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HLA Typing Using DNA Probes

Bio/Technology volume 4, pages 975–981 (1986)Cite this article

Abstract

We discuss the genetic organization of the human major histocompatibility complex (the HLA region), and review recent advances in HLA typing achieved by analysis at the DNA level. The use of HLA Class II DNA probes, labelled either with 32P or non–radioactively, has revealed polymorphisms not detectable with conventional serologic typing reagents. These DNA polymorphisms, identified by restriction fragment length analyses, or by allele–specific oligonucleotide probes, have proved informative in transplantation tissue–typing, paternity determinations, and for disease susceptibility studies.

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Authors and Affiliations

  1. Department of Human Genetics, Cetus Corporation, 1400 Fifty-Third Street, Emeryville, CA, 94608

    Henry A. Erlich, Edward L. Sheldon & Glenn Horn

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  1. Henry A. Erlich
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  2. Edward L. Sheldon
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  3. Glenn Horn
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Erlich, H., Sheldon, E. & Horn, G. HLA Typing Using DNA Probes. Nat Biotechnol 4, 975–981 (1986). https://doi.org/10.1038/nbt1186-975

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