Abstract
We describe the design and demonstrate the application of a modular integrated fluidized bed bioreactor system. Basically the system is a reactor vessel equipped with an extending cylinder and a liquid distributor plate. Instead of an external recirculation loop, as used in existing fluidized bed systems, a low shear stress impeller is used as the recirculation pump. The system has several unique features, such as modular exchangeable elements, efficient oxygenation and the option of operating as a stirred tank–, a packed bed– or a fluidized bed reactor. An example of a fluidized bed run using CHO–K1 cells is shown. Under standard culture conditions a 100–fold increase in cell density (up to 1.2 × 108 cells/ml) was achieved.
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Reiter, M., Blüml, G., Gaida, T. et al. Modular Integrated Fluidized Bed Bioreactor Technology. Nat Biotechnol 9, 1100–1102 (1991). https://doi.org/10.1038/nbt1191-1100
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DOI: https://doi.org/10.1038/nbt1191-1100
