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Genetic selection and the lure of SIN

Nature Biotechnology volume 19, pages 824–825 (2001)Cite this article

A rapid method for exploring gene function uses alphavirus expression cloning.

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Figure 1: Library generation and selection using Sindbis vectors.

© Bob Crimi

References

  1. Koller, D. et al. Nat. Biotechnol. 19, 851–855 (2001).

    Article  CAS  Google Scholar 

  2. Seed, B. Curr. Opin. Biotechnol. 6,567–573 (1995).

    Article  CAS  Google Scholar 

  3. Rayner, J.R. & Gonda, T.J. Mol. Cell. Biol. 14, 880–887 (1994).

    Article  CAS  Google Scholar 

  4. Kitamura, T. et al. Proc. Natl. Acad. Sci. USA 92, 9146-9150 (1995).

  5. Kitamura, T. Int. J. Hematol. 67, 351–359 (1998).

    Article  CAS  Google Scholar 

  6. Roninson, I.B., Zuhn, D., Ruth, A. & de Graaf, D. Methods Enzymol. 292, 225–248 (1998).

    Article  CAS  Google Scholar 

  7. Xu, X. et al. Nat. Genet. 27, 23–29 (2001).

    Article  CAS  Google Scholar 

  8. Beger, C. et al. Proc. Natl. Acad. Sci. USA 98,130–135 (2001).

    Article  CAS  Google Scholar 

  9. Kavathas, P. et al. Proc. Natl. Acad. Sci. USA 81, 7688–7692 (1984).

    Article  CAS  Google Scholar 

  10. Kojima, T. & Kitamura, T. Nat. Biotechnol. 17, 487–490 (1999).

    Article  CAS  Google Scholar 

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  1. associate professor in the Department of Molecular Pharmacology and Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, 94305, CA

    Garry P. Nolan

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  1. Garry P. Nolan
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Nolan, G. Genetic selection and the lure of SIN. Nat Biotechnol 19, 824–825 (2001). https://doi.org/10.1038/nbt0901-824

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