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A Method for Fast and Pure DNA Elution from Agarose Gels by Centrifugal Filtration

Bio/Technology volume 3, pages 1014–1016 (1985)Cite this article

Abstract

A rapid and simple method for the recovery of DNA fragments from an agarose gel is described. It involves a ten minute centrifugation of the DNA-containing gel layered on a GeneScreen (NEN) or a Durapore (Millipore, GVWP 04700) membrane. With the latter a recovery of about 70 percent has been obtained with DNA fragments in the range of 0.4 to 25 Kbp. The eluted DNA stays intact and has been used successfully for ligation, restriction digestion, fill-in reaction by Klenow polymerase, tailing and for priming reverse transcription. Therefore, it is particularly useful for construction of plasmids and for other recombinant DNA research.

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Authors and Affiliations

  1. Laboratory of Molecular Biology, State University of Ghent, Ledeganckstraat 35, B-9000, Ghent, Belgium

    Jingdong Zhu, Walter Kempenaers, Dominique Van der Straeten, Roland Contreras & Walter Fiers

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  1. Jingdong Zhu
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  2. Walter Kempenaers
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  3. Dominique Van der Straeten
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  4. Roland Contreras
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  5. Walter Fiers
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Zhu, J., Kempenaers, W., Van der Straeten, D. et al. A Method for Fast and Pure DNA Elution from Agarose Gels by Centrifugal Filtration. Nat Biotechnol 3, 1014–1016 (1985). https://doi.org/10.1038/nbt1185-1014

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