Figure 2: Preimplantation development of aneuploid embryos.

From: Mouse model of chromosome mosaicism reveals lineage-specific depletion of aneuploid cells and normal developmental potential

Figure 2: Preimplantation development of aneuploid embryos.
Figure 2

(a) Embryos were treated with reversine (n=20) and analysed for expression of lineage-associated genes at the early blastocyst stage, compared with controls (n=24). No difference in the number of Cdx2-positive and Nanog-positive cells was observed. (b) Embryos were treated with reversine (n=26) and analysed for lineage specification at the expanded blastocyst stage, compared with controls (n=31). Reversine-treated embryos have a significantly reduced cell number in all lineages (*P<0.05 and ***P<0.001; Student’s t-test), but the segregation of cell lineages was not affected. (c) Embryos were injected with Mad2 siRNA (n=22) or control siRNA (n=20) and analysed at the expanded blastocyst stage. Mad2 siRNA-injected embryos had significantly fewer cells than control siRNA-injected embryos (*P<0.05; Student’s t-test), but this effect was not as clear as that seen with reversine treatment. Scale bars, 20 μm. Error bars represent s.e.m.