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High Level Expression of Recombinant Genes in Aspergillus Oryzae

Bio/Technology volume 6, pages 1419–1422 (1988)Cite this article

Abstract

We have modified a method for transformation of Aspergillus nidulans1 to work for Aspergillus oryzae using selection for the amdS and argB genes from Aspergillus nidulans. To direct the expression of recombinant genes in A. oryzae we have used an alpha-amylase promoter cloned from a high yielding strain of A. oryzae. The amounts of heterologous protein obtained make this system attractive for even moderately priced industrial enzymes. As an example, the Rhizomucor miehei derived aspartic proteinase was secreted with yields in excess of three grams per liter. The proteinase was slightly overglycosylated, which did not, however, alter the specific activity of the enzyme.

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Authors and Affiliations

  1. Novo Research Institute, DK-2880, Bagsvaerd, Denmark

    Tove Christensen, Helle Woeldike, Esper Boel, Steen B. Mortensen, Kirsten Hjortshoej, Lars Thim & Mogens T. Hansen

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  1. Tove Christensen
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Christensen, T., Woeldike, H., Boel, E. et al. High Level Expression of Recombinant Genes in Aspergillus Oryzae. Nat Biotechnol 6, 1419–1422 (1988). https://doi.org/10.1038/nbt1288-1419

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