between WT and Isg15-deficient cells at any time point after IFN priming (Fig. 3e). These experiments suggest that the impact of ISG15 on IFN-α/β-induced signalling may indeed differ between humans and mice.
Isg15 does not sustain Usp18 levels in mice
Due to the suboptimal levels of USP18, IFN-α/β signalling persists and ISG transcripts accumulate in ISG15-deficient human cells10. We, therefore, investigated whether late IFN-α/β signalling and Usp18 levels were also affected in Isg15-deficient murine cells. WT and Isg15-deficient MEFs were subjected to prolonged stimulation with IFN-β, and the levels of phosphorylated Stat2 and IFN-stimulated proteins were measured by western blotting. In contrast to what was observed for human fibroblasts, WT and Isg15-deficient MEFs accumulated similar levels of phosphorylated Stat2, Ifit2 and Usp18 over the time course of the experiment (Fig. 4a). The IFN-α/β response was also studied in primary bone marrow-derived macrophages (BMM) from WT, Isg15-deficient or Ube1L-deficient (free Isg15 present, but no ISGylation) mice. No major differences were observed between the three strains, indicating that neither free, nor conjugated Isg15 was required to stabilize Usp18 in mice (Fig. 4b). Similar conclusions were drawn from studies of a murine fibroblast cell line (LL171), in which Isg15 was silenced with siRNA (Fig. 4c). These results provide strong evidence to suggest that, in the mouse system, Isg15 is not involved in the Usp18-mediated negative feedback loop.
USP18 stabilization by ISG15 is species-specific
The unique properties of the USP18 or ISG15 orthologues, or of both of them, may underlie the observed species-specific functional differences. ISG15 is weakly conserved across species34, whereas the murine and human USP18 proteins are 70.1% identical and 79.7% similar. We confirmed that human ISG15 stabilizes USP18 through ISGylation-independent binding10, since the conjugation-deficient ISG15ΔGG mutant was able to stabilize USP18 (Fig. 5a). Furthermore, co-expression of USP18 with an unrelated protein (GFP) did not sustain USP18 levels (Fig. 5b). The ISG15:USP18 complex could be detected by co-immunoprecipitation (co-IP; Fig. 5c, lane 2) and was abolished in the presence of N-ethylmaleimide (NEM), which alkylates cysteine residues (Fig. 5c, lane 3). Yet, the ISG15:USP18 complex retained NEM sensitivity even when we used the USP18-C64S catalytic mutant, suggesting a role for cysteine residues outside of the catalytic core (Fig. 5d).
Next, we tested whether murine Isg15:Usp18 complexes could be detected by co-IP. Weak or completely absent interactions between the two murine proteins was detected in co-IP assays (Fig. 5e). However, USP18 is the cognate deISGylase both in humans and mice. To further examine this interaction we used a micropatterning approach to quantify the stability of the ISG15:USP18 complex in living cells35. In this experimental setup, the murine Usp18:Isg15 complex was observed, but it exhibited reduced stability when compared to the human complex (Supplementary Figs 3 and 4, Supplementary Movie 1 and Supplementary Methods). The controls for the micropatterning and fluorescence recovery after photobleaching experiments are outlined in Supplementary Fig. 4. Next, we tested whether transient co-expression of murine Isg15 and Usp18 influenced the levels of murine Usp18. Murine Isg15 failed to sustain the level of murine Usp18 (Fig. 5f–h), in contrast to the phenotype observed with the human orthologues. The inability of overexpressed murine Isg15 to sustain murine Usp18 is consistent with our functional data on the endogenous murine proteins (Fig. 4) and with the comparable downregulation of IFN-α/β responses in WT and Isg15-deficient mice in vivo (Fig. 3a–d). Altogether, these results indicate that the difference in viral susceptibility between ISG15-deficient humans and mice stems from the need for ISG15 to stabilize USP18, and thus downregulate the IFN-α/β response, in humans but not in mice.
