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Small RNAs tell big stories in Whistler

Nature Cell Biology volume 10, pages 630–633 (2008)Cite this article

Abstract

The Keystone Symposium on RNAi, microRNA and non-coding RNA convened on March 25–30 at Whistler Resort in Whistler, British Columbia, Canada. Researchers with backgrounds in different biochemical disciplines came together to exchange ideas on short RNAs and their roles in a host of biological processes.

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Figure 1: The ping-pong model for piRNA biogenesis.
Figure 2: Model for miRNA biogenesis and function.

References

  1. Houwing, S. et al. A role for Piwi and piRNAs in germ cell maintenance and transposon silencing in Zebrafish. Cell 129, 69–82 (2007).

    Article  CAS  Google Scholar 

  2. Brennecke, J. et al. Discrete small RNA-generating loci as master regulators of transposon activity in Drosophila. Cell 128, 1089–1103 (2007).

    Article  CAS  Google Scholar 

  3. Yin, H. & Lin, H. An epigenetic activation role of Piwi and a Piwi-associated piRNA in Drosophila melanogaster. Nature 450, 304–308 (2007).

    Article  CAS  Google Scholar 

  4. Brower-Toland, B. et al. Drosophila PIWI associates with chromatin and interacts directly with HP1a. Genes Dev. 21, 2300–2311 (2007).

    Article  CAS  Google Scholar 

  5. Tam, O. H. et al. Pseudogene-derived small interfering RNAs regulate gene expression in mouse oocytes. Nature advance online publication, doi.10.1038/nature06904 (10 April 2008).

  6. Yigit, E. et al. Analysis of the C. elegans Argonaute family reveals that distinct Argonautes act sequentially during RNAi. Cell 127, 747–757 (2006).

    Article  CAS  Google Scholar 

  7. Lewis, B. P., Shih, I. H., Jones-Rhoades, M. W., Bartel, D. P. & Burge, C. B. Prediction of mammalian microRNA targets. Cell 115, 787–798 (2003).

    Article  CAS  Google Scholar 

  8. Lewis, B. P., Burge, C. B. & Bartel, D. P. Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell 120, 15–20 (2005).

    Article  CAS  Google Scholar 

  9. Krek, A. et al. Combinatorial microRNA target predictions. Nature Genet. 37, 495–500 (2005).

    Article  CAS  Google Scholar 

  10. Brennecke, J., Stark, A., Russell, R. B. & Cohen, S. M. Principles of microRNA-target recognition. PLoS Biol. 3, e85 (2005).

    Article  Google Scholar 

  11. Ma, E., MacRae, I. J., Kirsch, J. F. & Doudna, J. A. Auto-inhibition of human Dicer by its internal helicase domain. J. Mol. Biol. (doi: 10.1016/j.jmb.2008.05.005).

  12. Sinkkonen, L. et al. MicroRNAs control de novo DNA methylation through regulation of transcriptional repressors in mouse embryonic stem cells. Nature Struct. Mol. Biol. 15, 259–267 (2008).

    Article  CAS  Google Scholar 

  13. Johnson, S. M., Lin, S. Y. & Slack, F. J. The time of appearance of the C. elegans let-7 microRNA is transcriptionally controlled utilizing a temporal regulatory element in its promoter. Dev. Biol. 259, 364–379 (2003).

    Article  CAS  Google Scholar 

  14. Moss, E. G. & Tang, L. Conservation of the heterochronic regulator Lin-28, its developmental expression and microRNA complementary sites. Dev. Biol. 258, 432–442 (2003).

    Article  CAS  Google Scholar 

  15. Nelson, P. T., Hatzigeorgiou, A. G. & Mourelatos, Z. miRNP:mRNA association in polyribosomes in a human neuronal cell line. RNA 10, 387–394 (2004).

    Article  CAS  Google Scholar 

  16. Thomson, J. M. et al. Extensive post-transcriptional regulation of microRNAs and its implications for cancer. Genes Dev. 20, 2202–2207 (2006).

    Article  CAS  Google Scholar 

  17. Suh, M. R. et al. Human embryonic stem cells express a unique set of microRNAs. Dev. Biol. 270, 488–498 (2004).

    Article  CAS  Google Scholar 

  18. Yu, J. et al. Induced pluripotent stem cell lines derived from human somatic cells. Science 318, 1917–1920 (2007).

    Article  CAS  Google Scholar 

  19. Thermann, R. & Hentze, M. W. Drosophila miR2 induces pseudo-polysomes and inhibits translation initiation. Nature 447, 875–878 (2007).

    Article  CAS  Google Scholar 

  20. Wang, B., Yanez, A. & Novina, C. D. MicroRNA-repressed mRNAs contain 40S but not 60S components. Proc. Natl Acad. Sci. USA 105, 5343–5348 (2008).

    Article  CAS  Google Scholar 

  21. Eulalio, A., Huntzinger, E. & Izaurralde, E. GW182 interaction with Argonaute is essential for miRNA-mediated translational repression and mRNA decay. Nature Struct. Mol. Biol. 15, 346–353 (2008).

    Article  CAS  Google Scholar 

  22. Duursma, A. M., Kedde, M., Schrier, M., le Sage, C. & Agami, R. miR-148 targets human DNMT3b protein coding region. RNA 14, 872–877 (2008).

    Article  CAS  Google Scholar 

  23. Berkhout, B. & Jeang, K. T. RISCy business: MicroRNAs, pathogenesis, and viruses. J. Biol. Chem. 282, 26641–26645 (2007).

    Article  CAS  Google Scholar 

  24. Eis, P. S. et al. Accumulation of miR-155 and BIC RNA in human B cell lymphomas. Proc. Natl Acad. Sci. USA 102, 3627–3632 (2005).

    Article  CAS  Google Scholar 

  25. Garzon, R. et al. Distinctive microRNA signature of acute myeloid leukemia bearing cytoplasmic mutated nucleophosmin. Proc. Natl Acad. Sci. USA 105, 3945–3950 (2008).

    Article  CAS  Google Scholar 

  26. Iorio, M. V. et al. MicroRNA gene expression deregulation in human breast cancer. Cancer Res. 65, 7065–7070 (2005).

    Article  CAS  Google Scholar 

  27. Yu, F. et al. let-7 regulates self renewal and tumorigenicity of breast cancer cells. Cell 131, 1109–1123 (2007).

    Article  CAS  Google Scholar 

  28. Wolfrum, C. et al. Mechanisms and optimization of in vivo delivery of lipophilic siRNAs. Nature Biotechnol. 25, 1149–1157 (2007).

    Article  CAS  Google Scholar 

  29. Rozema, D. B. et al. Dynamic PolyConjugates for targeted in vivo delivery of siRNA to hepatocytes. Proc. Natl Acad. Sci. USA 104, 12982–12987 (2007).

    Article  CAS  Google Scholar 

  30. McBride, J. L. et al. Artificial miRNAs mitigate shRNA-mediated toxicity in the brain: implications for the therapeutic development of RNAi. Proc. Natl Acad. Sci. USA 105, 5868–5873 (2008).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Amy C. Seila is at the Koch Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. acseila@MIT.edu,

    Amy C. Seila

  2. Phillip A. Sharp is in the Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. sharppa@MIT.edu,

    Phillip A. Sharp

Authors
  1. Amy C. Seila
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  2. Phillip A. Sharp
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Seila, A., Sharp, P. Small RNAs tell big stories in Whistler. Nat Cell Biol 10, 630–633 (2008). https://doi.org/10.1038/ncb0608-630

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