Abstract
The bacterial pathogen Listeria monocytogenes uses the surface protein InlB to invade a variety of cell types1,2,3. The interaction of InlB with the hepatocyte growth-factor receptor, Met, is crucial for infection to occur3. Remarkably, the ubiquitin ligase Cbl is rapidly recruited to InlB-activated Met4. Recent studies have shown that ligand-dependent endocytosis of Met and other receptor tyrosine kinases is triggered by monoubiquitination of the receptor, a process that is mediatedc by Cbl5,6,7,8. Here, we show that purified InlB induces the Cbl-dependent monoubiquitination and endocytosis of Met. We then demonstrate that the bacterium exploits the ubiquitin-dependent endocytosis machinery to invade mammalian cells. First, we show that L. monocytogenes colocalizes with Met, EEA1, Cbl, clathrin and dynamin during entry. Then, we assess the role of different proteins of the endocytic machinery during L. monocytogenes infection. Over-expression or down-regulation of Cbl, respectively, increases or decreases bacterial invasion. Furthermore, RNA interference-mediated knock-down of major components of the endocytic machinery (for example, clathrin, dynamin, eps15, Grb2, CIN85, CD2AP, cortactin and Hrs), inhibit bacterial entry, establishing that the endocytic machinery is key to the bacterial internalization process.
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Acknowledgements
We thank A. de Melker and G. van der Horst from J. Borst's laboratory; and F. Brodsky, I. Dikic, T. Kirchhausen, M. A. McNiven and H. Stenmark for kindly providing us with reagents. We also thank M. Sachse, S. Sousa, E. Gouin and all members of P. Cossart's laboratory. This work received financial support from the Institut Pasteur (GPH 9), INSERM, ACI microbiologie and ARC. E.V. is a holder of an EMBO long-term fellowship. P.C. is an international research scholar from the Howards Hughes Medical Institute.
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Veiga, E., Cossart, P. Listeria hijacks the clathrin-dependent endocytic machinery to invade mammalian cells. Nat Cell Biol 7, 894–900 (2005). https://doi.org/10.1038/ncb1292
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DOI: https://doi.org/10.1038/ncb1292
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