IFN-α/β signalling and ISG induction are controlled at multiple levels by negative regulators, such as SOCS (suppressor of cytokine signalling), PIAS (protein inhibitor of activated STAT), protein phosphatases (for example, SHP1) and USP18 (refs 36, 37, 38). However, we have shown that these regulators are not sufficient, and that ISG15 is required for complete downregulation of IFN-α/β signalling (Fig. 1a). Humans lacking ISG15 consistently display high steady-state levels of ISGs in whole blood, and in patient-derived fibroblasts following priming with IFN-α/β (ref. 10). We hypothesized that this dysregulation of IFN-α/β signalling would account for the lack of an overt viral susceptibility in humans, in contrast to what was observed in mice. We show here that, unlike the antiviral activity of Isg15 observed in mice, human ISG15 promotes a proviral state following IFN priming. The persistent ISG expression observed in human ISG15-deficient cells imparts a resistance to infection with multiple families of viruses with both RNA and DNA genomes.
These findings contrast from previous reports that have ascribed an antiviral role to ISG15 in human cells. Shi et al.29 reported that, following silencing of ISG15 for 48 h in HEK-293 cells, SeV titres increased by ∼0.5 and ∼1.0 log at 6 h and 12 h post infection, respectively. Tang et al.23 found that following ISG15 silencing, A549 cells became ∼fivefold more susceptible to infection by IAV (A/PR/8/1934). While these studies point to an antiviral role of ISG15 in human cells, our experiments with SeV and IAV do not capture the small observed difference in viral growth when comparing WT and ISG15-deficient hTert-immortalized fibroblasts without IFN priming (Fig. 1). These studies focused on early timepoints of the acute IFN response induced by the virus, and did not capture the more powerful role of ISG15 as a negative regulator of IFN signalling. Even if there may be a small antiviral role for ISG15 during the acute IFN response, it is clinically irrelevant in vivo and not readily captured in vitro.
Interestingly, we observed that the conjugation-deficient ISG15ΔGG was as efficient as WT ISG15 in rescuing viral replication in ISG15-deficient patient cells. One could have predicted an even better rescue of both the ISG expression and infection phenotypes by ISG15ΔGG, since the latter should be more readily accessible to mediate USP18 stability than the conjugation-competent form of ISG15. However, this was not the case, suggesting the possibility that ISG15ΔGG may have lower USP18-binding affinity or that only a small amount of ISG15 is required to stabilize USP18, and the increased availability of ISG15ΔGG has no further effect. An additional possibility, albeit less likely given the depth of work demonstrating the antiviral role of ISGylation, is that ISG15 in the conjugated form may also exhibit some proviral properties.
We have shown that free ISG15 associates with higher affinity to USP18 in humans as compared with mice, although the species-specific structural determinants involved in complex formation have yet to be identified. Another species-restricted complex involving free ISG15 has been described. The NS1 protein of influenza B virus binds non-covalently to human and non-human primate ISG15, but not to murine Isg15 (refs 39, 40, 41). Mutational and structural studies of the NS1:ISG15 complex have shown that the molecular determinant of the binding specificity (humans versus mice) lies in the small hinge region of ISG15 (refs 42, 43). Further work is required to identify the domain(s) responsible for the human ISG15:USP18 interactions that are required to enhance stability and properly regulate the IFN responses.
Unlike humans, mice can downregulate IFN signalling regardless of ISG15 competency and it is currently unknown whether murine Usp18 is stabilized by another protein, IFN-stimulated or otherwise. Individuals lacking ISG15 exhibit elevated ISG expression, which may confer an enhanced protection against viral infection, but do not exhibit any of the side-effects associated with IFN treatment10. As such, the USP18:ISG15 interaction is a potentially attractive target for small molecule-based short-term treatments to boost endogenous IFN activity.
Our findings raise interesting questions about the evolutionary role of ISG15. Given the detrimental effects that inflammation may cause in species with a longer lifespan, gain-of-function binding mutations in ISG15 and/or USP18 may reflect an evolutionary necessity in humans, and likely other mammals, to more stringent tuning of the IFN system. On the other hand, viruses have developed immune evasion strategies in parallel. Indeed, many human viral pathogens were shown to oppose ISGylation (influenza B virus NS139 and vaccinia virus E3L16,44 proteins) or induce de-ISGylation (numerous viral L proteins containing ovarian tumour45 or papain-like protease46). Much of the work characterizing these viral proteins has focused on the inhibition/removal of conjugated ISG15 from viral and host targets. However, in light of our data demonstrating that the primary role of human ISG15 is to stabilize USP18 and downregulate the IFN response, it may be that inhibition of ISGylation and removal of conjugated ISG15 is a novel mode of IFN antagonism. By enhancing the pool of free ISG15, these viral proteins may enhance USP18 levels, dampen IFN signalling and promote viral escape.
