The assembly of chimeric synthetic pathways in cell factories will enable production of novel fuels and chemicals by microbial fermentation. The assembly of five enzymes for production of 1-butanol in Escherichia coli using kinetic considerations provides new lessons regarding these endeavors.
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Nielsen, J. Chimeric synthetic pathways. Nat Chem Biol 7, 195–196 (2011). https://doi.org/10.1038/nchembio.548
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DOI: https://doi.org/10.1038/nchembio.548
