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High Level Expression of Streptokinase in Escherichia Coli

Bio/Technology volume 10, pages 1138–1142 (1992)Cite this article

Abstract

Streptokinase (SK), which activates human plasminogen by promoting its conversion to plasmin, is normally obtained from β–hemolytic streptococci. Treatment with SK is an effective therapy for improving survival and preserving left ventricular function after coronary thrombosis. We report the cloning, expression in E. coli to levels of 25% of the total cell protein, and characterization of a novel SK (SKC–2) gene, the product of which is functionally equivalent to the naturally–derived protein. The availability of a recombinant streptokinase (rSK) in high yield and purity offers a potentially attractive alternative source of this important therapeutic agent.

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Author notes

  1. J. de la Fuente and L. Herrera: Corresponding author.

Authors and Affiliations

  1. Mammalian Cell Genetics Division, Centro de Ingeniería Genetica y Biotechnología, P.O. Box 6162, Havana, 6, Cuba

    M. P. Estrada, L. Hernandez, A. Pérez, P. Rodriguez, R. Serrano, R. Rubiera, A. Pedraza, J. de la Fuente & L. Herrera

  2. Analytical Unit, Centro de Ingeniería Genetica y Biotechnología, P.O. Box 6162, Havana, 6, Cuba

    G. Padrón & W. Antuch

Authors
  1. M. P. Estrada
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  2. L. Hernandez
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  3. A. Pérez
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  4. P. Rodriguez
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  5. R. Serrano
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  6. R. Rubiera
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  7. A. Pedraza
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  8. G. Padrón
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  10. J. de la Fuente
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  11. L. Herrera
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Estrada, M., Hernandez, L., Pérez, A. et al. High Level Expression of Streptokinase in Escherichia Coli. Nat Biotechnol 10, 1138–1142 (1992). https://doi.org/10.1038/nbt1092-1138

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