Based on current knowledge, murine Isg15 acts in an antiviral manner (largely via ISGylation), while murine Usp18 functions in a proviral manner (IFN negative regulation and de-ISGylation). By contrast, human ISG15 and USP18 both appear to function in an anti-inflammatory manner. Consequently, this makes them proviral, although the ISGylation of cellular or viral proteins may play a non-dominant antiviral role. However, if both ISGylation and de-ISGylation are proviral (through provision of the free ISG15 required for USP18 stabilization), this notion becomes somewhat paradoxical. In humans then, this process may serve as a mechanism for the temporal regulation of IFN-α/β-induced signalling. We suggest that one of the roles of ISGylation at early stages of viral infection in humans is to sequester free ISG15, thereby allowing IFN signalling to occur. At later stages, USP18-mediated de-ISGylation releases free ISG15, which in turn stabilizes USP18 and tunes down IFN-α/β signalling and inflammation.
Icahn School of Medicine IRB board has approved the use of human subject cell lines. Dermal fibroblasts from ISG15-deficient patients (n=3) and WT controls (n=5) were immortalized by stable transduction with hTert10. Patient (n=3) and control (n=2) cells were stably complemented with ISG15, ISG15ΔGG or luciferase by lentiviral transduction10. Transduced fibroblasts were sorted on the basis of RFP expression (BD FACS Aria II). The stable expression of the transduced constructs was periodically checked by western blotting. Murine embryonic fibroblasts from WT C57BL6/J and Isg15-deficient mice were derived by disaggregation of day 13.5 embryos from timed matings by trituration of the embryos at 37 °C in trypsin19. Mouse bone marrow macrophages (BMM) were generated by isolating bone marrow cells capable of adhering to non-tissue-culture treated plates for 7 days in BMM media (Dulbeco’s Modified Eagle Medium, 10% fetal calf serum, 5% horse serum, 2% supernatant derived from CMG14-12 cells)47. LL171 cells (clonal isolate of murine L929 fibroblasts; from G. Uzé, CNRS, Montpellier, France), HEK293T (CRL-11268; ATCC), Vero-E6 (CRL-1586; ATCC), Vero (CCL81; ATCC), HeLa (CCL-2; ATCC), A549 (CCL-185; ATCC), murine embryonic fibroblasts and human fibroblasts were cultured in normal growth medium consisting of DMEM supplemented with 10% fetal calf serum. All cells were cultured at 37 °C and 10% CO2. All cells were tested for mycoplasma contamination using the PlasmoTest kit according to the manufacturer instructions (Invivogen).
Untransduced, luciferase-, ISG15-, and ISG15ΔGG-transduced fibroblasts (Figs 1, 2, 3) were primed by incubation with 0, 10, 100, or 1,000 IU ml−1 IFN-α2b (Merck IntronA) in normal growth medium for 12 h. The IFN-α2b was then eliminated by thorough washing with PBS and the cells were allowed to rest for 36 h in normal medium before infection. Murine embryonic fibroblasts were primed with 1,000 U universal type-I IFN (Fig. 3; PBL IFN) or 500 pM murine IFN-β (Fig. 4; PBL IFN). Mouse bone marrow macrophages were primed with 250 pM murine IFN-α4 (Fig. 4; Calbiochem). LL171 cells were primed with 10 pM murine IFN-β (Fig. 4; PBL IFN).
ISG Expression analysis
RNA was extracted from fibroblasts (Qiagen RNeasy) or whole blood (PAXgene Blood RNA Kit) and reverse-transcribed (ABI High Capacity Reverse Transcriptase) according to the instructions provided by the manufacturer. Levels of ISG expression (IFIT1, MX1), relative to the 18S rRNA housekeeper gene, were analysed by Taqman quantitative real-time PCR (TaqMan Universal Master Mix II w/ UNG, Roche LightCycler 480 II). The relative expression levels of the ISGs were calculated by the ΔΔCT method, with comparison with the mean value for mock-treated controls.
