Modification of folded proteins at will, within any sequence context, remains an elusive goal. A proteome-wide screening approach has now identified a set of protein ligases that enables conjugation of peptides to almost any protein N terminus, overcoming longstanding limitations in protein engineering.
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References
Weeks, A.M. & Wells, J.A. Nat. Chem. Biol. 14, 50–57 (2018).
Hermanson, G.T. Bioconjugation Techniques (Elsevier, 2013).
Popp, M.W. & Ploegh, H.L. Angew. Chem. Int. Ed. Engl. 50, 5024–5032 (2011).
Nguyen, G.K. et al. Nat. Chem. Biol. 10, 732–738 (2014).
Chang, T.K., Jackson, D.Y., Burnier, J.P. & Wells, J.A. Proc. Natl. Acad. Sci. USA 91, 12544–12548 (1994).
Nuijens, T. et al. Adv. Synth. Catal. 358, 4041–4048 (2016).
Schmidt, M., Toplak, A., Quaedflieg, P.J. & Nuijens, T. Curr. Opin. Chem. Biol. 38, 1–7 (2017).
Henager, H.H. et al. Nat. Methods 13, 925–927 (2016).
Schilling, O. & Overall, C.M. Nat. Biotechnol. 26, 685–694 (2008).
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Becker, C. Finding the best ligase. Nat Chem Biol 14, 2–3 (2018). https://doi.org/10.1038/nchembio.2533
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DOI: https://doi.org/10.1038/nchembio.2533
