There are growing concerns about the introgression and persistence of transgenes into weedy wild relatives of crops. To investigate the risk of such an occurrence, we have transferred a fitness enhancing and insecticidal Bacillus thuringiensis (Bt cry1Ac) transgene to oilseed rape (canola) Brassica napus (AACC) and then by hybridization to the wild relative Brassica rapa (AA) Canola with Bt transgenes in various loci are introgressed into B. rapa from rates ranging from very high to zero, indicating that when transgenes are on the A genome, they can be easily moved into B. rapa, where they can persist. Transgenes located on the C genome are not able to be introgressed into B. rapa. Furthermore, transgenes are expressed at equitable levels in hybrids and Bt introgressed B. rapa as they are in the Bt canola parents. Therefore, we have constructed plasmids with linked Bt and green fluorescent protein (GFP) in plant transformation vectors to test the effectiveness of using GFP to monitor transgene flow and persistence in this system. Like Bt, GFP expression is also at equivalent levels in hybrids and introgressed B. rapa as compared with the canola parent. These tools will enable upcoming large field experiments to be performed to better assess the ecology and consequences of transgene escape into wild relatives.