All infections were performed 48 h after IFN treatment (12 h priming, 36 h rest) in normal growth medium. The viruses and multiplicities of infection used were as follows: VSV Indiana strain at an MOI of 1.0, rVSV–GFP Indiana strain48 at an MOI of 10.0, rHSV-1 US11-GFP Patton strain49 at an MOI of 1.0 (kindly provided by the laboratory of Ian Mohr), rHCMV IE2-YFP strain AD169 (ref. 50) at an MOI of 2.0, rIAV A/PR/8/34 gLuc virus51 modified by the introduction of a PTV1 2A cleavage site (PR8-gLuc-PTV1; kindly provided by Nick Heaton) at an MOI of 10.0, rSeV-eGFP Fushimi strain52 modified by the introduction of a gLuc-P2A cleavage site (rSeV-gLuc-P2A-eGFP; same method as for NiV below) at an MOI of 0.1, RVFV strain ZH501 at an MOI of 0.01, and the rNiV-gLuc-P2A-eGFP Malaysian strain52 at an MOI of 0.01. All work with infectious RVFV and rNiV was carried out under biosafety level 4 (BSL-4) conditions at the Robert E. Shope BSL-4 laboratory at the University of Texas Medical Branch.
Analysis of viral replication
VSV-infected supernatants were removed 24 h.p.i. and titered by determining the 50% tissue culture infectious dose on Vero-E6 cells. RVFV and rNiV-gLuc-P2A-eGFP-infected supernatants were collected at 48 and 24 h.p.i., respectively, and titered by plaque assays on Vero-CCL81 cells. Fibroblasts infected with rVSV–GFP or rHSV-1-US11-GFP were fixed in 4% PFA 24 h.p.i. and stained with 5 μg ml−1 Hoechst 33342 in PBS. Cells were imaged and the results were analysed (BioTek Cytation 3; Gen5 software). HCMV-IE2-YFP-infected cells were fixed with 4% PFA 24 h.p.i., and imaging analysis was performed on an Acumen Explorer plate cytometer. Fibroblasts infected with IAV PR8-gLuc-PTV1 were collected and washed with PBS at 24 h.p.i. The cells were lysed and assayed for luciferase activity, according to the manufacturer’s instructions (Promega Renilla Luciferase Assay System; BioTek Cytation 3). We collected rSeV-gLuc-P2A-eGFP-infected supernatants were collected at 24 h.p.i. Supernatant samples were inactivated with lysis buffer, and assayed for luciferase activity according to the manufacturer’s instructions (Promega Renilla Luciferase Assay System; BioTek Cytation 3).
Co-housed 10-week-old female C57BL6/J (Jackson) and Isg15-deficient19 mice received intraperitoneal injections of PBS or 10,000 IU universal type-I IFN (PBL Interferon Source). At the post-treatment times indicated, the mice were sacrificed and exsanguinated for the analysis of ISG levels in whole blood. All procedures were carried out in accordance with National Institutes of Health and Icahn School of Medicine at Mount SinaiInstitutional Animal Care and Use Committee guidelines.
Plasmids and transfection
HEK-293 T cells were transiently transfected with Lipofectamine-2000 (Invitrogen), complexed with the following constructs according to the instructions provided by the manufacturer. Pseudotyped lentiviral particles were produced by transfection of pCAGGS-VSV-G, pCMV-Gag/Pol and pTRIP-hISG15ΔGG-IRES-RFP. The following constructs were used for expression and co-IP assays: pcDNA4b-huUSP18 (ref. 53), pcDNA3-3xFlag-huISG15 (from J. Huibregtse, University of Texas at Austin, TX, USA), pBabe-3xFlag-His6-huISG15ΔGG was derived from the construct above; pBK-CMV-mUsp18, pcDNA3-HA-mUsp18 and pCAGGS-Flag-mIsg15 (Addgene), pEGFP-N1 (Clontech), pcDNA4B-hUSP18-V5, and pcDNA4B-mUsp18-V5.
Murine LL171 cells were silenced by incubation for 24 h with 25 nM Isg15 siRNA (5′-CACAGUGAUGCUAGUGGUACA-3′; Sigma) followed by priming with IFN. HeLa and A549 cells were silenced by incubation for 24 h with 25 nM ISG15 siRNA followed by priming with IFN. siRNA transfections were performed with Lipofectamine RNAi max reagent (Invitrogen) according to the manufacturer’s instructions.
