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Computer Based Visualization for Quantitative and Qualitative Analysis of the Distribution of Matrix-Bound Proteins

Bio/Technology volume 6, pages 927–929 (1988)Cite this article

Abstract

We have developed a computer based imaging approach for determining the distribution of immobilized protein within a porous support. The computor-based visualization allows quantitative and qualitative analysis of the protein distribution profile. Microscopic fluorometry of fluorescein isothicyanate-labeled bovine serum albumin immobilized onto Eupergit C beads was used to examine the capability of the visualization method. As the total amount of immobilized protein increased with time. the gradient of the immobilized protein profile versus the radial position decreased, becoming virtually independent of the position at about 3 hours. This technique may be implemented with any protein detection methods using staining fluorescent or radioactive labels.

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Author information

Author notes

  1. Claudy J-P Mullon

    Present address: Research Division, W.R. Grace & Co., Lexington, MA, 01803

  2. W. Mark Saltzman

    Present address: Department of Chemical Engineering, Johns Hopkins University, Baltimore, MD, 21218

Authors and Affiliations

  1. Department of Chemical Engineering, M.I.T., Cambridge, MA, 02139

    Claudy J-P Mullon

  2. Division of Health Sciences and Technology, Harvard University/M.I.T., Cambridge, MA, 02139

    W. Mark Saltzman

  3. Department of Chemical Engineering, M.I.T., Cambridge, MA, 02139

    Robert Langer

  4. Harvard/M.I.T. Division of Health Sciences and Technology, Whitaker College of Health Sciences and Department of Surgery, Boston Children's Hospital,

    Robert Langer

Authors
  1. Claudy J-P Mullon
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  2. W. Mark Saltzman
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  3. Robert Langer
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Mullon, CP., Saltzman, W. & Langer, R. Computer Based Visualization for Quantitative and Qualitative Analysis of the Distribution of Matrix-Bound Proteins. Nat Biotechnol 6, 927–929 (1988). https://doi.org/10.1038/nbt0888-927

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  • DOI: https://doi.org/10.1038/nbt0888-927

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