Patch-clamp fluorometry measurements of cAMP binding and channel opening combined with global kinetic fitting revealed that ligand binding to the tetrameric hyperpolarization-activated cyclic nucleotide–gated channel is favored at the second and fourth binding events.
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Zhou, L. Cooperativity in twin gatings. Nat Chem Biol 8, 136–137 (2012). https://doi.org/10.1038/nchembio.776
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DOI: https://doi.org/10.1038/nchembio.776