Cells were lysed in radioimmunoprecipitation assay buffer (RIPA buffer; 10 mM Tris-Cl, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS, 140 mM NaCl, 150 mM PMSF, protease inhibitor cocktail (Sigma-Aldrich), 1 μg ml−1 okadaic acid (Merck), and Halt phosphatase inhibitor (Thermo Scientific)) and analysed by western blot. For co-IP assays, transfected cells were lysed in 50 mM Tris pH 6.8, 0.5% Nonidet P40, 200 mM NaCl, 10% glycerol, 1 mM EDTA and a protease inhibitor cocktail. Sensitivity to NEM (Sigma-Aldrich) was assessed by adding this compound to the lysis buffer at a concentration of 10 mM. USP18 was immunoprecipitated with anti-human USP18 (Cell Signaling Technology D4E7, 1:250) or anti-V5 (Invitrogen R960-25, 1:200) monoclonal antibodies. The following antibodies were used for western blot analysis: human ISG15 (Santa Cruz 3E5, 1:1,000; ABGENT AP1150a, 1:1,000; mAb cl. 2.1, 1:1,000 (a gift from E.C. Borden, Cleveland Clinic, Cleveland OH54)); human USP18 (Cell Signaling Technology D4E7, 1:5,000); murine Isg15 (rabbit mAb 1551, 1:3,000; or rabbit pAbs, 1:5,000 (a gift from A.L. Haas, LSU Health Sciences Center School of Medicine, New Orleans, LA55); murine Usp18 (1:3,000 (a gift from K.P. Knobeloch, University of Freiburg, Freiburg, Germany56); V20 (Santa Cruz Biotechnology sc-50019, 1:200); murine Ifit1 (1:5,000) and Ifit2 (1:10,000; gifts from G. Sen (Cleveland Clinic, Cleveland, OH57); Stat1 (Millipore 06-501, 1:2,000); phospho-Stat1 (Cell Signaling Technology 9171 L, 1:1,000); murine Stat2 (1:5,000) (a gift from C. Schindler, Columbia University, NY, NY58); phospho-Stat2 (Millipore 07224, 1:1000); AKT (Cell Signaling Technology 40D4, 1:1000); actin (Sigma-Aldrich cl. AC-40, 1:10,000); HA (Sigma-Aldrich H9658, 1:2,000); Flag (Sigma-Aldrich F3165, 1:5,000); GFP (Rockland Immunochemicals 600-101-215, 1:1,000); V5 (Sigma-Aldrich V8137, 1:5,000). Antibody binding was detected by enhanced chemiluminescence (Western Lightning, Perkin Elmer). Relative band intensities were determined with a Fuji ImageQuant LAS-4000. Uncropped images for Fig. 4a and b can be found in Supplementary Fig. 5a–b. Uncropped images for Fig. 5a,e,f,h, , , can be found in Supplementary Fig. 6a–d (ref. 59).
How to cite this article: Speer, S. D. et al. ISG15 deficiency and increased viral resistance in humans but not mice. Nat. Commun. 7:11496 doi: 10.1038/ncomms11496 (2016).
This was supported in part by NIH grant R00 AI106942-02 to D.B., NIH grant R01 AI101820 to D.T., an American Heart Association pre-doctoral fellowship and a USPHS Institutional Research Training Award T32-AI07647 to T.J.G., NRSA T32 AR07279-30 to M.R.R., NIH grant RO1 A1080672 and Pew Scholar Award to D.J.L., funding by the DFG (SFB 944) to J.P., NIH grant R33 AI102267 to A.N.F. and B.L., CRIP (Center for Research on Influenza Pathogenesis), and NIAID funded Center of Excellence for Influenza Research and Surveillance (contract #HHSN272201400008C) to AGS. Experimental support was provided by the Speed Congenics Facility of the Rheumatic Disease Core Center (P30 AR048335). Work in the Cytokine Signaling Unit was supported by Institut Pasteur, CNRS, INSERM and an Amgen Scholarship to E.R.
FRAP of SiRSNAPf-mISG15 bound to mEGFP-mUSP18 Scale bar: 10 μm. Dynamic video demonstrating USP18 and ISG15 interactions in the micro patterning system as described for Supplementary Figure 3 and 4.
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