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Identification of a calcium channel modulator using a high throughput yeast two-hybrid screen

Nature Biotechnology volume 16, pages 946–950 (1998)Cite this article

An Erratum to this article was published on 01 November 1998

Abstract

The interaction of the N-type calcium channel β3 subunit with the α1B subunit alters the activation/inactivation kinetics and the maximal conductance of the channel. The defined protein-protein interaction of the human α1B and β3 subunits provides a target for small-molecule modulation of N-type channel activity. We describe a high throughput screen based on a counterseiection yeast two-hybrid assay, which was used to identify small molecules that disrupt α1B-β3 subunit interactions and inhibit N-type calcium channel activity. These small molecules may be a new class of calcium channel antagonists with therapeutic potential.

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Author notes

  1. Rodrigo Franco: Corresponding author (e-mail: francor@war.wyeth.com).

Authors and Affiliations

  1. Wyeth-Ayerst Research, CNS Disorders, Princeton, NJ, 08543

    Stephen Lin, Lucy Sun, Eunice Lee, Mita Modi, Morris Husbands & Rodrigo Franco

  2. Cyanamid Agricultural Research Center, Molecular Genetic Screen Design, Princeton, NJ, 08543

    Kathleen Young, Samuel Hellings & Brad Ozenberger

  3. Wyeth-Ayerst Research, CNS/MCB, Princeton, NJ, 08543

    Kathleen Young & Brad Ozenberger

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  1. Kathleen Young
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Young, K., Lin, S., Sun, L. et al. Identification of a calcium channel modulator using a high throughput yeast two-hybrid screen . Nat Biotechnol 16, 946–950 (1998). https://doi.org/10.1038/nbt1098-946

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